Evaluation of Angiogenesis by High Resolution Three-dimensional Ultrasound Molecular Imaging

2009 Fiscal Year Final Research Report

• Project/Area Number: 19300179
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Tohoku University
• Principal Investigator: SAIJO Yoshifumi Tohoku University, 大学院・医工学研究科, 教授 (00292277)
• Co-Investigator(Kenkyū-buntansha): SATO Yasufumi 東北大学, 加齢医学研究所, 教授 (50178779) ; KODAMA Tetsuya 東北大学, 大学院・医工学研究科, 教授 (40271986)
• Project Period (FY): 2007 – 2009
• Keywords: 超音波医科学

Research Abstract

High resolution three-dimensional ultrasound molecular imaging was developed for evaluation of angiogenesis in small animals such as mice. The following were achieved ; 1. Three-dimensional ultrasound imaging system with the frequency range up to 150 MHz and the spatial resolution of 10 micron, 2. Image analysis algorithm for classification of vessels and surrounding tissues by correlation of RF signals in the consecutive frames, 3. Portable scanner for easy and repetitive use, 4. Molecular imaging of angiogenesis with labeled nano-bubble.

Research Products

• [Journal Article] Acoustic microscopy : latest developments and applications (2009)
  • Author(s): Saijo Y.
  • Journal Title: Imaging in Medicine Vol. 1, No. 1 Pages: 47-63
  • Peer Reviewed
• [Journal Article] Changes of articular cartilage after immobilization in a rat knee contracture model (2009)
  • Author(s): Hagiwara Y, Ando A, Chimoto E, Saijo Y, Ohmori-Matsuda K, Itoi E.
  • Journal Title: J Orthop Res. Vol. 27, No. 2 Pages: 236-242
  • Peer Reviewed
• [Journal Article] Ultrasonic intensity microscopy for imaging of living cells (2009)
  • Author(s): Hagiwara Y, Saijo Y, Ando A, Chimoto E, Suda H, Onoda Y, Itoi E.
  • Journal Title: Ultrasonics Vol. 49, No. 3 Pages: 386-388
  • Peer Reviewed
• [Journal Article] Degenerated coracoacromial ligament in shoulders with rotator cuff tears shows higher elastic modulus : measurement with scanning acoustic microscopy (2009)
  • Author(s): Kijima H, Minagawa H, Saijo Y, Sano H, Tomioka T, Yamamoto N, Shimada Y, Okada K, Itoi E.
  • Journal Title: J Orthop Sci. Vol. 14, No. 1 Pages: 62-7
  • Peer Reviewed
• [Journal Article] Fractal dimension of 40MHz intravascular ultrasound radio frequency signals (2008)
  • Author(s): Santos Filho E, Saijo Y, Tanaka A, Yambe T, Yoshizawa M.
  • Journal Title: Ultrasonics Vol. 48, No. 1 Pages: 35-39
  • Peer Reviewed
• [Journal Article] Detection and quantification of calcifications in intravascular ultrasound images by automatic thresholding (2008)
  • Author(s): Santos Filho E, Saijo Y, Tanaka A, Yoshizawa M.
  • Journal Title: Ultrasound Med Biol. Vol. 34, No. 1 Pages: 160-165
  • Peer Reviewed
• [Journal Article] High frequency ultrasound imaging of surface and subsurface structures of fingerprints (2008)
  • Author(s): Saijo Y, Kobayashi K, Okada N, Hozumi N, Hagiwara Y, Tanaka A, Iwamoto T.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. 2008 Pages: 2173-2176
  • Peer Reviewed
• [Journal Article] High frequency ultrasound characterization of artificial skin (2008)
  • Author(s): Iwamoto T, Saijo Y, Hozumi N, Kobayashi K, Okada N, Tanaka A, Yoshizawa M.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. 2008 Pages: 2185-2188
  • Peer Reviewed
• [Journal Article] Elasticity of the supraspinatus tendon-muscle unit is preserved after acute tendon tearing in the rabbit (2008)
  • Author(s): Mineta M, Sano H, Ichinose R, Saijo Y, Itoi E.
  • Journal Title: Tohoku J Exp Med. Vol. 216, No. 1 Pages: 17-24
  • Peer Reviewed
• [Journal Article] Ultrasonic tissue characterization of atherosclerosis by a speed-of-sound microscanning system (2007)
  • Author(s): Saijo Y, Hozumi N, Kobayashi K, OkadaN, Santos Filho ED, Sasaki H, Yambe Tanaka M.
