CONFORMATIONAL CHANGE OF NUCLEAR ARCHITECTURE IN RESPONSE TO IONIZING IRRADIATION

2009 Fiscal Year Final Research Report

• Project/Area Number: 19310037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Hiroshima University
• Principal Investigator: TASHIRO Satoshi Hiroshima University, 原爆放射線医科学研究所, 教授 (20243610)
• Co-Investigator(Kenkyū-buntansha): SUN Jying 広島大学, 原爆放射線医科学研究所, 助教 (80397926)
• Project Period (FY): 2007 – 2009
• Keywords: 放射線応答 / ゲノム修復 / クロマチン構造 / 細胞核高次構造

Research Abstract

We examined the mechanism of human cellular response against ionizing irradiation. For this purpose, we analyzed the structure and dynamics of the radiation-induced nuclear foci and reorganization of damaged chromatin. We found that DNA damage-induced release of histone H2AX, a variant of histone H2A, from damaged chromatin is required for the DNA damage-induced focus formation of RAD51. The release of H2AX from damaged chromatin is regulated by acetylation and ubiquitination of H2AX by TIP60 and UBC13, respectively, We also found that histone H2AZ, another H2A variant, is released from chromatin after induction of DNA damage.

Research Products

• [Journal Article] Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase. (2010)
  • Author(s): Niida H, Katsuno Y, Sengoku M, Shimada M, Yukawa M, Ikura M, Ikura T, Kohno K, Shima H, Suzuki H, Tashiro S, Nakanishi M
  • Journal Title: Genes Dev 24 Pages: 333-8
  • Peer Reviewed
• [Journal Article] Histone H2AX participates the DNA damage-induced ATM activation through interaction with NBS1. (2009)
  • Author(s): Kobayashi J, Tauchi H, Chen B, Bruma S, Tashiro S, Matsuura S, Tanimoto K, Chen DJ, Komatsu K
  • Journal Title: Biochem Biophys Res Commun 380 Pages: 752-757
  • Peer Reviewed
• [Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway. (2008)
  • Author(s): Ishiai M, Kitao H, Smogorzewska A, Tomida J, Kinomura A, Uchida E, Saberi A, Kinoshita E, Kinoshita-Kikuta E, Koike T, Tashiro S, Elledge SJ, Takata M
  • Journal Title: Nat Struct Mol Biol 15(11) Pages: 1138-1146
  • Peer Reviewed
• [Journal Article] The mobility of Bach2 nuclear foci is regulated by SUMO-1 modification. (2008)
  • Author(s): Kono K, Harano Y, Hoshino H, Kobayashi M, Bazzet-Jones D, Muto A, Igarashi K, Tashiro S
  • Journal Title: Exp Cell Res 314 Pages: 903-913
  • Peer Reviewed
• [Journal Article] DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics. (2007)
  • Author(s): Ikura T*, Tashiro S*, Kakino A, Shima H, Jacob N, Amunugama R, Yoder K, Izumi S, Kuraoka I, Tanaka K, Kimura H, Ikura M, Nishikubo S, Ito T, Muto A, Miyagawa K, Takeda S, Fishel R, Igarashi K, Kamiya K
  • Journal Title: Mol Cell Biol 27 Pages: 7028-7040
  • Peer Reviewed
• [Journal Article] Nuclear positioning of the BACH2 gene in BCR-ABL positive leukemia cells. (2007)
  • Author(s): Ono A, Kono K, Ikebe D, Muto A, Sun J, Kobayashi M, Ueda K, Melo JV, Igarashi K, Tashiro S
  • Journal Title: Genes, Chromosomes & Cancer 46 Pages: 67-74
  • Peer Reviewed
• [Presentation] Roland Kanaar and Satoshi Tashiro: ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot in therapy-related leukemia. (2009)
  • Author(s): Jiying Sun, Yukako Oma, Masahiko Harata, Kazuteru Kono, Hiroki Shima, Aiko Kinomura, Tsuyoshi Ikura, Shuki Mizutani
  • Organizer: Keystone symposia on molecular and cellular biology
  • Place of Presentation: Taos, USA
  • Year and Date: 20090228-20090305
• [Presentation] Mechanism of chromosomal translocation involving the MLL gene. (2009)
  • Author(s): Satoshi Tashiro
  • Organizer: International Symposium DNA Damage Response And Repair Mechanism
  • Place of Presentation: Crete, Greek
  • Year and Date: 2009-04-21
• [Presentation] ATM modulates the loading of recombination proteins onto a chromosomal translocation breakpoint hotspot in therapy-related leukemia. (2009)
  • Author(s): Tashiro S
  • Organizer: he 3rd International Open Laboratory Workshop
  • Place of Presentation: Chiba
  • Year and Date: 2009-02-27
• [Presentation] Dynamics of higher order nuclear architecture upon DNA damage. (2008)
  • Author(s): Satoshi Tashiro
  • Organizer: The 7th Japan-France Workshop on Radiation Biology
  • Place of Presentation: Chiba
  • Year and Date: 20081014-20081015
• [Presentation] DISTRIBUTION OF NON-CHROMATIN NUCLEAR DOMAINS IN INTERCHROMATIN COMPARTMENT. (2008)
  • Author(s): Kazuteru Kono, Thomas Cremer, Satoshi Tashiro
  • Organizer: Cold Spring Harbor Laboratory meeting
  • Place of Presentation: New York, USA
  • Year and Date: 20080918-20080921
• [Presentation] Mechanism of chromosomal translocation involving the MLL gene. (2008)
  • Author(s): Jiying SUN, Satoshi Tashiro
  • Organizer: Ataxia-Telangiectasia Workshop
  • Place of Presentation: 大津
  • Year and Date: 20080422-20080426
• [Presentation] 放射線誘発核内ドメインのダイナミクス (2008)
  • Author(s): 田代聡
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] ゲノム修復の細胞核ダイナミクス (2008)
  • Author(s): 田代聡
  • Organizer: 「特定領域研究」2領域合同公開シンポジウム「クロマチンシグナリングの分子機構」
  • Place of Presentation: 東京
  • Year and Date: 2008-01-08
• [Book] Nuclear Architecture: Topology and Function of Chromatin-and Non-Chromatin Nuclear domains(Nagata K, Takeyasu K, ed.) (2007)
  • Author(s): Tashiro S, Cremer M, Solovei I, Cremer T
  • Total Pages: 197-226
  • Publisher: Springer
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/cellbio/Site/18ECC511-D377-11DA-83B6-000A956E89D4.html