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2009 Fiscal Year Final Research Report

Creation and Kinetics Analysis of Biocatalysis-Electrode Interface for Energy Conversion

Research Project

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Project/Area Number 19310070
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanomaterials/Nanobioscience
Research InstitutionKyoto University

Principal Investigator

KANO Kenji  Kyoto University, 農学研究科, 教授 (10152828)

Project Period (FY) 2007 – 2009
Keywordsバイオ電池 / マルチ銅酸化酵素 / フラボヘモプロテイン / 電流-電圧曲線 / 直接電子移動型触媒 / 酵素還元電極反応 / 糖酸化電極反応 / 炭素素材
Research Abstract

Fundamental and applied researches have been performed for biological fuel cell as a potent energy conversion system in future. By focusing on direct electron transfer (DET) type bioelectrocatalytic reactions with enzymes or microbes as catalysts, mechanism of the electron transfer and stability of enzyme in electric double layer have been discussed in detail. On the other hand, methods for preparation of mesoporous carbon material suitable for enzyme immobilization and DET reactions and of bio-electrodes have been presented. Based on these studies, a novel biocathode to show a steady-state current density as high as 20mA cm^<-2> under passive mode, has successfully been constructed. The performance is the best in the world.

  • Research Products

    (48 results)

All 2010 2009 2008 2007 Other

All Journal Article (24 results) (of which Peer Reviewed: 21 results) Presentation (23 results) Remarks (1 results)

  • [Journal Article] Electron Transfer Pathways in Microbial Oxygen Biocathodes.2010

    • Author(s)
      Stefano Freguia, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Electrochim. Acta 55(3)

      Pages: 813-818

    • Peer Reviewed
  • [Journal Article] Electrochemical Reaction of Fructose Dehydrogenase on Carbon Cryogel Electrodes with Controlled Pore Sizes.2010

    • Author(s)
      Seiya Tsujimura, Akiko Nishina, Yasuyuki Hamano, Kenji Kano, Soshi Shiraishi
    • Journal Title

      Electrochem. Commun. 12(3)

      Pages: 446-449

    • Peer Reviewed
  • [Journal Article] マルチ銅酵素を機軸とするカソード触媒.2009

    • Author(s)
      辻村清也, 加納健司
    • Journal Title

      燃料電池 9(1)

      Pages: 62-70

  • [Journal Article] 酵素を使った燃料電池用電極2009

    • Author(s)
      辻村清也, 加納健司
    • Journal Title

      機能材料 29(9)

      Pages: 44-57

  • [Journal Article] Coulometric D-fructose Biosensor Based on Direct Electron Transfer Using D-Fructose Dehydrogenase.2009

    • Author(s)
      Seiya Tsujimura, Akiko Nishina, Yuji Kamitaka, Kenji Kano
    • Journal Title

      Anal. Chem. 81(22)

      Pages: 9383-9387

    • Peer Reviewed
  • [Journal Article] Lactococcus lactis Catalyses Electricity Generation at Microbial Fuel Cell Anodes via Excretion of a Soluble Quinone.2009

    • Author(s)
      Stefano Freguia, Sinnki Masuda, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Bioelectrochemistry 76(1/2)

      Pages: 14-18

    • Peer Reviewed
  • [Journal Article] Air Diffusion Biocathode with CueO as Electrocatalyst Adsorbed on Carbon Particle Modified Electrodes.2009

    • Author(s)
      Ryota Kontani, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Bioelectrochemistry 76(1/2)

      Pages: 10-13

    • Peer Reviewed
  • [Journal Article] Direct Electrochemistry of CueO and Its Mutants at Residues to and Near Type I Cu for Oxygen-Reducing Biocathode.2009

    • Author(s)
      Yuko Miura, Seiya Tsujimura, Sinji Kurose, Yuji Kamitaka, Kenji Kataoka, Takeshi Sakurai, Kenji Kano
    • Journal Title

      Fuel Cells 9(1)

      Pages: 70-78

    • Peer Reviewed
  • [Journal Article] Direct Electrochemistry of Histamine Dehydrogenase from Nocardioides simplex2009

