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2009 Fiscal Year Final Research Report

Regulation of translesion DNA synthesis by post-translational modifications

Research Project

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Project/Area Number 19310036
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionOsaka University

Principal Investigator

MASUTANI Chikahide  Osaka University, 大学院・生命機能研究科, 准教授 (40241252)

Project Period (FY) 2007 – 2009
Keywords損傷乗り越えDNA複製 / DNA複製 / 翻訳後修飾
Research Abstract

Human DNA polymerase eta (Polη) is the responsible gene product of xeroderma pigmentosum variant, a cancer-prone genetic disorder, and protects cells from cell death or carcinogenesis by catalyzing accurate translesion DNA synthesis (TLS). During this project, we showed that Polη has mutagenic properties depending on DNA damages and situations. We have also identified novel protein-protein interactions and post-translational modifications of Polη and related proteins which could be important for regulating TLS in human cells.

  • Research Products

    (27 results)

All 2010 2009 2008 2007 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (11 results) Book (2 results) Remarks (1 results)

  • [Journal Article] The molecular chaperone Hsp90 regulates accumulation of DNA polymerase η at replication stalling sites in UV-irradiated cells2010

    • Author(s)
      Sekimoto, T., Oda, T., Pozo, F.M., Murakumo, Y., Masutani, C., Hanaoka, F., Yamashita, T
    • Journal Title

      Mol. Cell 37

      Pages: 79-89

    • Peer Reviewed
  • [Journal Article] Polymerization by DNA polymerase η is blocked by cis-diamminedichloroplatinum(II) 1, 3-d(GpTpG) crosslink: Implications for cytotoxic effects in nucleotide excision repair-negative tumor cells2010

    • Author(s)
      Chijiwa, S., Masutani, C., Hanaoka, F., Iwai, S., Kuraoka, I
    • Journal Title

      Carcinogenesis 31

      Pages: 388-393

    • Peer Reviewed
  • [Journal Article] Critical amino acids involved in erroneous incorporation of oxidized nucleotides by human DNA polymerase η and κ2010

    • Author(s)
      Katafuchi, A., Sassa, A., Niimi, N., Gruz, P., Fujimoto, H., Masutani, C., Hanaoka, F., Ohta, T., Nohmi, T
    • Journal Title

      Nucleic Acids Res 38

      Pages: 859-867

    • Peer Reviewed
  • [Journal Article] A novel interaction between human DNA polymerase η and MutLα2009

    • Author(s)
      Kanao, R., Hanaoka, F., Masutani, C
    • Journal Title

      Biochem. Biophys. Res. Commun 389

      Pages: 40-45

    • Peer Reviewed
  • [Journal Article] Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP) in vitro: REV1 inserts dC opposite the lesion and DNA polymerase κ potentially catalyzes extension reaction from the 3'-dC terminus2009

    • Author(s)
      Fukuda, H., Takamura-Enya, T., Masuda, Y., Nohmi, T., Seki, C., Kamiya, K., Sugimura, T., Masutani, C., Hanaoka, F., Nakagama, H
    • Journal Title

      J. Biol. Chem 284

      Pages: 25585-25592

    • Peer Reviewed
  • [Journal Article] Interaction with DNA polymerase η is required for nuclear accumulation of REV1 and suppression of spontaneous mutations in human cells2009

    • Author(s)
      Akagi, J., Masutani, C., Kataoka, Y., Kan, T., Ohashi, E., Mori, T., Ohmori, H., Hanaoka, F
    • Journal Title

      DNA Repair 8

      Pages: 585-599

    • Peer Reviewed
  • [Journal Article] Penicilliols A and B, novel inhibitors specific to mammalian Y-family DNA polymerases2009

    • Author(s)
      Kimura, T., Takeuchi, T., Kumamoto-Yonezawa, Y., Ohashi, E., Ohmori, H., Masutani, C., Hanaoka, F., Sugawara, F., Yoshida, H., Mizushina, Y
    • Journal Title

      Bioorganic Medicinal Chemistry 17

      Pages: 1811-1816

    • Peer Reviewed
  • [Journal Article] Miscoding property of 2'-deoxyinosine, a nitric oxide-derived DNA adduct, during translesion synthesis catalyzed by human DNA polymerases2008

    • Author(s)
      Yasui, M., Suenaga, E., Koyama, N., Masutani, C., Hanaoka, F., Gruz, P., Shibutani, S., Nohmi, T., Hayashi, M., Honma, M
    • Journal Title

      J. Mol. Biol 377

      Pages: 1015-1023

    • Peer Reviewed
  • [Journal Article] Specificity of mutations induced by incorporation of oxidized dNTPs into DNA by human DNA polymeraseη2008

    • Author(s)
      Hidaka, K., Yamada, M., Kamiya, H., Masutani, C., Harashima, H., Hanaoka, F., Nohmi, T
    • Journal Title

      DNA Repair 7

      Pages: 497-506

    • Peer Reviewed
  • [Journal Article] A second PCNA loader complex, Ctf18-RFC, specifically stimulates DNA polymerase η activity2007

