2010 Fiscal Year Final Research Report
Development of a novel detection method for abnormal prion protein
| Project/Area Number |
19209020
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
KAI Masaaki Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (00160953)
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| Co-Investigator(Renkei-kenkyūsha) |
KABASHIMA Tsutomu 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20274673)
SHIBATA takayuki 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10448491)
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| Project Period (FY) |
2007 – 2010
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| Keywords | プリオン / アプタマー / 感染症 |
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| Research Abstract |
The aim of this research is to develop the novel detection method for the abnormal prion protein, which causes the prion disease. The recombinant normal prion protein was expressed in E. coli, and purified with affinity chromatography. The denatured prion protein, which indicates resistance against protease digestion, was prepared by the treatment of the normal prion protein with cupric ions. In this project, we developed highly sensitive and specific detection methods for the prion proteins on a solid phase membrane using nucleic aptamer and chemiluminescent reagents.
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