    • Author(s)
      Maiko Tsutsumi, Seiya Tsujimura, Osamu Shirai, Kenji Kano
    • Journal Title

      J. Electroanal. Chem. 625(1)

      Pages: 144-148

    • Peer Reviewed
  • [Journal Article] バイオ電池のための酵素触媒機能電極.2008

    • Author(s)
      辻村清也, 加納健司
    • Journal Title

      Electrochemistry 76(12)

      Pages: 900-909

  • [Journal Article] Coulometric Bioelectrocatalytic Reactions Based on NAD-dependent Dehydrogenases in Tricarboxylic Acid Cycle.2008

    • Author(s)
      Jun Fukuda, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Electrochimica Acta 54(2)

      Pages: 328-333

    • Peer Reviewed
  • [Journal Article] Electrochemical Regulation of the End-product Profile in Propionibacterium freudenreichii ET-3 with an Endogenous Mediator.2008

    • Author(s)
      Yung-Fu Wang, Masaki Masuda, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Biotechnol. Bioengineer. 101(3)

      Pages: 579-586

    • Peer Reviewed
  • [Journal Article] Pentacyanoferrate and Bilirubin Oxidase-bound Polymer for Oxygen Reduction Bio-cathode.2008

    • Author(s)
      Kenji Ishibashi, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Electrochemistry 76(8)

      Pages: 594-596

    • Peer Reviewed
  • [Journal Article] Direct Electron Transfer Reaction of D-Gluconate 2-Dehydrogenase Adsorbed on Bare and Thiol-modifi1ed Gold Electrodes2008

    • Author(s)
      Seiya Tsujimura, Tomohiko Abo, Kazunobu Matsushita, Yoshitaka Ano, Kenji Kano
    • Journal Title

      Electrochemistry 76(8)

      Pages: 549-551

    • Peer Reviewed
  • [Journal Article] CueO-immobilized Porous Carbon Electrode Exhibiting Improved Performance of Electrochemical Reduction of Dioxygen to Water.2008

    • Author(s)
      Seiya Tsujimura, Yuko Miura, Kenji Kano
    • Journal Title

      Electrochimica Acta 53(18)

      Pages: 5716-5720

    • Peer Reviewed
  • [Journal Article] Thermodynamic Redox Properties Governing Half-reduction Characteristics of Histamine Dehydrogenase from Nocardioides simplex.2008

    • Author(s)
      Maiko Tsutsumi, Nobutaka Fujieda, Seiya Tsujimura, Osamu Shirai, Kenji Kano
    • Journal Title

      Biosci. Biotechnol. Biochem. 72(3)

      Pages: 786-796

    • Peer Reviewed
  • [Journal Article] Mediated Bioelectrocatalytic Reaction Using Monolayered Redox Polymer on a Glassy Carbon Electrode Surface and Effect of the Ionic Strength on the Catalytic Current.2008

    • Author(s)
      Seiya Tsujimura, Akio Ishii, Tomohiko Abo, Kenji Kano
    • Journal Title

      J. Electroanal. Chem. 614(1/2)

      Pages: 67-72

    • Peer Reviewed
  • [Journal Article] Electrochemistry of D-Gluconate 2-Dehydrogenase from Gluconobacter frateurii on Indium Tin Oxide Electrode Surface.2007

    • Author(s)
      Seiya Tsujimura, Tomohiko Abo, Yoshitaka Ano, Kazunobu Matsushita, Kenji Kano
    • Journal Title

      Chem. Lett. 36(9)

      Pages: 1164-1165

    • Peer Reviewed
  • [Journal Article] Diffusion-controlled Oxygen Reduction on Multi-copper Oxidase-adsorbed Carbon Aerogel Electrodes without Mediator.2007

    • Author(s)
      Seiya Tsujimura, Yuji Kamitaka, Kenji Kano
    • Journal Title

      Fuel Cells 7(6)

      Pages: 463-469

    • Peer Reviewed
  • [Journal Article] Self-excreted Mediator from Escherichia coli K-12 for Electron Transfer to Carbon Electrodes.2007

    • Author(s)
      Yung-Fu Wang, Seiya Tsujimura, Sheng-Shung Cheng, Kenji Kano
    • Journal Title

