2009 Fiscal Year Final Research Report
Development of intravital microscope for measurement of capillary and coronary blood flow, and coronary microvascular disturbance of diabetes mellitus and circulatory reconstruction
| Project/Area Number |
19300167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
YADA Toyotaka Kawasaki Medical School, 医学部, 講師 (00210279)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMOTO Hiroshi 川崎医科大学, 医学部, 助教 (10299183)
MORITA Yoshitaka 川崎医科大学, 医学部, 講師 (50346441)
OGASAWARA Yasuo 川崎医科大学, 医学部, 准教授 (10152365)
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| Co-Investigator(Renkei-kenkyūsha) |
KAJIYA Fumihiko 川崎医療福祉大学, 医療技術学部, 教授 (70029114)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 医用 / 生体画像 |
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| Research Abstract |
It was possible to visualize the blood capillary and observe the coronary microcirculation in dogs of diabetes mellitus by a CCD intravital microscope of high accuracy. We have demonstrated that endothelium-derived hyperpolarizing factors (EDHF)/H_2O_2 and NO were key mediators of vasodilatation of coronary native collateral microvassels after myocardial ischemia, and erythropoietin (EPO) and angiotensin receptor blockers (ARB) enhanced the vasodilatation in canine coronary collateral microvessels in vivo, and also enhanced H_2O_2-induced canine coronary collateral vasodilatation. Myocardial expression of eNOS and Akt activity as the ratio of phospho-Akt/total Akt in the myocardium of ischemic LAD area of control group were increased by EPO. These results indicate that EPO improves eNOS protein expression and Akt phosphorylation, at least in part, through activation of PI3K/Akt and EDHF/H_2O_2 pathway. EPO, ARB and EDHF/H_2O_2 play an important role for improvement factor of vascular endothelial disturbance.
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[Journal Article] A Specific Role for eNOS-Derived Reactive Oxygen Species in Atherosclerosis Progression2007
Author(s)
Tomofumi Takaya, Ken-ichi Hirata, Tomoya Yamashita, Masakazu Shinohara, Naoto Sasaki, Nobutaka Inoue, Toyotaka Yada, 以下省略(7名)
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Journal Title
Arteriosclerosis, Thrombosis, and Vascular Biology Vol.27,No.7
Pages: 1632-1637
Peer Reviewed
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[Journal Article] Impaired NO-mediated vasodilation with increased superoxide but robust EDHF function in right ventricular arterial microvessels of pulmonary hypertensive rats2007
Author(s)
Masahito Kajiya, Masanori Hirota, Yousuke Inai, Takahiko Kiyooka, Taro Morimoto, Tatsuo Iwasaki, Kousuke Endo, Satoshi Mohri, Juichiro Shimizu, Toyotaka Yada, 以下省略(4名)
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Journal Title
Am J Physiol Heart Circ Physiol Vol.292,No.6
Pages: H2737-H2744
Peer Reviewed
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[Presentation] Cardioprotective effects of hydrogen peroxide and erythropoietin during acute coronary occlusion in canine coronary native collateral microcirculation in vivo2010
Author(s)
Toyotaka Yada, Hiroaki Shimokawa, Osamu Hiramatsu, Minoru Satoh, Naoki Kashihara, Yoshiro Shinozaki, Hidezo Mori, Aya Takaki, Masami Goto, Yasuo Ogasawara, Fumihiko Kajiya
Organizer
Circulation Journal
Place of Presentation
第74回日本循環器学会総会、京都
Year and Date
20100000
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[Presentation] Cardioprotective effects of hydrogen peroxide and erythropoietin during acute coronary occlusion in canine coronary native collateral microvessels in vivo2009
Author(s)
Toyotaka Yada, Hiroaki Shimokawa, Osamu Hiramatsu, Yoshiro Shinozaki, Hidezo Mori, Aya Takaki, Masami Goto, Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