  • Journal Title: IEEE Trans Ultrason Ferroelectr Freq Control Vol. 54, No. 8 Pages: 1571-1577
  • Peer Reviewed
• [Journal Article] Measurement of soft tissue elasticity in the congenital clubfoot using scanning acoustic microscope (2007)
  • Author(s): Hattori K, Sano H, Saijo Y, Kita A, Hatori M, Kokubun S, Itoi E.
  • Journal Title: J Pediatr Orthop B. Vol. 16, No. 5 Pages: 357-362
  • Peer Reviewed
• [Journal Article] Blood flow visualization of left atrial spontaneous echo contrast (SEC) using gradient based optical flow estimation (2007)
  • Author(s): Tanaka A, Saijo Y.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. Vol. 1 Pages: 4500-3
  • Peer Reviewed
• [Journal Article] Morphological approach for the functional improvement of an artificial myocardial assist device using shape memory alloy fibres (2007)
  • Author(s): Shiraishi Y, Yambe T, Saijo Y, Sato F, Tanaka A, Yoshizawa M, Ogawa D, Wada Y, Itoh S, Sakata R, Park Y, Uematsu M, Umezu M, Fujimoto T, Masumoto N, Liu H, Baba A, Konno S, Nitta S, Imachi K, Tabayashi K, Sasada H, Homma D.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. Vol. 1 Pages: 3974-7
  • Peer Reviewed
• [Journal Article] Ultrasound speed and impedance microscopy for in vivo imaging (2007)
  • Author(s): Saijo Y, Hozumi N, Kobayashi K, Okada N, Ishiguro T, Hagiwara Y, Dos Santos Filho E, Yambe T.
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. Vol. 1 Pages: 1350-3
  • Peer Reviewed
• [Journal Article] Biochemical evaluation of an artificial anal sphincter made from shape memory alloys (2007)
  • Author(s): Liu H, Luo Y, Higa M, Zhang X, Saijo Y, Shiraishi Y, Sekine K, Yambe T.
  • Journal Title: J Artif Organs. Vol. 10 Pages: 223-227
  • Peer Reviewed
• [Journal Article] Macrophage colony-stimulating factor improves cardiac function after ischemic injury by inducing vascular endothelial growth factor production and survival of cardiomyocytes (2007)
  • Author(s): Okazaki T, Ebihara S, Asada M, Yamanda S, Saijo Y, Shiraishi Y, Ebihara T, Niu K, Mei H, Arai H, Yambe T.
  • Journal Title: Am J Pathol. Vol. 171, No. 4 Pages: 1093-1103
  • Peer Reviewed
• [Journal Article] パラメトリックIVUSの臨床応用 (2007)
  • Author(s): 西條芳文
  • Journal Title: Jap J Cardiovasc Cathet Therap. Vol. 7, No. 2 Pages: 131-139
  • Peer Reviewed
• [Presentation] Acoustic microscopy-Beyond high resolution imaging (2009)
  • Author(s): Saijo Y.
  • Organizer: Invited Lecture at the 2009 IEEE International Ultrasonics Symposium
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-09-21
• [Presentation] Biomedical applications of acoustic microscopy (2009)
  • Author(s): Saijo Y.
  • Organizer: Tutorial course at the 2009 IEEE International Ultrasonics Symposium
  • Place of Presentation: Rome, Italy
  • Year and Date: 2009-09-19
• [Presentation] Visualization of microstructure of the skin by 3D ultrasound microscope (2009)
  • Author(s): Saijo Y, Kobayashi K, Hozumi N, Tanaka A.
  • Organizer: 12th World Congress of the World Federation for Ultrasound in Medicine and Biology
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2009-08-30
• [Presentation] Multimode ultrasound microscope for biomedical applications (2009)
  • Author(s): Saijo Y.
  • Organizer: Categorical course at the 2009 American Institute of Ultrasound in Medicine Annual Convention
  • Place of Presentation: New York, NY, USA
  • Year and Date: 2009-04-04
• [Presentation] Visualization of microvessels in skin by three-dimensional ultrasound microscope (2009)
  • Author(s): Saijo Y, Kobayashi K, Hozumi N, Tanaka A, Sakai S.
  • Organizer: 30th International Symposium on Acoustical Imaging
  • Place of Presentation: Monterey, USA
  • Year and Date: 2009-03-02
• [Presentation] Biomedical Application of acoustic microscopy-Diagnosis, assessing echogenecity and biomechanics (2008)
