Cryopreservation of laboratory fish oocytes

2009 Fiscal Year Final Research Report

• Project/Area Number: 19300147
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kochi University
• Principal Investigator: KASAI Magosaburo Kochi University, 教育研究部自然科学系, 教授 (60152617)
• Co-Investigator(Kenkyū-buntansha): EDASHIGE Keisuke 高知大学, 教育研究部自然科学系, 教授 (30175228) ; KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
• Project Period (FY): 2007 – 2009
• Keywords: 凍結保存 / 魚類卵子 / 水チャンネル / ゼブラフィッシュ / 細胞膜透過性

Research Abstract

For the cryopreservation of fish oocytes, immature oocytes with smaller size and high membrane permeability are suitable. First, we developed a reliable method for in vitro maturation of immature zebrafish oocytes at middle stage III. Then, we showed that exogenous expression of water/cryoprotectant channel (aquaporin 3) in middle stage III oocytes increases the permeability to water and cryoprotectants without decreasing the rates of maturation and development till hatching. However, we did not succeed in the vitrification of oocytes..

Research Products

• [Journal Article] Equilibrium vitrification of mouse embryos (2010)
  • Author(s): Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai
  • Journal Title: Biology of Reproduction 82(2) Pages: 444-450
  • Peer Reviewed
• [Journal Article] Development of a reliable in vitro maturation system for zebrafish oocytes (2008)
  • Author(s): Shinsuke Seki, Toshimitsu Kouya, Ryoma Tsuchiya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige
  • Journal Title: Reproduction 135(3) Pages: 285-292
  • Peer Reviewed
• [Journal Article] Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival (2008)
  • Author(s): Bo Jin, Kenji Kusanagi, Makiko Ueda, Shinsuke Seki, Delgado M. Valdez Jr., Keisuke Edashige, Magosaburo Kasai
  • Journal Title: Cryobiology 56(3) Pages: 233-240
  • Peer Reviewed
• [Journal Article] The mechanism by which mouse spermatozoa are injured during freezing (2008)
  • Author(s): Bo Jin, Chihiro Yamasaki, Naoko Yamada, Shinsuke Seki, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige
  • Journal Title: Journal of Reproduction and Development 54(4) Pages: 265-269
  • Peer Reviewed
• [Journal Article] The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio) (2007)
  • Author(s): Shinsuke Seki, Toshimitsu Kouya, Delgado M. Valdez Jr., Bo Jin, Takao Hara, Naoya Saida, Magosaburo Kasai, Keisuke Edashige
  • Journal Title: Cryobiology 54(1) Pages: 121-124
  • Peer Reviewed
• [Journal Article] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio) (2007)
  • Author(s): Shinsuke Seki, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr., Bo Jin, Magosaburo Kasai, Keisuke Edashige
  • Journal Title: Journal of Reproduction and Development 53(3) Pages: 597-603
  • Peer Reviewed
• [Journal Article] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae (2007)
  • Author(s): Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai
  • Journal Title: Biology of Reproduction 77(2) Pages: 365-375
  • Peer Reviewed
• [Presentation] Equilibrium vitrification of mouse embryos (2009)
  • Author(s): Bo Jin, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Keisuke Edashige, Magosaburo Kasai
  • Organizer: The 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo
  • Year and Date: 20090719-20090723
• [Presentation] Survival of oocytes in antral follicles of vitrified mouse ovaries (2009)
  • Author(s): Keisuke Edashige, Delgado M. Valdez Jr., Hiroshi Honda, Yu Nishikado, Bo Jin, Magosaburo Kasai
  • Organizer: The 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo
  • Year and Date: 20090719-20090723
• [Presentation] Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes-its relevance to their tolerance to cryopreservation- (2009)
  • Author(s): Keisuke Edashige, Bo Jin, Ryu-ichi Higashiyama, Jun-ichi Yonezawa, Masashi Miyake, Sei-ichi Takahashi, Ken-ichi Yazawa, Magosaburo Kasai
  • Organizer: The 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo
  • Year and Date: 20090719-20090723
• [Presentation] Effect of the expression of aquaporins 1 and 3 in mouse MII oocytes on the nucleation temperature for intracellular ice formation (2009)
  • Author(s): Shinsuke Seki, Keisuke Edashige, Peter Mazur