      Appl. Microbiol. Biotechnol. 76(6)

      Pages: 1439-1446

    • Peer Reviewed
  • [Journal Article] Fructose/Dioxygen Biofuel Cell Based on Direct Electron Transfer-type Bioelectrocatalysis.2007

    • Author(s)
      Yuji Kamitaka, Seiya Tsujimura, Norihiko Setoyama, Tsutomu Kajino, Kenji Kano
    • Journal Title

      Phys. Chem. Chem. Phys. 9(15)

      Pages: 1793-1801

    • Peer Reviewed
  • [Journal Article] Effects of Oxygen on Shewanella decolorationis NTOU1 Electron Transfer to Carbon Felt Electrodes.

    • Author(s)
      Shiue-Lin Li, Stefano Freguia, Shiu-Mei Liu, Sheng-Shung Cheng, Seiya Tsujimura, Osamu Shirai, Kenji Kano
    • Journal Title

      Biosens. Bioelectron. (in press)

    • Peer Reviewed
  • [Journal Article] Flavins Contained in Yeast Extract are Exploited for Anodic Electron Transfer by Lactococcus lactis.

    • Author(s)
      Masaki Masuda, Stefano Freguia, Yung-Fu Wang, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Bioelectrochemistry (in press)

    • Peer Reviewed
  • [Journal Article] Amperometric Detection of Acetate Based on Mediated Bioelectrocatalysis using Escherichia coli Cells Cultivated with Acetate.

    • Author(s)
      Yung-Fu Wang, Seiya Tsujimura, Kenji Kano
    • Journal Title

      Electrochemistry 76(8)

      Pages: 631-633

    • Peer Reviewed
  • [Presentation] Improved Performance of Oxygen-reducing Biocathode in Biological Fuel Cells.2010

    • Author(s)
      Kano, Kenji, Tsujimura, Seiya
    • Organizer
      217th ECS Meeting
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      20100425-20100430
  • [Presentation] Kenji Kano, Seiya Tsujimura2010

    • Author(s)
      Kenji Kano, Seiya Tsujimura
    • Organizer
      217th ECS Meeting
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      20100425-20100430
  • [Presentation] 生体触媒電極反応とバイオ電池2010

    • Author(s)
      加納健司
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      東大阪市
    • Year and Date
      2010-03-26
  • [Presentation] 酵素機能電極の何を理解して, 何に役立てるのか?2010

    • Author(s)
      加納健司
    • Organizer
      第12回基礎電気化学フォーラム
    • Place of Presentation
      京都
    • Year and Date
      2010-02-13
  • [Presentation] Surface Properties Governing Direct Electron Transfer Kinetics of a Multi-copper Oxidase CueO.2009

    • Author(s)
      Kenji Kano, Seiya Tsujimura
    • Organizer
      216th ECS Meeting
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      20091004-20091009
  • [Presentation] Redox Enzyme-based Bioelectrocatalytic Reactions and Its Application to Biosensors and Biofuel Cells.2009

    • Author(s)
      Kano, Kenji
    • Organizer
      George Wilson's 70th Birthday Symposium
    • Place of Presentation
      Apollo Auditorium, Nichols Hall, The University of Kansas, KS, USA
    • Year and Date
      20090911-20090912
  • [Presentation] Fundamental and Perspective of Bioelectrochemistry.2009

    • Author(s)
      Kenji Kano
    • Organizer
      Osaka Univ. Global COE Program: Dynamics of Biological Systems, Frontier Biosciences GCOE Young Researchers Retreat 2009
    • Place of Presentation
      Rokko Sky Villa, Kobe.
    • Year and Date
      20090824-20090826
  • [Presentation] Direct Electron Transfer of Redox Enzymes and its Application to Biofuel Cells.2009

    • Author(s)
      Kenji Kano
    • Organizer
      The 42nd Heyrovsky Discussion
    • Place of Presentation
      Trest Castle, Czech Republic
    • Year and Date
      20090614-20090618
  • [Presentation] バイオ電池の現状と展望2009

