2009 Fiscal Year Final Research Report
Development of diagnostic methods for Parkinson disease using high fieled magnetic resonance imaging
| Project/Area Number |
19300158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TOOYAMA Ikuo Shiga University of Medical Science, 分子神経科学研究センター, 教授 (20207533)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Hiroyasu 滋賀医科大学, 分子神経科学研究センター, 特任教授 (90102912)
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| Co-Investigator(Renkei-kenkyūsha) |
INUBUSHI Toshiro 滋賀医科大学, MR医学総合研究センター, 教授 (20213142)
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| Project Period (FY) |
2007 – 2009
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| Keywords | パーキンソン病 / 核磁気共鳴 / 診断薬 / フッ素MR画像法 |
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| Research Abstract |
We examined the feasibility of high filed MRI, particularly ^<19>F-MRI, for the diagnosis of Parkinson disease using PC12 cells and 6OH-dopamine treated rats. We measured ^<19>F-NMR signals in the homogenates of rat brain and several agents such as 6-^<19>F-fluoro-L-DOPA (^<19>F-DOPA) and chemicals that are involved in the activity of dopamine neurons. Among them, ^<19>F-DOPA showed the best signal to nose ratio. When ^<19>F-DOPA was added into culture medium of PC12 cells, MR images of ^<19>F-DOPA in about 50 mg of PC12 cells were successfully detected. When we measured the brain from 6-OH-dopamine treated rats that received intra-peritoneal injection of ^<19>F-DOPA, MR images of ^<19>F-DOPA in the striatum were successfully detected by 10 hours measurements of 7-tesla MRI. This study gives us the fundamental data for the development of diagnostic methods for Parkinson disease using high fieled MRI.
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[Journal Article] Oxidative stress induction of DJ-1 protein in reactive astrocytes scavenges free radicals and reduces cell injury.2009
Author(s)
Yanagida T, Tsushima J, Kitamura Y, Yanagisawa D, Takata T, Shibaike T, Yamamoto A, Taniguchi T, Yasui H, Morikawa S, Inubiushi T, Tooyama I, Agira H
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Journal Title
Oxid Med Cell Longev. 2
Pages: 36-42
Peer Reviewed
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[Journal Article] Trifluoromethoxy-benzylated ligands improve amyloid detection in the brain using 19F magnetic resonance imaging2009
Author(s)
Amatsubo T, Morikawa S, Matsuda K, Inubushi T, Urushitani M, Taguchi H, Shirai N, Hirao K, Kato M, Morino K, Kimura H, Nakano I, Yoshida C, Okada T, Sano M, Tooyama I
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Journal Title
Neurosci Res 63
Pages: 76-81
Peer Reviewed
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[Presentation] Interaction of the brain tissues with trifluoromethoxy-benzylated ligands for amyloid detection using 19F magnetic resonance imaging2008
Author(s)
Tomone Amatsubo, Shigehiro Morikawa, Keiko Matsuda, Toshiro Inubushi, Makoto Urushitani, Hiroyasu Taguchi, Nobuaki Shirai, Koichi Hirao, Masanari Kato, Kyuya Morino, Hirohiko Kimura, Ichiro Nakano, Chikako Yoshida, Takashi Okada, Mitsuo Sano, Ikuo Tooyama
Organizer
第10回国際アルツハイマー病会議(ICAD2008)
Place of Presentation
米国シカゴ市
Year and Date
20080726-20080801
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