Identification of genes responsible for Down syndrome using mice harboring a human artfuciak chromosome (HAC).

2009 Fiscal Year Final Research Report

• Project/Area Number: 19209038
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Keio University
• Principal Investigator: KUDOH Jun Keio University, 医学部, 教授 (80178003)
• Co-Investigator(Kenkyū-buntansha): IKENO Masashi 慶應義塾大学, 医学部, 講師 (80298546) ; SHIBUYA Kazunori 慶應義塾大学, 医学部, 助教 (90296723) ; SAKAI Kosuke 慶應義塾大学, 医学部, 助教 (70245463)
• Research Collaborator: SHIMIZU Nobuyoshi 慶應義塾大学, 名誉教授 ; ASAKAWA Shuichi 東京大学, 農学部, 教授 ; OKAZAKI Tuneko 名古屋大学, 名誉教授 ; SUZUKI Nobutaka 慶應義塾大学, 医学部, 助教 ; MIYAMOTO Kenichi 慶應義塾大学, 医学部, 助教
• Project Period (FY): 2007 – 2009
• Keywords: ダウン症 / トリソミー21 / ヒト21番染色体 / 疾患モデルマウス / HAC / BAC / マウス16番染色体 / 顕微注入法

Research Abstract

In this study, we attempted to produce novel Down syndrome model mice. We therefore took advantage of gene map of human chromosome 21 which we have brushed up over a decade, bacterial artificial chromosome (BAC) clones, and human artificial chromosome (HAC) which has been developed recently.

Research Products

• [Presentation] Production of mice harboring a human artificial chromosome (HAC) and expression of human genes in mouse tissues. (2009)
  • Author(s): Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M., Kudoh J.
  • Organizer: The 32nd Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 20091209-20091212
• [Presentation] Novel method to produce partial trisomy mice using a human artificial chromosome (HAC); an application to Down syndrome. (2009)
  • Author(s): Kudoh J., Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M.
  • Organizer: The 59th Annual Meeting of ASHG
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 20091020-20091024
• [Presentation] Production of Down syndrome mouse model harboring a human artificial chromosome (HAC) including a bacterial artificial chromosome (BAC). (2009)
  • Author(s): Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M., Kudoh J.
  • Organizer: The 54th Annual Meeting of the Japan Society of Human Genetics
  • Place of Presentation: Shinagawa
  • Year and Date: 20090923-20090926
• [Presentation] Expression of human genes on a human artificial chromosome (HAC) transduced into mice. (2008)
  • Author(s): Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M., Kudoh J.
  • Organizer: BMB2008 Biochemistry and Molecular Biology
  • Place of Presentation: Kobe
  • Year and Date: 20081209-20081212
• [Presentation] Identification of genes responsible for Down syndrome using mice harboring a human artificial chromosome (HAC). (2008)
  • Author(s): Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M., Kudoh. F
  • Organizer: G-COE Workshop
  • Place of Presentation: Shinanomachi
  • Year and Date: 2008-02-29
• [Presentation] Identification of genes responsible for Down syndrome using mice harboring a human artificial chromosome (HAC). (2007)
  • Author(s): Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M., Kudoh
  • Organizer: BMB2007 Biochemistry and Molecular Biology
  • Place of Presentation: Yokohama
  • Year and Date: 20071211-20071215
• [Presentation] Production of Novel Down Syndrome Mouse Model using Human Artificial Chromosome (HAC). (2007)
  • Author(s): Kudoh J., Miyamoto K., Suzuki N., Sakai K., Asakawa S., Okazaki T., Shimizu N., Ikeno M.
  • Organizer: Expert Workshop on the Biology of Chromosome 21 Genes
  • Place of Presentation: Washington D.C.
  • Year and Date: 20070928-20071001