    • Author(s)
      加納健司
    • Organizer
      化学電池材料研究会, 第25回講演会
    • Place of Presentation
      東京
    • Year and Date
      2009-12-16
  • [Presentation] 酵素と電極の接合-シグナル変換からエネルギー変換-2009

    • Author(s)
      加納健司
    • Organizer
      東海コンファレンス, 2009
    • Place of Presentation
      松本
    • Year and Date
      2009-11-06
  • [Presentation] 有機物から電気を取り出すバイオ電池2009

    • Author(s)
      加納健司
    • Organizer
      食品衛生懇話会
    • Place of Presentation
      大阪
    • Year and Date
      2009-09-08
  • [Presentation] 加納健司2009

    • Author(s)
      酵素・微生物バイオ電池の現状
    • Organizer
      (財)バイオインダストリ協会
    • Place of Presentation
      東京
    • Year and Date
      2009-08-07
  • [Presentation] バイオ電池の現状と展望2009

    • Author(s)
      加納健司
    • Organizer
      平成21年度触媒学会燃料電池関連触媒研究会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-17
  • [Presentation] Kinetic Characterization of Multi-copper Oxidase and the Synthesis of Pentacyanoferrate-bound Polymer for Oxygen Reduction Bio-cathode.2008

    • Author(s)
      Kenji Ishibashi, Seiya Tsujimura, Kenji Kano
    • Organizer
      The 214th ECS Meeting (2008 Fall Meeting of The Electrochemical Society of Japan)
    • Place of Presentation
      Honolulu, HI, U.S.A.
    • Year and Date
      20081012-20081017
  • [Presentation] バイオ燃料電池最近の動向と応用展開.2008

    • Author(s)
      加納健司
    • Organizer
      第60回日本生物工学会大会
    • Place of Presentation
      仙台
    • Year and Date
      20080827-20080829
  • [Presentation] Direct Electron Transfer-type Fructose/dioxygen Biofuel Cell.2008

    • Author(s)
      Kenji Kano, Seiya Tsujimura, Yuji Kamitaka
    • Organizer
      213th ECS Meeting
    • Place of Presentation
      Phoenix, Arizona, U.S.A.
    • Year and Date
      20080518-20080522
  • [Presentation] Direct Electron Transfer-type Biofuel Cells.2008

    • Author(s)
      Kenji Kano
    • Organizer
      The 6th Asian Conference on Electrochemistry
    • Place of Presentation
      Chientan Overseas Youth Activity Center, Taipei, Taiwan
    • Year and Date
      20080511-20080514
  • [Presentation] 酢酸菌由来の酵素の生物電気化学的利用.「産業を創出する応用微生物学-酢酸菌バイオテクノロジーの新展開-」2008

    • Author(s)
      加納健司
    • Organizer
      日本農芸化学会2008年度大会
    • Place of Presentation
      名古屋
    • Year and Date
      20080326-20080329
  • [Presentation] 情報変換・エネルギー変換に向けた生物電気化学.2008

    • Author(s)
      加納健司
    • Organizer
      第95回分析技術研究会
    • Place of Presentation
      大阪
    • Year and Date
      2008-06-04
  • [Presentation] 太陽のめぐみをバイオの力で電気に変える.2008

    • Author(s)
      加納健司
    • Organizer
      食品科学工学会関西支部主催市民フォーラム
    • Place of Presentation
      京都
    • Year and Date
      2008-05-09
  • [Presentation] 生体エネルギー変換に学ぶバイオ電池への夢2007

    • Author(s)
      加納健司
    • Organizer
      日本生体エネルギー研究会第33回討論会
    • Place of Presentation
      山口
    • Year and Date
      20071115-20071117
  • [Presentation] 電極触媒としての酸化還元酵素-バイオ電池への夢2007

    • Author(s)
      加納健司
    • Organizer
      第57回錯体化学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      20070925-20070927
  • [Presentation] 次世代型バイオ電池への夢2007

    • Author(s)
      加納健司
    • Organizer
      日本農芸化学会関西支部
    • Place of Presentation
      大阪
    • Year and Date
      2007-07-07
  • [Remarks]

    • URL

      http://www.bapc.kais.kyoto-u.ac.jp/

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Published: 2011-06-18   Modified: 2016-04-21  

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