Application of the Activated Reaction by Hybridization to Target Genes for the Regulation of Gene Expression in Cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 19350079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Tohoku University
• Principal Investigator: NAGATSUGI Fumi Tohoku University, 多元物質科学研究所, 教授 (90208025)
• Co-Investigator(Kenkyū-buntansha): IMOTO Shuhei 東北大学, 多元物質科学研究所, 助教 (20447189)
• Project Period (FY): 2007 – 2008
• Keywords: 核酸・蛋白質・糖化学 / クロスリンク反応

Research Abstract

本研究では遺伝子を標的とした選択的な化学反応により、細胞内における遺伝子機能を制御する方法、すなわち遺伝子を標的とするIn Cell Chemistryを提案し、その実現にむけた新しい分子の設計・合成、さらには細胞内における機能評価を行い、高機能を有する新規人工遺伝子制御分子の開発を目的とした。その方法論として我々が独自に開発したハイブリダイゼーション活性化反応を細胞内の遺伝子制御方法として展開することを目指し検討を行なった。その結果、非常に有効な方法論を開発できた

Research Products

• [Journal Article] DNA Templated Click Chemistry for Creation of Novel DNA Binding Molecules (2008)
  • Author(s): Imoto S., Hirohama T., Nagatsugi F.
  • Journal Title: Bioorg. Chem. Lett. 18 Pages: 5660-5663
  • Peer Reviewed
• [Journal Article] Synthesis and Evaluation of the Luciferase-Oligodeoxynucleotide for the Sequences-Selective Detection of Nucleic Acids (2008)
  • Author(s): Nagatsugi F., Nakahara R., Inoue K., Sasaki S.
  • Journal Title: Arch. Pharm. Chem. Life Sci. 341 Pages: 562-567
  • Peer Reviewed
• [Journal Article] Synthesis of the Peptide Nucleic Acid(PNA)Incorporating 2-Amino-6-vinylpurine Derivative and Evaluation of the Reactivity (2008)
  • Author(s): Imoto S., Shimotazawa A., Goto M., Hirohama T., Nagatsugi F.
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 391-392
• [Journal Article] Synthesis of the new nucleoside analogue connecting 2-amino-6-vinylpurine to the 2'-deoxyribose skeleton via the methylene linker (2008)
  • Author(s): Kurose Y., Taniguchi Y., Nagatsugi F., Sasaki S.
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 391-392
• [Journal Article] New Methodology to Search for DNA Binding Ligands Based on Duplex DNA-Templated Click Chemistry (2008)
  • Author(s): Imoto S., Hirohama, T., Nagatsugi F.
  • Journal Title: Nucleic Acids Symp. Ser. 52 Pages: 381-382
• [Journal Article] Efficient Synthesis of 6-Substituted Purine Derivatives Using Pd-Catalyzed Cross-Coupling Reactions with 2'-Deoxyguanosine O^6-Tosylate (2007)
  • Author(s): Nagatsugi F.; Ogata, Y ; Imoto, S.; Sasaki, S.
  • Journal Title: Heterocycles 73 Pages: 493-501
  • Peer Reviewed
• [Journal Article] Enzymatic Incorporation of 2-amino-6-Vinylpurine Derivative (2007)
  • Author(s): Imoto, S.; Ali, M. M.; Li, Y.; Sasaki, S.; Nagatsugi, F.
  • Journal Title: Nucleic Acids Symp. Ser. 51 Pages: 357-358
• [Journal Article] Development of the Novel Drug Releasing System Triggered by Hybridization with Target Sequence (2007)
  • Author(s): Nagatsugi, F.; Nakayama, S.; Sasaki, S.
  • Journal Title: Nucleosides, Nucleotides Nucleic Acids 26 Pages: 799-803
  • Peer Reviewed
• [Presentation] 新しいバイオ技術開発に向けた機能分子の創製, 未来を切り拓く女性化学者たち (2008)
  • Author(s): 永次史
  • Organizer: 女性科学者が語る研究最前線
  • Place of Presentation: 北九州(invited)
  • Year and Date: 2008-12-17
• [Presentation] 遺伝子発現の選択的制御を目指した機能性分子の合成 (2008)
  • Author(s): 永次史
  • Organizer: 第2回機能性分子ミニシンポジウム
  • Place of Presentation: 筑波(invited)
  • Year and Date: 2008-09-11
• [Presentation] Synthesis of the Peptide Nucleic Acid(PNA)Incorporating 2-Amino-6-vinylpurine Derivative and Evaluation of the Reactivity (2008)
  • Author(s): S. Imoto, A. Shimotazawa, M. Goto, T. Hirohama, F. Nagatsugi
