2009 Fiscal Year Final Research Report
Real-time imaging of stress-related receptor dynamics in the brain
| Project/Area Number |
19300120
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NISHI Mayumi Kyoto Prefectural University of Medicine, 医学部, 教授 (40295639)
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| Co-Investigator(Kenkyū-buntansha) |
金城 政孝 北海道大学, 大学院・先端生命科学研究院, 教授 (70177971)
藤川 和世 京都府立医科大学, 医学研究科, プロジェクト研究員 (40433247)
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| Project Period (FY) |
2007 – 2009
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| Keywords | GFP / GR / MR / ノックインマウス / in vivoイメージング / 海馬 / ストレス / 核輸送 |
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| Research Abstract |
The ultimate aim of the present study is to clarify how glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) in the brain respond to hormonal milieu fluctuated during stress response and circadian rhythm by directly visualizing these receptors as a whole brain level. As a preliminary study, we analyzed dynamics of GR using primary cultured neurons obtained from GFP-GR knock-in mice, and furthermore, we observed a fixed brain from GFP-GR knock-in mouse as a whole brain level using a two-photon laser microscope. We succeeded a direct visualization of GFP-GR at the depth of layer II of cerebral cortex of GFP-GR knock-in mouse.
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