  • Author(s): Saijo Y, Hagiwara Y, Kobayashi K, Okada N, Tanaka A, Hozumi N, Tanaka M.
  • Organizer: 2008 IEEE International Ultrasonics Symposium
  • Place of Presentation: Beijing, China
  • Year and Date: 2008-11-04
• [Presentation] Visualization of surface and 3D morphology of skin by 100MHz ultrasound (2008)
  • Author(s): Saijo Y
  • Organizer: 6th International Conference on Ultrasonic Biomedical Microscanning
  • Place of Presentation: Malibu, CA, USA
  • Year and Date: 2008-09-24
• [Presentation] Recent progress of acoustic microscopy for medicine and biology (2008)
  • Author(s): Saijo Y
  • Organizer: 155th Meeting of the Acoustical Society of America integrated with 5th Forum Acusticum of the EAA, 2nd ASA-EAA Joint Conference
  • Place of Presentation: Paris, France
  • Year and Date: 2008-07-01
• [Presentation] Ultra-mobile echo network in health care system (2008)
  • Author(s): Saijo Y, Iwamoto T, Kobayashi K, Yamaguchi S, Tsunoda H, Nakayama H, Kato N, Nemoto Y.
  • Organizer: 21st IEEE International Symposium on Computer-Based Medical Systems
  • Place of Presentation: Jyvaskyla, Finland
  • Year and Date: 2008-06-18
• [Presentation] Three-dimensional ultrasound imaging of regenerated skin with high frequency ultrasound (2008)
  • Author(s): Saijo Y, Hagiwara Y, Kobayashi K, Okada N, Hozumi N, Tanaka A, Iwamoto T.
  • Organizer: 5th IEEE International Symposium on Biomedical Imaging
  • Place of Presentation: Paris, France
  • Year and Date: 2008-05-15
• [Presentation] 皮膚組織の3次元超音波顕微鏡像 (2008)
  • Author(s): 西條芳文, 岩本貴宏, 小林和人, 岡田長也, 萩原嘉廣, 田中明, 穂積直裕
  • Organizer: 第35回日本超音波医学会東北地方会
  • Place of Presentation: 仙台
  • Year and Date: 2008-03-23
• [Presentation] Precise calibration for biological acoustic impedance microscope (2007)
  • Author(s): Hozumi N, Nakao A, Terauchi S, Nagao M, Yoshida S, Kobayashi K, Yamamoto S, Saijo Y.
  • Organizer: 2007 IEEE International Ultrasonics Sysmposium
  • Place of Presentation: New York, USA
  • Year and Date: 2007-10-30
• [Presentation] B-mode and C-mode imaging of regenerated 3D skin model with 100MHz ultrasound (2007)
  • Author(s): Saijo Y, Hagiwara Y, Kobayashi K, Okada N, Tanaka A, Hozumi N, Tomihata K.
  • Organizer: 2007 IEEE International Ultrasonics Symposium
  • Place of Presentation: New York, USA
  • Year and Date: 2007-10-29
• [Presentation] オプティカルフロー法による心血管内血流の可視化 (2007)
  • Author(s): 西條芳文, 田中明, 田林晄一, 山家智之
  • Organizer: 第55回日本心臓病学会
  • Place of Presentation: 千葉
  • Year and Date: 2007-09-11
• [Presentation] Ultrasound Speed and Impedance Microscopy for in vivo Imaging (2007)
  • Author(s): Saijo Y, Hozumi N, Kobayashi K, Okada N, Ishiguro T, Hagiwara Y, Dos Santos Filho E, Yamabe T.
  • Organizer: 29th IEEE EMBS Annual International Conference
  • Place of Presentation: Lyon, France
  • Year and Date: 2007-08-23
• [Presentation] 生体内超音波ナノ・イメージング法による線維芽細胞の可視化 (2007)
  • Author(s): 西條芳文, 穂積直裕, 小林和人, Santos Filho Esmeraldo
  • Organizer: 日本超音波医学会第80回学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2007-05-19
• [Book] Ultrasonic nano-imaging system for medicine and biology.(Iwaki Akiyama ed.)Acoustical Imaging Vol 29 (2008)
  • Author(s): Saijo Y, Hozumi N, Kobayashi K, Okada N, Hagiwara Y, Sasaki H, Santos Filho E, Yambe T.
  • Total Pages: 181-186
  • Publisher: Springer
• [Book] Ultrasonic measurement of micro-acoustic properties of the biological soft materials.(Tribikram Kundu ed.)Advanced Ultrasonic Methods for Material Characterization (2007)
  • Author(s): Saijo Y.
  • Total Pages: 89-113
  • Publisher: ISTE
• [Book] 第2章. 第4節. 4. 超音波顕微鏡による組織性状の可視化.(小川誠二、上野照剛 編集)非侵襲・可視化技術ハンドブック (2007)
  • Author(s): 西條芳文
  • Total Pages: 241-249
  • Publisher: 株式会社エヌティーエス
• [Remarks] ホームページ等
  • URL: http://biomedimg.web.officelive.com/default.aspx