  • Organizer: The 46th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Sapporo
  • Year and Date: 20090719-20090723
• [Presentation] Magosaburo Kasai, Keisuke Edashige. Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio) (2008)
  • Author(s): Shinsuke Seki, Bo Jin, Tsuchiya Ryoma, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr
  • Organizer: The 5th Annual Conference of the Asia Reproductive Biotechnology Society
  • Place of Presentation: Kunming
  • Year and Date: 20081127-20081201
• [Presentation] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III (2008)
  • Author(s): Delgado M. Valdez. Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige
  • Organizer: The 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Charlotte
  • Year and Date: 20080720-20080723
• [Presentation] The pathway for the movement of water and cryoprotectants in bovine oocytes and embryos (2008)
  • Author(s): Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M. Valdez, Jr., Magosaburo Kasai
  • Organizer: The 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Charlotte
  • Year and Date: 20080720-20080723
• [Presentation] Effects of raffinose concentration and methyl-beta-cyclodextrin on IVF with cryopreserved C57BL/6 sperm (2008)
  • Author(s): Keiji Mochida, Chihiro Koshimoto, Keisuke Edashige, Kentaro Aizawa, Kyuichi Taguma, Akihiko Ohta, Atsuo Ogura
  • Organizer: The 45th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Charlotte
  • Year and Date: 20080720-20080723
• [Presentation] Simple technique to improve motility and fertility in frozen C57BL/6J mouse sperm (2007)
  • Author(s): Chihiro Koshimoto, Yuuki Schichi, Keisuke Edashige, Magosaburo Kasai, Keiji Mochida, Akio Shinohara
  • Organizer: The 44th annual Meeting of the Society for Cryobiology
  • Place of Presentation: Lake Louse
  • Year and Date: 20070828-20070801
• [Presentation] ryobiological properties of immature oocytes assessed by the ability to be fertilized and to develop to term (2007)
  • Author(s): Shinsuke Seki, Kouya Toshimitsu, Ryoma Tsuchiya, Delgado M Valdez Jr., Bo Jin, Naoya Saida, Magosaburo Kasai, Keisuke Edashige
  • Organizer: The 44th Annua Meeting of the Society for Cryobiology
  • Place of Presentation: Lake Louse
  • Year and Date: 20070728-20070801
• [Presentation] Assessment of the cryoprotectant permeability and cryoprotectant toxicity of aquaporin 3-expressing immature medaka (Oryzias latipes) oocytes (2007)
  • Author(s): Delgado M. Valdez Jr., Seki Shinsuke, Takao Hara, Naoya Saida, Yu Nishikado, Kasai Magosaburo, Edashige Keisuke
  • Organizer: The 44th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Lake Louse
  • Year and Date: 20070728-20070801
• [Presentation] The role of urea transporters in the movement of cryoprotectants across the plasma membrane in mouse morulae (2007)
  • Author(s): Keisuke Edashige, Satoshi Ohta, Tomomi Kajino, Tatsunaga Kuwano, Bo Jin, Delgado M. Valdez Jr., Shinsuke Seki, Magosaburo Kasai
  • Organizer: The 44th Annual Meeting of the Society for Cryobiology
  • Place of Presentation: Lake Louse
  • Year and Date: 20070728-20070801
• [Presentation] The role of aquaporin-3 in the movement of water and cryoprotectants in mouse morulae (2007)
  • Author(s): Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo. Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai
  • Organizer: The 5th International Conference of Aquaporin
  • Place of Presentation: Nara
  • Year and Date: 20070713-20070716
• [Presentation] Intracellular ice formation in vitrified mouse morulae during warming (2007)
  • Author(s): Bo Jin, Kenji Kusanagi, Makiko Ueda, Keisuke Edashige, Magosaburo Kasai
  • Organizer: The 33rd Annual Conference of the International Embryo Transfer Society
  • Place of Presentation: Kyoto
  • Year and Date: 20070107-20070109
• [Book] Movement of water and cryoprotectants in mouse oocytes and embryos at different stages: relevance to cryopreservation (2010)
  • Author(s): Magosaburo Kasai, Keisuke Edashige
  • Total Pages: 16-23
  • Publisher: Cambridge University Press
• [Book] Vitrification in animal reproduction: vitrification of embryos using conventional straws with an ethylene glycol-based solutions (2007)
  • Author(s): Magosaburo Kasai, Keisuke Edashige
  • Total Pages: 75-85
  • Publisher: Informa Healthcare, London