2009 Fiscal Year Final Research Report
Nanostructure Analysis of Biospecimens with Coherent X-ray Diffraction Microscopy
| Project/Area Number |
19310084
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Nanomaterials/Nanobioscience
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
NISHINO Yoshinori The Institute of Physical and Chemical Research, 石川X線干渉光学研究室, 専任研究員 (40392063)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MAESHIMA Kazuhiro 国立遺伝学研究所, 構造遺伝学研究センター, 教授 (00392118)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Yukio 大阪大学, 大学院・工学研究科・付属フロンティア研究センター, 特任講師 (00415217)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | コヒーレントX線 / 染色体 / X線回折顕微法 / ナノ構造解析 / 位相コントラストイメージング / オルガネラ / 高次構造 / 位相回復 |
|---|
| Research Abstract |
We performed research in bioimaging with x-ray diffraction microscopy on various aspects concerning microscope hardware development, sample preparation, data acquisition, data analysis, etc. We achieved results including first three-dimensional observation of a cellular organelle using hard x-rays leading research in the field. Our study also provided structural information of biological interest. For example, in the chromosome image which we successfully visualized, axial structure was observed first without staining or other methods to artificially enhance image contrast.
|
