2009 Fiscal Year Final Research Report
Nonlinear Raman microscopy to detect membrane protein
| Project/Area Number |
19360033
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied optics/Quantum optical engineering
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
HASHIMOTO Mamoru Osaka University, 大学院・基礎工学研究科, 准教授 (70237949)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Shuichiro 大阪大学, 大学院・基礎工学研究科, 助教 (40362644)
FUJITA Katsumasa 大阪大学, 大学院・工学研究科, 准教授 (80362664)
YOSHIKI Keisuke 兵庫県立大学, 大学院・工学研究科, 助教 (60432548)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | ラマン散乱顕微鏡 / 非線形光学効果 / 振動分光学 |
|---|
| Research Abstract |
We developed a multi-focus excitation nonlinear Raman microscope using a microlens array scanner for real-time molecular imaging. Parallel exposure of a specimen with light from two highly controlled ps mode-locked lasers (jitter of 30fs, point-by-point wavelength scan within 300ms) and parallel detection with an image sensor enabled real-time imaging. We demonstrated real-time Raman imaging of living cells (frame rate of 10fps). We also combined the developed system with the laser ablation to destruct the cell membrane, and demonstrated to observe the membrane repairing process. We added the polarization pattern control system to a nonlinear Raman microscopy system to detect membrane proteins by control the direction of electro-magnetic filed on the focus spot. The possibility of the selective detection of ordered molecules was confirmed with the imaging of liquid crystal.
|
