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2009 Fiscal Year Final Research Report

Analysis of the locomotor circuit formation in zebrafish spinal cord

Research Project

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Project/Area Number 19300118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionNational Institutes of Natural Sciences Okazaki Research Facilities

Principal Investigator

HIGASHIJIMA Shin-ichi  National Institutes of Natural Sciences Okazaki Research Facilities, 岡崎統合バイオサイエンスセンター, 准教授 (80270479)

Project Period (FY) 2007 – 2009
Keywordsゼブラフィッシュ / 神経分化 / 神経回路 / トランスジェニック / 脊髄
Research Abstract

The p2 progenitor domain in the ventral spinal cord gives rise to two interneuron subtypes, V2a and V2b. The mechanism as to how V2a and V2b neurons arise from a single progenitor domain was unknown. We showed in this study that p2 progenitors divide once to produce V2a/V2b neuron pairs, indicating that V2a and V2b neurons are generated by the asymmetric division of pair-producing progenitor cells. We also showed that Delta-Notch interactions between sister cells play a critical role in the final outcome of these asymmetric divisions. This mechanism for determining cell fate is similar to asymmetric divisions that occur during Drosophila neurogenesis, where ganglion mother cells divide once to produce distinct neurons. However, unlike in Drosophila, the divisional axes of p2 progenitors in zebrafish were not fixed. We report that the terminal division of pair-producing progenitor cells in vertebrate neurogenesis can reproducibly produce two distinct neurons through a mechanism that may not depend on the orientation of the division axis.

  • Research Products

    (24 results)

All 2010 2009 2008 2007

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (9 results) Book (4 results)

  • [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum.2010

    • Author(s)
      Kani, S., Bae, Y-K, Shimizu T, Tanabe, K., Satou, C., Parsons, M., Scott, E., Baier, H., Higashijima, S., Hibi, M.
    • Journal Title

      Developmental Biology (in press)

    • Peer Reviewed
  • [Journal Article] Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish.2010

    • Author(s)
      Wada, H., Ghysen, A., Satou, C., Higashijima, S, Kawakami, K., Hamaguchi, S., Sakaizumi, M.
    • Journal Title

      Developmental Biology 340

      Pages: 583-594

    • Peer Reviewed
  • [Journal Article] Illuminating Cell-Cycle Progression in the Developing Zebrafish Embryo.2009

    • Author(s)
      Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A.
    • Journal Title

      Proc. Natl. Acad. Sci. (USA) 106

      Pages: 20812-20817

    • Peer Reviewed
  • [Journal Article] Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish.2009

    • Author(s)
      Satou, C., Kimura, Y., Kohashi, T., Horikawa, K., Takeda, H., Oda, Y., Higashijima, S.
    • Journal Title

      J. Neuroscience 29

      Pages: 6780-6793

    • Peer Reviewed
  • [Journal Article] Anatomy of zebrafish cerebellum and screen for mutations affecting its development.2009

    • Author(s)
      Bae, Y., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S., Hibi, M.
    • Journal Title

      Developmental Biology 300

      Pages: 406-426

    • Peer Reviewed
  • [Journal Article] From the Olfactory Bulb to Higher Brain Centers: Genetic Visualization of Secondary Olfactory Pathways in Zebrafish.2009

    • Author(s)
      Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., Yoshihara, Y.
    • Journal Title

      J. Neuroscience 29

      Pages: 4756-4767

    • Peer Reviewed
  • [Journal Article] Vsx2 in the zebrafish retina: restricted lineages through depression.2009

    • Author(s)
      Vitorino, M., Jusf, P.R., Maurus, D., Kimura, Y., Higashijima, S., Harris, W.A.
    • Journal Title

      Neural Development 4

      Pages: 14

    • Peer Reviewed
  • [Journal Article] Mutation in the abdb gene causes abnormal iron and fatty acid metabolism in developing medaka fish.2008

    • Author(s)
      Miyake, A., Higashijima, S., Kobayashi, D., Narita, T, Jindo, T., Setiamarga, D.H.E., Ohisa, S., Orihara, N, Hibiya, K., Konnno, S., Sakaguchi, S., Hoie, K., Imai, Y., Naruse, K., Kudo, A., Takeda, H.
    • Journal Title