  • Organizer: Joint Symposium of 18th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids(IRTXVIII)and 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto
  • Year and Date: 2008-09-10
• [Presentation] Development of New DNA Binding Ligand Based on duplex DNA Templated Click Chemistry, Joint Symposium of 18th International Roundtable on Nucleosides (2008)
  • Author(s): T. Hirohama, S. Imoto, F. Nagatsugi
  • Organizer: Nucleotides and Nucleic Acids(IRTXVIII)and 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto
  • Year and Date: 2008-09-10
• [Presentation] Synthesis of the new nucleoside analogue connecting 2-amino-6-vinylpurine to the 2'-deoxyribose skeleton via the methylene linker, Joint Symposium of 18th International Roundtable on Nucleosides (2008)
  • Author(s): Y. Kurose., Y. Taniguchi, F. Nagatsugi, S. Sasaki
  • Organizer: Nucleotides and Nucleic Acids(IRTXVIII)and 35th International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kyoto
  • Year and Date: 2008-09-10
• [Presentation] Development of the New Strategies by Artificial Molecules for Regulation of Gene Expression (2008)
  • Author(s): F. Nagatsugi
  • Organizer: G-COE Summer School
  • Place of Presentation: Sendai, Japan(invite)
  • Year and Date: 2008-08-17
• [Presentation] 遺伝子発現の選択的制御を目指した機能性分子の合成 (2008)
  • Author(s): 永次史
  • Organizer: 第20回, 生体機能関連化学若手の会
  • Place of Presentation: 白石(invited)
  • Year and Date: 2008-08-10
• [Presentation] Synthesis and Evaluation of the Peptide Nucleic Acids(PNAs)Incorporating Reactive Artificial Molecules, XIV Symposium on Chemistry of Nucleic Acid Components (2008)
  • Author(s): S. Imoto, M. Goto, T. Hirohama, F. Nagatsugi
  • Organizer: Czesky Krumlov
  • Place of Presentation: Czech Republic
  • Year and Date: 2008-06-10
• [Presentation] Enzymatic Incorporation of 2-amino-6-Vinylpurine Derivative (2007)
  • Author(s): S.Imoto, M. M. Ali, Y. Li, S. Sasaki, F. Nagatsugi
  • Organizer: 5^<th> International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2007-11-21
• [Presentation] Application of the Inducible Reactive Oligonucleotides for Novel Drug Releasing System Triggered by Hybridization to Target Genes (2007)
  • Author(s): F. Nagatsugi
  • Organizer: 4^<th> Japanese-Sino Symposium on Organic Chemistry for Young Scientists
  • Place of Presentation: Narita, Japan(invited)
  • Year and Date: 2007-09-26
• [Presentation] Development of the Methodology for Searching New Binding Molecules to highly-Ordered DNA Structure (2007)
  • Author(s): F. Nagatsugi, S. Imoto
  • Organizer: The 2^<nd> International Workshop on Approaches to Single-cell Analysis
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2007-09-06
• [Presentation] Application of the Inducible Reactive Oligonucleotides for Novel Drug Releasing System Triggered by Hybridization (2007)
  • Author(s): F. Nagatsugi, S. Nakayma, S. Mori, S. Sasaki
  • Organizer: Gordon Conferences
  • Place of Presentation: New Port, USA
  • Year and Date: 2007-06-28
• [Presentation] 細胞内における化学的遺伝子発現制御を目指したアプローチ (2007)
  • Author(s): 永次史
  • Organizer: 東北大学学際科学国際高等研究センターフォーラム「生命科学における学際研究の最前線
  • Place of Presentation: 仙台(invited)
  • Year and Date: 2007-01-26
• [Presentation] 機能性人工核酸を用いた遺伝子発現制御による新しい創薬手法の開発 (2007)
  • Author(s): 永次史
  • Organizer: 第8回創薬ビジョンシンポジウム
  • Place of Presentation: 京都(invited)
  • Year and Date: 2007-01-25
• [Remarks] 永次史、機能性人工核酸を用いた遺伝子発現制御による新しい創薬手法の開発Pharma VISION NEWS, No.9, 24-28(2007)
• [Remarks] 永次史、遺伝子発現の化学的な制御を目指した人工分子の開発、ペプチド研究会レター、No.66, 4-6(2007)