2009 Fiscal Year Final Research Report
Molecular mechanism for the accurate recognition of homologous sequences
| Project/Area Number |
19370004
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SHIBATA Takehiko The Institute of Physical and Chemical Research, 柴田遺伝制御科学研究室, 上席研究員 (70087550)
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| Co-Investigator(Kenkyū-buntansha) |
MIKAWA Tsutomu 独立行政法人理化学研究所, 城生体金属科学研究室, 専任研究員 (20321820)
INOUE Jin 独立行政法人理化学研究所, 柴田遺伝制御科学研究室, 協力研究員 (10469893)
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| Research Collaborator |
LING Feng 独立行政法人理化学研究所, 吉田化学遺伝学研究室, 専任研究員
KURUMIZAKA Hitoshi 早稲田大学, 理工学術院, 教授
KAGAWA Wataru 早稲田大学, 理工学術院, 講師
ARAI Naoto 日本大学, 生物資源科学部, 講師
MASUDA Tokiha 横浜市立大学, 大学院・生体超分子科学専攻・博士後期課程, 学生
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| Project Period (FY) |
2007 – 2009
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| Keywords | ゲノム構築・機能・再編・発現・維持 / 相同DNA組換え / RecA / Rad51型相同対合蛋白質 / ATP非依存型相同DNA対合蛋白質 / 組換えメディエーター / ヘテロ二重鎖形成 / 並行三重鎖DNA / DNAトポロジー |
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| Research Abstract |
Homologous DNA recombination plays roles in the maintenance of genome integrity and in genetic diversification. While RecA/Rad51 homologous pairing proteins pairs DNA molecules with an exact sequence matching by an ATP-hydrolysis-dependent reaction, ATP-independent pairing proteins is unlikely to have such a function. To understand the molecular bases of the recombination functions, we compared RecA/Rad51 and ATP-independent homologous pairing proteins in their reactions. NMR analyses indicate that basic mechanisms of homologous pairing are common independent of the proteins. On the other hand, biochemical and topological studies suggest that an ATP-independent protein generates triplexes, instead of D-loops which RecA/Rad51 produces. These results suggest roles of the two groups of the proteins in homologous recombination.
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Research Products
(32 results)
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[Journal Article] Identification of a second DNA binding site in the human Rad52 protein2008
Author(s)
Kagawa, W., Kagawa, A., Saito, K., Ikawa, S., Shibata, T., Kurumizaka, H., Yokoyama, S.
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Journal Title
J. Biol. Chem. 283
Pages: 24264-24273
Peer Reviewed
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[Journal Article] Struc-tural and functional analyses of the DMC1-M200V polymorphism found in the human population2008
Author(s)
Hikiba, J., Hirota, K., Kagawa, W., Ikawa, S., Kinebuchi, T., Sakane, I., Takizawa, Y., Yokoyama, S., Man-don-Pepin, B., Nicolas, A., Shibata, T., Ohta, K., Kurumizaka, H.
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Journal Title
Nucleic Acids Res. 36
Pages: 4181-4190
Peer Reviewed
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[Journal Article] Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins2008
Author(s)
Sakane, I., Kamataki, C., Takizawa, Y., Nakashima, M., Toki, S., Ichikawa, H., Ikawa, S., Shibata, T., Kurumizaka, H.
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Journal Title
Nucleic Acids Res. 36
Pages: 4266-4276
Peer Reviewed
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