      Development, Growth & Differentiation 50

      Pages: 703-716

    • Peer Reviewed
  • [Journal Article] V2a and V2b neurons are generated by the final divisions of pair-producing progenitors in the zebrafish spinal cord.2008

    • Author(s)
      Kimura, Y., Satou, C., Higashijima, S.
    • Journal Title

      Development 135

      Pages: 3001-3005

    • Peer Reviewed
  • [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs.2008

    • Author(s)
      Hossain, Md. I., Iwasaki, H., Okouchi, Y., Chahine, M., Higashijima, S., Nagayama, K., Okamura, Y.
    • Journal Title

      J. Biol. Chem 283

      Pages: 18248-59

    • Peer Reviewed
  • [Journal Article] A topographic map of recruitment in spinal cord.2007

    • Author(s)
      McLean, D.L., Fan, J., Higashijima, S., Hale, M.E., Fetcho, J.R.
    • Journal Title

      Nature 446

      Pages: 71-5

    • Peer Reviewed
  • [Presentation] ゼブラフィッシュを用いたin vivoイメージング2009

    • Author(s)
      東島眞一
    • Organizer
      第32回日本分子生物学会年会ワークショップ
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      S. Higashijima
    • Organizer
      3rd Viktor Hamburger Symposium
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2009-10-16
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      S Higashijima, C Satou, Y Kimura
    • Organizer
      The 32th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2009-09-16
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2009

    • Author(s)
      S Higashijima, Y Kimura, C Satou
    • Organizer
      IUPS2009
    • Place of Presentation
      Kyoto Japan
    • Year and Date
      2009-07-31
  • [Presentation] V2a and V2b neurons are generated by the final divisions of pair-producing progenitors.2008

    • Author(s)
      Kimura Y, Satou C, Higashijima S
    • Organizer
      Neuroscience 2008
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2008-11-18
  • [Presentation] Analysis of spinal V0 neurons with transgenic fish.2008

    • Author(s)
      Satou C, Kimura Y, Higashijima S
    • Organizer
      Neuroscience 2008
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2008-11-17
  • [Presentation] ゼブラフィッシュのロコモーションの成魚に関わる脊髄神経回路の分子的基盤2008

    • Author(s)
      東島眞一, 木村有希子, 佐藤千恵
    • Organizer
      第80回日本遺伝学会ワークショップ
    • Place of Presentation
      名古屋
    • Year and Date
      2008-09-04
  • [Presentation] Properties and functions of spinal V0 neurons in zebrafish.2008

    • Author(s)
      Satou C, Kimura Y, Higashijima S
    • Organizer
      Neuro2008
    • Place of Presentation
      Tokyo
    • Year and Date
      2008-07-09
  • [Presentation] Development and function of spinal locomotor circuits in zebrafish2007

    • Author(s)
      東島眞一, 木村有希子, 佐藤千恵
    • Organizer
      第30回日本神経科学大会シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2007-09-11
  • [Book] 細胞工学2009

    • Author(s)
      東島眞一
    • Total Pages
      107
    • Publisher
      秀潤社
  • [Book] Development, Growth & Differentiation(Transgenic zebrafish expressing fluorescent proteins in central nervous system neurons.)2008

    • Author(s)
      Higashijima, S.
    • Total Pages
      407-413
  • [Book] Brain Research Reviews(Zebrafish and motor control over the last decade.)2008

    • Author(s)
      Fetcho, J. R., Higashijima, S., McLean, D. L.
    • Total Pages
      86-93
  • [Book] 実験医学増刊号 蛍光・発光試薬の選び方と使い方(in vivoイメージングのためのプローブ~ゼブラフィッシュの蛍光イメージング)2007

    • Author(s)
      木村有希子、佐藤千恵、東島眞一
    • Total Pages
      125-130
    • Publisher
      羊土社

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Published: 2011-06-18   Modified: 2016-04-21  

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