2009 Fiscal Year Final Research Report
Development of a intraoperative monitoring system for myocardial metabolism using autofluorescence of NADH and FAD
| Project/Area Number |
19300166
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
OGASAWARA Yasuo Kawasaki Medical School, 医学部, 准教授 (10152365)
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| Co-Investigator(Kenkyū-buntansha) |
YADA Toyotaka 川崎医科大学, 医学部, 講師 (00210279)
NAKAMOTO Hiroshi 川崎医科大学, 医学部, 助教 (10299183)
OKABAYASHI Hitoshi 岩手医科大学, 医学部, 教授 (10204009)
FUKUHIRO Yoshiaki 岩手医科大学, 医学部, 助教 (20228927)
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| Research Collaborator |
HASEGAWA Katsuya 宇宙航空研究開発機構, 宇宙科学研究本部技術開発部, 開発員
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| Project Period (FY) |
2007 – 2009
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| Keywords | NADH / FAD / 蛍光 / 心筋代謝 / 二波長励起 |
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| Research Abstract |
It is highly important to observe changes in oxygen concentration on the myocardial surface during a cardiac surgery. Both flavin adenine dinucleotide (FAD) and reduced nicotinamide adenine dinucleotide (NADH) in myocardial cells emit auto-fluorescence. An increase in oxygen tension causes enhanced FAD level as well as attenuated NADH level. We thus measured both FAD and NADH fluorescences using a newly developed fluorescence imaging system with a dual-wavelength excitation source and obtained the ratio of FAD/NADH to evaluate a change in the myocardial metabolism. The myocardial surface of experimental animals was observed by the system. We confirmed the applicability of the system to evaluation of the cardiac metabolism.
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