Developments of transmission-blocking vaccine for tick-borne protozoan diseases

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19208026
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Kagoshima University
• Principal Investigator: FUJISAKI Kouzo Kagoshima University, 農学部, 教授 (00292095)
• Project Period (FY): 2007 – 2010
• Keywords: マダニ / Babesia原虫 / マダニ生物活性分子 / TBM / アミノ酸 / TOR / オートファジー / ワクチン

Research Abstract

本研究は、研究代表者らが分離・特性解明に成功している各種のマダニ生物活性分子(TBM)に関する最新知見を活用し、またB.gibsoniのESTデータベースも併用することによって、ガメートからスポロゾイトに至るマダニ体内の全発育期(SS期)虫体について、各種TBMと反応・結合する原虫分子の探索・同定、特性解明を展開し、最終的にこれらの組換え体複数を用いて、マダニ媒介性原虫病に特有の経発育期・介卵伝播を多段的・多重的に阻止しうる、世界初の多価疾病媒介阻止ワクチン(multi-component transmission-blocking vaccine : TBVs)の実現を図ることである。

Research Products

• [Journal Article] Hemalin, a thrombin inhibitor isolated from a midgut cDNA library from the hard tick Haemaphysalis longicornis (2009)
  • Author(s): Liao M, Zhou J, Gong H, Boldbaatar D, Shirafuji-Umemiya R, Battur B, Dong J, Nishikawa Y, Fujisaki K
  • Journal Title: J.Insect Physiol. 55 Pages: 165-174
  • Peer Reviewed
• [Journal Article] Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis (2009)
  • Author(s): Zhou J, Liao M, Ueda M, Gong H, Xuan X, Fujisaki K
  • Journal Title: Vet.Parasitol. 160 Pages: 180-183
  • Peer Reviewed
• [Journal Article] Blocking the secretion of saliva by silencing the HlYkt gene in the tick Haemaphysalis longicornis (2009)
  • Author(s): Gong H, Umemiya R, Zhou J, Liao M, Zhang H, Jia H, Nishikawa Y, Xuan X, Fujisaki K
  • Journal Title: Insect Biochem.Mol.Biol. 39 Pages: 372-381
  • Peer Reviewed
• [Journal Article] The hard tick Kunitz inhibitor, Haemangin, is a novel modulator or angiogenesis and wound healing and is crucial for slower blood-feeding arthropods (2009)
  • Author(s): Islam MK, Tsuji N, Miyoshi T, Alim MA, Huang X, Hatta T, Fujisaki K
  • Journal Title: PLoS Pathogen 5 Pages: e1000497
  • Peer Reviewed
• [Journal Article] LKR/SDH plays important roles throughout the tick life cycle including a long starvation period (2009)
  • Author(s): Battur B, Boldbaatar D, Umemiya-Shirafuji R, Liao M, Battsetseg B, Baymbaa B, Fujisaki K
  • Journal Title: PLoS One 4 Pages: e7136
  • Peer Reviewed
• [Journal Article] Hemoglobinase activity of a cysteine protease from the ixodid tick Haemaphysalis longicornis (2009)
  • Author(s): Yamaji K, Tsuji N, Miyoshi T, Islam MK, Hatta T, Alim MA, Anisuzzaman, Takenaka A, Fujisaki K.
  • Journal Title: Parasitol.Int. 58 Pages: 232-237
  • Peer Reviewed
• [Journal Article] Cloning and molecular characterization of tick kynureine aminotransferase (HlKAT) from Haemaphysalis longicornis (Acari : Ixodidae) (2009)
  • Author(s): Battsetseg B, Boldbaatar D, Battur B, Xuan X, Fujisaki K
  • Journal Title: Prasitol.Res. 105 Pages: 669-679
  • Peer Reviewed
• [Journal Article] Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari : Ixodiae) (2009)
  • Author(s): You M, Fujisaki K
  • Journal Title: J.Vet.Med.Sci. 71 Pages: 709-712
  • Peer Reviewed
• [Journal Article] Tick vitellogenin receptor reveals critical role in oocyte development and transovarial transmission of Babesia parasite (2008)
  • Author(s): Boldbaatar B, Battsetseg B, Matuso T, Hatta T, Umemiya-Shirafuji R, Xuan X, Fujisaki K
  • Journal Title: Biochem.Cell Biol. 86 Pages: 331-344
  • Peer Reviewed
• [Journal Article] Autophagy in ticks (2008)
  • Author(s): Umemiya-Shirafuji R, Matsuo T, Fujisaki K
  • Journal Title: Meth.Enzymol. 451 Pages: 621-638
  • Peer Reviewed
• [Presentation] Identification and characterization of Forkhead traqnscription factor from the hard tick, Haemaphysalis longicornis (2010)
  • Author(s): Boldbaatar D, 白藤(梅宮)梨可, 田仲哲也, 松岡輝重, 藤崎幸蔵
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本生命科学大学
  • Year and Date: 20100300
• [Presentation] RNA干渉法によるフタトゲチマダニTOR様遺伝子の発現抑制 (2010)
  • Author(s): 白藤(梅宮)梨可, Boldbaatar D, Liao M, 藤崎幸蔵
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本生命科学大学
  • Year and Date: 20100300
• [Presentation] マダニの吸血消化の分子基盤 : 最近の話題 (2010)
  • Author(s): 藤崎幸蔵
  • Organizer: 第24回日本動物原虫病学会学術集会
  • Place of Presentation: 日本生命科学大学
  • Year and Date: 2010-03-28
• [Presentation] マダニによるバベシア原虫媒介の分子基盤 (2009)
  • Author(s): 藤崎幸蔵
  • Organizer: 第21回日本薬学会微生物シンポジウム
  • Place of Presentation: 福山大学
  • Year and Date: 20090900
• [Presentation] マダニカテプシンL様システインプロテアーゼの性状解析 (2009)
  • Author(s): 山地佳代子, 辻尚利, 三好猛晴, 八田岳士, Abdul M.Alim, M.Anisuzzanman, 櫛引史郎, 藤崎幸蔵
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取大学
  • Year and Date: 20090900
• [Presentation] 創薬開発のマダニ生物活性分子 (2009)
  • Author(s): 藤崎幸蔵
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 麻布大学
  • Year and Date: 20090400
• [Presentation] マダニのオートファジー研究の展開 : マダニとその媒介病原体の制圧に向けて (2009)
  • Author(s): 白藤(梅宮)梨可, 松尾智英, 藤崎幸蔵
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 麻布大学
  • Year and Date: 2009-04-02
• [Book] 図説獣医衛生動物学 (2009)
  • Author(s): 今井壮一, 藤崎幸蔵, 板垣匡, 森田達志
  • Total Pages: 44-100, 132-159, 183-196
  • Publisher: 講談社
• [Remarks] ホームページ
  • URL: http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html
• [Patent(Industrial Property Rights)] ダニのキチナーゼ (2010)
  • Inventor(s): 藤崎幸蔵, ほか7名
  • Industrial Property Rights Holder: 明治製菓、藤崎幸蔵
  • Industrial Property Number: 特許4452510号
  • Acquisition Date: 2010-02-05