    • Author(s)
      Shiomi, Y., Masutani, C., Hanaoka, F., Kimura, H., Tsurimoto, T
    • Journal Title

      J. Biol. Chem 282

      Pages: 20906-20914

    • Peer Reviewed
  • [Journal Article] 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A. T-> C. G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract2007

    • Author(s)
      Satou, K., Kasai, H., Masutani, C., Hanaoka, F., Harashima, H., Kamiya, H
    • Journal Title

      Biochemistry 46

      Pages: 6639-6646

    • Peer Reviewed
  • [Journal Article] DNA polymerase η and θ function in the same genetic pathway to generate mutations at A/T during somatic hypermutation of Ig genes2007

    • Author(s)
      Masuda, K., Ouchida, R., Hikida, M., Kurosaki, T., Yokoi, M., Masutani, C., Seki, M., Wood, R.D., Hanaoka, F., O-Wang, J
    • Journal Title

      J. Biol. Chem 282

      Pages: 17387-17394

    • Peer Reviewed
  • [Journal Article] Efficient and erroneous incorporation of oxidized DNA precursors by human DNA polymerase η2007

    • Author(s)
      Shimizu, M., Gruz, P., Kamiya, H., Masutani, C., Xu, Y., Sugiyama, H., Harashima, H., Hanaoka, F., Nohmi, T
    • Journal Title

      Biochemistry 46

      Pages: 5515-5522

    • Peer Reviewed
  • [Presentation] Analysis of regulatory mechanisms of human pol eta, the XPV gene product2009

    • Author(s)
      益谷央豪
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
  • [Presentation] Physiological relevance of post-translational modifications of PCNA at lysine-164 in human cells2009

    • Author(s)
      金尾梨絵、出口沙織、湯本真弓、花岡文雄、益谷央豪
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
  • [Presentation] The molecular chaperone Hsp90 promotes DNA damage-induced nuclear focus formation of Y-family DNA polymerase REV12009

    • Author(s)
      Franklin Mayca Pozo、小野司、関本隆志、益谷央豪、花岡文雄、村雲芳樹、山下孝之
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
  • [Presentation] Human REV1 is phosphorylated and accumulated at spindle poles in mitotic cells2009

    • Author(s)
      赤木純一、片岡裕貴、益谷央豪、花岡文雄
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
  • [Presentation] Penicilliols A and B, novel inhibitors specific to mammalian Y-family DNA polymerases2009

    • Author(s)
      水品善之、木村拓馬、竹内倫文、米澤裕子、大橋英治、大森治夫、益谷央豪、花岡文雄、吉田弘美、菅原二三男、坂口健吾
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
  • [Presentation] DNAポリメラーゼηのリン酸化による機能制御の解析2008

    • Author(s)
      小野香苗、花岡文雄、益谷央豪
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      20081209-20081212
  • [Presentation] PCNAのモノユビキチン化を制御するメカニズムの探索2008

    • Author(s)
      徐菲、花岡文雄、益谷央豪
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
    • Place of Presentation
      神戸
    • Year and Date
      20081209-20081212
  • [Presentation] Physiological relevance of the Pol eta-REV1 interaction in human cells2008

    • Author(s)
      Masutani, C., Akagi, J., Kataoka, Y., Ohashi, E., Iwai, S., Ohmori, H., Hanaoka, F
    • Organizer
      International Symposium on Chromosome Dynamics in Ise
    • Place of Presentation
      Toba, Japan
    • Year and Date
      20080528-20080530
  • [Presentation] Physiological relevance of the Pol eta-Rev1 interaction in human cells2008

    • Author(s)
      Masutani, C., Akagi, J., Kataoka, Y., Ohashi, E., Iwai, S., Ohmori, H., Hanaoka, F
    • Organizer
      The International Ataxia-telangiectasia Workshop 2008 (ATW2008)
    • Place of Presentation
      Ohtsu, Japan
    • Year and Date
      20080422-20080426
  • [Presentation] 蛋白質間相互作用と翻訳後修飾による損傷乗り越えDNA複製の制御2008

    • Author(s)
      益谷央豪
    • Organizer
      BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
  • [Presentation] 損傷乗り越えDNA複製におけるタンパク質間相互作用2008

    • Author(s)
      益谷央豪、花岡文雄
    • Organizer
      第67回日本癌学会学術総会シンポジウム
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-29
  • [Book] Xeroderma Pigmentosum Variant, XP-V: its product and biological roles2008

    • Author(s)
      Masutani, C., Hanaoka, F., Ahmad, S. I
    • Total Pages
      93-102
    • Publisher
      Adv. Exp. Med. Biol
  • [Book] Methods Mol. Biol(Use of RNAi in C. elegans)2008

    • Author(s)
      Ohkumo, T., Masutani, C., Eki, T., Hanaoka, F
    • Total Pages
      129-137
  • [Remarks]

    • URL

      http://www.fbs.osaka-u.ac.jp/jp/labo/091/

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Published: 2011-06-18   Modified: 2016-04-21  

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