From the ER to the plasma membrane : Vesicular transport of membrane skeleton units and the diseases

2009 Fiscal Year Final Research Report

• Project/Area Number: 19208027
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: INABA Mutsumi Hokkaido University, 大学院・獣医学研究科, 教授 (00183179)
• Co-Investigator(Kenkyū-buntansha): SATO Kota 北海道大学, 大学院・獣医学研究科, 准教授 (50283974) ; AGUI Takashi 北海道大学, 大学院・獣医学研究科, 教授 (00212457) ; INANAMI Osamu 北海道大学, 大学院・獣医学研究科, 教授 (10193559) ; OTSUKA Yayoi 北海道大学, 大学院・獣医学研究科, 博士研究員 (30396303) ; WATANABE Nobuhisa 北海道大学, 大学院・先端生命科学研究院, 准教授 (70212321) ; YAMAMOTO Masayuki 東北大学, 大学院・医学系研究科, 教授 (50166823) ; TAKAKUWA Yuichi 東京女子医科大学, 医学部, 教授 (40113740) ; OHTA Hiroshi 北海道大学, 大学院・獣医学研究科, 助教 (50431333) ; UMEMURA Takashi 北海道大学, 大学院・獣医学研究科, 教授 (00151936) ; HIKASA Yoshiaki 鳥取大学, 農学部, 教授 (30165071)
• Project Period (FY): 2007 – 2009
• Keywords: 細胞膜 / 小胞体 / トラフィック / 膜骨格 / 疾患 / 赤血球膜 / 遺伝

Research Abstract

The long-range goal of this project is to investigate the mechanism for assembly and functional organization of the red cell membrane skeleton. Here, we analyzed the synthesis, quality control, interaction with an erythroid scaffolding protein ankyrin in the endoplasmic reticulum (ER), and the ER exit signal of anion exchanger 1 (AE1) and its mutants. The results obtained together indicate that the membrane skeletal network is assembled in the ER membrane as a small unit followed by vesicular transport to the plasma membrane.

