2009 Fiscal Year Final Research Report
Focused proteomics of phosphorylated proteins using tautomycetin and its application
| Project/Area Number |
19208011
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
UBUKATA Makoto Hokkaido University, 大学院・農学研究院, 教授 (60168739)
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| Co-Investigator(Renkei-kenkyūsha) |
MITSUHASHI Shinya 北海道大学, 大学院・農学研究院, 学術研究員 (60526672)
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| Research Collaborator |
SHIGETOMI Kenngo 北海道大学, 大学院・農学研究院, 学術研究員 (20547202)
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| Project Period (FY) |
2007 – 2009
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| Keywords | PP1 / TNFα / NF-κB / IKK / RPS3a |
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| Research Abstract |
We have conducted focused proteomics of phosphorylated proteins by using tautomycetin. Protein phosphatase 1 (PP1) was found to act as a kind of regulatory enzyme for signal transduction in mammalian cells. For example, it positively regulates TNFα/NF-κB pathway as well as Raf. In addition, we isolated ATM30, a substrate of both PP1 and ATM/ATR kinase. ATM30 was found to be a ribosomal protein RPS3A(a) by MALDI-TOF/MS analysis.
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