Organizer
American Heart Association, Scientific Sessions 2009
Place of Presentation
Orlando, Florida, USA
Year and Date
20090000
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[Presentation] CAPD療法における一酸化窒素(NO)とテトラヒドロビオプテリン(BH4)の動態2009
Author(s)
佐々木環, 望月精一, 矢田豊隆, 高山綾, 藤本壮八, 堀家英之, 佐藤稔, 十倉健彦, 駒井則夫, 冨田奈留也, 柏原直樹
Organizer
日本腎臓学会誌, 第52回日本腎臓学会学術総会
Place of Presentation
横浜
Year and Date
20090000
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[Presentation] AngiotensinIIType 1Receptor Blocker Ameliorates Uncoupled Endothelial Nitric Oxide Synthase in Rats with Streptozotocine Induced Diabetic Nephropathy、Journal of American2008
Author(s)
Minoru Satoh, Sohachi Fujimoto, Yoshisuke Haruna, Toyotaka Yada, Atsunori Kuwabara, Hajime Nagasu, Daisuke Yorimitu, Tamehachi Namikoshi, Naruya Tomita, Tamaki Sasaki, Naoki Kashihara
Organizer
American Society of Nephrology 41st Annual Meeting & Scientific Exposition
Place of Presentation
Philadelphia, Pennsylvania, USA
Year and Date
20080000
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[Presentation] Crucial role of nitric oxide synthases system in endothelium-dependent hyperpolarization in mice、Circulation2008
Author(s)
Aya Takaki, Keiko Morikawa, Yoshinori Murayama, Ender Tekes, Hiroto Yamagishi, Junko Ohashi, Masato Tsutsui, Toyotaka Yada, Nobuyuki Yanagihara, Hiroaki Shimokawa
Organizer
American Heart Association, Scientific Sessions 2008
Place of Presentation
New Orleans, USA
Year and Date
20080000
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[Presentation] 腹膜透析(PD)+血液透析(HD)療法における腹腔内NO代謝と酸化ストレス2008
Author(s)
藤本壮八, 佐々木環, 堀家英之, 望月精一, 高山綾, 矢田豊隆, 十倉健彦, 佐藤稔, 駒井則夫, 冨田奈留也, 柏原直樹
Organizer
日本透析医学会雑誌, 第53回日本透析医学会学術集会
Place of Presentation
神戸
Year and Date
20080000
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[Presentation] CAPD透析液中のNO代謝関連物質の動態評価2008
Author(s)
藤本壮八, 佐々木環, 堀家英之, 十倉健彦, 佐藤稔, 駒井則夫, 冨田奈留也, 柏原直樹, 望月精一, 高山綾, 矢田豊隆
Organizer
日本腎臓学会誌, 第51回日本腎臓学会学術総会
Place of Presentation
福岡
Year and Date
20080000
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[Presentation] Hydrogen peroxide plays a crucial role in coronary microvascular vasodilation with increased oxidative stress in right ventricles of pulmonary hypertensive rats、Circulation2007
Author(s)
Masahito Kajiya, Yousuke Inai, Taro Morimoto, Tatsuo Iwasaki, Kousuke Endo, Satoshi Mohri, Toyotaka Yada, Keiji Naruse, Tohru Ohe, Fumihiko Kajiya
Organizer
American Heart Association, Scientific Sessions 2007
Place of Presentation
Orlando, Florida, USA
Year and Date
20070000
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[Presentation] Crucial role of Cu/Zn-SOD in the synthesis of endothelium-derived hyperpolarizing factor(EDHF) during reactive hyperemia in mouse mesenteric microcirculation in vivo、Circulation2007
Author(s)
Toyotaka Yada, Hiroaki Shimokawa, Osamu Hiramatsu, Keiko Morikawa, Aya Takaki, Yoshiro Shinozaki, Hidezo Mori, Masami Goto, Yasuo Ogasawara, Fumihiko Kajiya
Organizer
American Heart Association, Scientific Sessions 2007
Place of Presentation
Orlando, Florida, USA
Year and Date
20070000
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[Presentation] Protective role of hydrogen peroxide and erythropoietin during acute coronary occlusion/reperfusion in native coronary collateral circulation in dogs in vivo、Circulation2007
Author(s)
Toyotaka Yada, Hiroaki Shimokawa, Osamu Hiramatsu, Yoshiro Shinozaki, Hidezo Mori, Masami Goto, Yasuo Ogasawara, Fumihiko Kajiya
Organizer
American Heart Association, Scientific Sessions 2007
Place of Presentation
Orlando, Florida, USA
Year and Date
20070000
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