Research Products

• [Journal Article] The forced aggresome formation of a bovine anion exchanger 1 (AE1) mutant through assciation with DF508-cystic fibrosis transmembrane conductance-regulator upon proteasome inhibition in HEK293 cells (2010)
  • Author(s): Adachi, H., Takao Kurooka, Wataru Otsu, Inaba, M.
  • Journal Title: Jpn. J. Vet. Res. 58(in press)
  • Peer Reviewed
• [Journal Article] Extrusion of the Na, K-ATPase and the transferrin receptor in different population of exosomes with lipid raft-associated proteins during reticulcoyte maturation in dogs (2010)
  • Author(s): Komatsu, T., Arashiki, N., Otsuka, Y., Sato, K., Inaba, M.
  • Journal Title: Jpn. J. Vet. Res. 58 Pages: 17-27
  • Peer Reviewed
• [Journal Article] A novel copper (II) coordination at His186 in full-length murine prion protein (2010)
  • Author(s): Watanabe, Y., Hiraoka, W., Igarashi, M., Ito, K., Shimoyama, Y., Horiuchi, M., Yamamori, T., Yasui, H., Kuwabara, M., Inagaki, F., Inanami, O.
  • Journal Title: Biochem. Biophys. Res. Commun. 394 Pages: 522-528
  • Peer Reviewed
• [Journal Article] Parallel reductions in stomatin and Na, K-ATPase through the exosomal pathway during reticulocyte maturation in dogs : stomatin as a genotypic and phenotypic marker of high K^+ and low K^+ red cells (2010)
  • Author(s): Komatsu, T., Sato, K., Otsuka, Y., Arashiki, N., Tanaka, K., Tamahara, S., Ono, K., Inaba, M.
  • Journal Title: J. Vet. Med. Sci. 72(in press)
  • Peer Reviewed
• [Journal Article] Covalent modification of spectrin in red cell membranes by the lipid peroxidation product 4-hydroxy-2-nonenal (2010)
  • Author(s): Arashiki, N., Otsuka, Y., Ito, D., Yang, M., Komatsu, T., Sato, K., Inaba, M.
  • Journal Title: Biochem. Biophys. Res. Commun. 391 Pages: 1543-1547
  • Peer Reviewed
• [Journal Article] Amelioration of anemia in the ICGN mouse, a renal anemia model, with a subcutaneous bolus injection of erythropoietin adsorbed to hydroxyapatite matrix (2009)
  • Author(s): Nagasaki, K., Ikoma, T., Katsuda, S., Tonegawa, T., Tanaka, J., Ohyama, M., Hayashida, K., Nakamura, T., Sato, H., Ito, S., Sasaki, N., Agui T.
  • Journal Title: J. Vet. Med. Sci. 71 Pages: 1365-71
  • Peer Reviewed
• [Journal Article] Structural implications of the EL (K/Q) (L/C) LD (A/G) DD sequence in the C-terminal cytoplasmic tail for proper targeting of anion exchanger 1 to the plasma membrane (2009)
  • Author(s): Adachi, H., Ito, D., Kurooka, T., Otsuka, Y., Arashiki, N., Sato, K., Inaba, M.
  • Journal Title: Jpn. J. Vet. Res. 57 Pages: 135-146
  • Peer Reviewed
• [Journal Article] Marked difference in membrane-protein-binding properties of the two isoforms of protein 4.1R expressed at early and late stages of erythroid differentiation (2009)
  • Author(s): Nunomura, W., Parra, M., Hebiguchi, M., Sawada, K., Mohandas, N., Takakuwa, Y.
  • Journal Title: Biochem. J. 417 Pages: 141-148
  • Peer Reviewed
• [Journal Article] Stem cells of GATA1-related leukemia undergo pernicious changes after 5-fluorouracil treatment (2009)
  • Author(s): Abe, K., Shimizu, R., Pan, X., Hamada, H., Yoshikawa, H., Yamamoto, M.
  • Journal Title: Exp. Hematol. 37 Pages: 435-445
  • Peer Reviewed
• [Journal Article] Expression of α-hemoglobin stabilizing protein and cellular prion protein in a subclone of murine erythroleukemia cell line MEL. (2008)
  • Author(s): Otsuka, Y., Ito, D., Kastuoka, K., Arashiki, N., Komatsu, T., Inaba, M.
  • Journal Title: Jpn. J. Vet. Res. 56 Pages: 75-84
  • Peer Reviewed
• [Journal Article] Identification of genes for two major sialoglycoproteins, glycophorin A and glycophorin C in canine red cell membranes (2008)
  • Author(s): Sato, K., Otsuka, Y., Arashiki, N., Komatsu, T., Wang, C. -C., Tamahara, S., Inaba, M.
  • Journal Title: Jpn. J. Vet. Res. 55 Pages: 103-114
  • Peer Reviewed
• [Presentation] 蛍光相関分光法を用いた膜骨格蛋白質 protein 4.1の細胞内動態の観察 (2010)
  • Author(s): 田中正太郎, 高桑雄一
  • Organizer: 日本膜学会第32年会
  • Place of Presentation: 産業技術総合研究所臨界副都心センター
  • Year and Date: 2010-05-14
• [Presentation] Characterization of glycophorins A and B involved in the major high molecular weight sialoglycoprotein in bovine red cell membranes (2009)
  • Author(s): Otsuka, Y., Otsu, W., Arashiki, N., Kurogi, K., Sato, K., Inaba, M.
  • Organizer: 日本生化学会第82回大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] The role of the N-terminal region of the cytoplasmic domain containing the ΦXΦXΦ sequence in the trafficking of erythroid anion exchanger I to the plasma membrane (2009)
  • Author(s): Otsu, W., Otsuka, Y., Kurooka, T., Sato, K., Inaba, M.
  • Organizer: 日本生化学会第82回大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-23
• [Presentation] 牛赤血球膜グライコフォリンの同定 : 高分子多型と血液型抗原の形成 (2009)
  • Author(s): 大塚弥生, 新敷信人, 黒木一仁, 佐藤耕太, 稲葉睦
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取とりぎん会館
  • Year and Date: 2009-09-25
• [Presentation] The role of the N-terminal region of the cytoplasmic domain containing the ΦXΦXΦ sequence in the trafficking of erythroid anion exchanger 1 to the plasma membrane (2009)
  • Author(s): Inaba, M., Otsu, W., Otsuka, Y., Sato, K.
  • Organizer: Gordon Research Conferences, Red Cell
  • Place of Presentation: New England University, MA, USA.
  • Year and Date: 2009-06-25
• [Presentation] 牛赤血球グライコフォリンA&B : 性状と血液型抗原との関連 (2009)
  • Author(s): 大塚弥生, 新敷信人, 小松智彦, 大津航, 黒木一仁, 佐藤耕太, 稲葉睦
  • Organizer: 日本膜学会第30年会
  • Place of Presentation: 東京理科大学森戸記念館
  • Year and Date: 2009-05-22
• [Presentation] 赤血球型AE1の膜トラフィック : N末端ΦXΦXΦ配列による ER-Golgi 間輸送制御 (2009)
  • Author(s): 大津航, 安達啓一, 大塚弥生, 佐藤耕太, 稲葉睦
  • Organizer: 日本膜学会第30年会
  • Place of Presentation: 東京理科大学森戸記念館
  • Year and Date: 2009-05-22
• [Presentation] AE1の細胞内輸送における細胞質内領域モチーフの役割 (2008)
  • Author(s): 大津航, 安達啓一, 大塚弥生, 佐藤耕太, 稲葉睦
  • Organizer: 日本生化学会第80回大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-11
• [Presentation] 手法で語る生体膜研究 (2008)
  • Author(s): 稲葉睦
  • Organizer: 日本膜学会第29年会
  • Place of Presentation: 東京理科大学森戸記念館
  • Year and Date: 2008-05-16
• [Presentation] AE1の細胞内輸送を規定する細胞質ドメインの疎水性クラスター (2008)
  • Author(s): 大津航, 安達啓一, 大塚弥生, 佐藤耕太, 稲葉睦
  • Organizer: 日本膜学会第29年会
  • Place of Presentation: 東京理科大学森戸記念館
  • Year and Date: 2008-05-16
• [Presentation] 牛の遺伝性球状赤血球症 : バンド3 (AE1) 欠損に基づく病態と応用研究 (シンポジウム「血液学研究における疾患動物モデルレの作製と応用」) (2007)
  • Author(s): 稲葉睦
  • Organizer: 第69回日本血液学会・第49回日本臨床血液学会合同総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-11
• [Book] Schalm's Veterinary Hematology, 6th ed. (Chapter 29 : Erythrocyte membrane defects., Weiss, D. and Wardrop, K. J. eds.) (2010)
  • Author(s): Inaba, M., Messick, J. B.
  • Total Pages: 187-195
  • Publisher: Wiley-Blackwell
• [Remarks] ホームページ等 主催関連研究会 : 獣医臨床遺伝研究会
  • URL: http://square.umin.ac.jp/jsacg/