2010 Fiscal Year Final Research Report
Developments of transmission-blocking vaccine for tick-borne protozoan diseases
| Project/Area Number |
19208026
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied veterinary science
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUJISAKI Kouzo Kagoshima University, 農学部, 教授 (00292095)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Tetsuya 鹿児島大学, 農学部, 准教授 (00322842)
SUZUKI Hiroshi 帯広畜産大学, 原虫病研究センター, 教授 (60333473)
TSUJI Naotoshi 独立行政法人農業食品産業技術総合研究機構, 動物衛生研究所, 主任研究員 (70355171)
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| Project Period (FY) |
2007 – 2010
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| Keywords | マダニ / Babesia原虫 / マダニ生物活性分子 / TBM / アミノ酸 / TOR / オートファジー / ワクチン |
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| Research Abstract |
(1) We could identify for the first time vitellogenins (Vg), vitellogeni-receptor (VgR), and longipain as tick-bioactive molecules (TBM) specifically reacted with sexual and sporogonic stages (S-S satages) of Babesia parasites developing in the vector ticks. (2) Since Vg and VgR could inhibit completely the Babesia transmission in ticks treated with their dsRNA and could suppress effectively the Babesia transmission in animals vaccinated with them, the identification of Babesia molecules reacted with them is now concentrated. (3) However, longipain could promote Babesia development in vector ticks after RNAi, it is considered that its application as candidate molecule for the transmission-blocking vaccine may be difficult under the current research situation lacking of the TBM over0expression systems. (4) During the period of this research, we started to focus on TBMs involved in cellular biomass (CB)control in host cells for Babesia parasites because it was noticed that the development of intra-cellular parasites is fundamentally influenced by the essential nutrients supply under the control of CB. (5) Therefore, the molecular identification and characterization of a series of TBMs involving in transcription, translation, post translational modification, and vesicular transport, autophagy (AT) of intra-cellular proteins are concentrated for the first time. (6) These studies are elucidating the significance of TOR pathways (TORs) as candidate molecules for the transmission-blocking vaccine.
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[Journal Article] Longistatin, a plasminogen activator, is key to the availability of blood meals for ixodid ticks.2011
Author(s)
Anisuzzman, Islam MK, Abdul MA, Miyoshi T, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N
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Journal Title
PLoS Pathogen 第7巻
Pages: e1001312
Peer Reviewed
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[Journal Article] Autophagy and its physiological relevance in arthropods : Current knowledge and perspectives.2010
Author(s)
Malagoli D, Abdalla FC, Cao Y, Feng Q, Fujisaki K, Gregorc A, Matsuo T, Nezis IP, Papassideri IS, Sass M, Silva-Zacarin ECM, Tettamanti G, Umemiya-Shirafuji R
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Journal Title
Autophagy 第6巻
Pages: 575-588
Peer Reviewed
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[Journal Article] Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes.2010
Author(s)
Hatta T, Tsuji N, Miyoshi T, Islam MK, Alim MA, Yamaji K, Anisuzzaman, Fujisaki K
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Journal Title
Parasitol Int. 第59巻
Pages: 286-289
Peer Reviewed
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[Journal Article] Longistatin, a novel EF-hand protein from the ixodid tick Haemaphysalis longicornis, is required for acquisition of host blood-meals.2010
Author(s)
Anisuzzaman, Islam MK, Miyoshi T, Alim MA, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N
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Journal Title
Int J Parasitol. 第40巻
Pages: 721-729
Peer Reviewed
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[Journal Article] Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks Insect Biochem.2008
Author(s)
Liao M, Boldbaatar D, Gong H, Huang P, Umemiya R, Harnnoi T, Zhou J, Tanaka T, Suzuki H, Xuan X, Fujisaki K
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Journal Title
Mol.Biol. 第38巻
Pages: 285-295
Peer Reviewed
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