2009 Fiscal Year Final Research Report
Structural basis of functional changes of RNA modification enzymes through evolution of life
| Project/Area Number |
19350087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Ehime University |
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Principal Investigator |
HORI Hiroyuki Ehime University, 大学院・理工学研究科, 教授 (20256960)
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| Co-Investigator(Kenkyū-buntansha) |
NUREKI Osamu 東京大学, 医科学研究所, 教授 (10272460)
YOKOBORI Shin-ichi 東京薬科大学, 生命科学部, 講師 (40291702)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 酵素化学 |
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| Research Abstract |
Living organisms can be classified to three domains, eukaryotes, archaea and eubacteria. The purpose of this study is clarification of structural and functional differences of RNA modification enzymes according to the evolution of life. Our new findings are as follows : discovery of new multisite-specificity RNA methyltransferase, structural study and hypothetical reaction mechanism of folate-dependent RNA methyltransferase, multistep recognition of substrate tRNA by protein-knot RNA methyltransferase, and discovery of RNA modification network.
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[Journal Article] Aquifex aeolicus tRNA (N2, N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA.2009
Author(s)
T. Awai, S. Kimura, C. Tomikawa, A. Ochi, Ihsanawati, Y. Bessho, S. Yokoyama, S. Ohno, K. Nishikawa, T. Yokogawa, T. Suzuki, H. Hori
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Journal Title
J. Biol. Chem. 284
Pages: 20467-20478
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[Journal Article] Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing2007
Author(s)
M. Abe, S. Ohno, T. Yokogawa, T. Nakanishi, F. Arisaka, T. Hosoya, T. Hiramatsu, M. Suzuki, T. Ogasawara, T. Sawasaki, K. Nishikawa, M. Kitamura, H. Hori, Y. Endo
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Journal Title
Proteins: Structure, Function and Bioinformatics 67
Pages: 643-652
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[Presentation] Transfer RNA Methyltransferases from a Hyperthermophilic Eubacterium, Aquifex aeolicus2010
Author(s)
H. Hori, T. Awai, T. Toyooka, C. Tomikawa, H. Okamoto, H. Takeda, K. Watanabe, A. Ochi, A. Hirata, S. Kimura, Y. Ikeuchi, T. Yokogawa, T. Suzuki
Organizer
23rd tRNA workshop Portugal 2010
Place of Presentation
Aveiro, Portugal
Year and Date
2010-01-28
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[Presentation] Multi-site substrate recognition mechanism of Aquifex aeolicus Trm12010
Author(s)
T. Awai, Ihsanawati, S Kimura, C. Tomikawa, A. Ochi, T. Yokogawa, T. Suzuki, Y. Bessho, S. Yokoyama, H. Hori
Organizer
23rd tRNA workshop Portugal 2010
Place of Presentation
Aveiro, Portugal
Year and Date
2010-01-28
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