2009 Fiscal Year Final Research Report
Chemical Biology in internalization of membrane-permeable peptides
| Project/Area Number |
19209004
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Drug development chemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
FUTAKI Shiroh Kyoto University, 化学研究所, 教授 (50199402)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKASE Ikuhiko 京都大学, 化学研究所, 助教 (40432322)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | アルギニンペプチド / 膜透過ペプチド / マクロピノサイトーシス / 受容体 / 細胞内移行 / 細胞膜 / 光反応性架橋団 / 薬物送達 |
|---|
| Research Abstract |
Involvement of macropinocytosis to the internalization of arginine-rich membrane-permeable peptides has been suggested. In this study, using an R12 peptide bearing diazirine-based photo-reactive crosslinkers, a potential receptor to induce macropinocytosis by the interaction with R12 was identified. It was also elucidated that internalization efficiency of arginine-rich peptides are greatly affected by the peptide concentration, presence of serum, addition of hydrophobic segments and so on.
|
