Analysis of oscillatory mechanism of the circadian clock and its function in hemimetabolous insects

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19370029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: Okayama University
• Principal Investigator: TOMIOKA Kenji Okayama University, 大学院・自然科学研究科, 教授 (30136163)
• Project Period (FY): 2007 – 2010
• Keywords: 概日リズム / 光周性 / 体内時計 / 時計遺伝子 / RNAi

Research Abstract

不完全変態の直翅目昆虫コオロギと無翅目昆虫シミの概日時計の分子機構を明らかにすることにより、昆虫時計発振の分子機構の一般性と多様性を検討するとともに、その行動リズムおよび光周性への関与を明らかにすることを目的とする。具体的には、(1)各種光条件下での時計遺伝子mRNAの発現リズムの解析、(2)時計蛋白質の発現リズムと細胞内挙動の解析、(3)RNA干渉を利用した振動機構および光周測時機構における時計遺伝子機能の解析の3点に絞って解析を進め、直翅目および無翅目昆虫での概日時計発振の分子機構の解明とその行動および光周測時機構における役割の解明を目指す。

Research Products

• [Journal Article] Circadian oscillations outside the optic lobe in the cricket Gryllus bimaculatus (2010)
  • Author(s): Uryu O, Tomioka K
  • Journal Title: Journal of Insect Physiology (in press)
• [Journal Article] The clock gene period plays an essential role in photoperiodic control of nymphal development in the cricket Modicogryllus siamensis (2009)
  • Author(s): Sakamoto T, Uryu O, Tomioka K
  • Journal Title: Journal of Biological Rhythms 24 Pages: 379-390
• [Journal Article] A comparative view of insect circadian clock systems (2009)
  • Author(s): Tomioka K, Matsumoto A
  • Journal Title: Cellular and Molecular Life Sciences 67 Pages: 1397-1406
• [Journal Article] RNA interference is a powerful tool for chronobiological study in the cricket (2009)
  • Author(s): Tomioka K, Sakamoto T, Moriyama Y
  • Journal Title: Sleep and Biological Rhythms 7 Pages: 144-151
• [Journal Article] RNA interference of the clock gene period disrupts circadian rhythms in the cricket Gryllus bimaculatus (2008)
  • Author(s): Moriyama Y, Sakamoto T, Karpova SG, Matsumoto A, Noji S, Tomioka K
  • Journal Title: Journal of Biological Rhythms 23 Pages: 308-318
• [Presentation] Dissection of the cricket circadian system with molecular tools (2009)
  • Author(s): Tomioka K
  • Organizer: International Symposium on Biological Rhythm, Joint Symposium on Photonic Bioimaging
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20090801-20090804
• [Presentation] Functional analysis of the clock gene period and Clock with RNA intereference in the cricket Gryllus bimaculatus (2009)
  • Author(s): Moriyama Y, Sakamoto T, Tomioka K
  • Organizer: International Symposium on Biological Rhythm, Joint Symposium on Photonic Bioimaging
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 20090801-20090804
• [Presentation] period gene plays a role in circadian rhythm and photoperiodism in crickets (2008)
  • Author(s): Tomioka K, Moriyama Y, Sakamoto T
  • Organizer: XXIII International Congress of Entomology
  • Place of Presentation: Durban, South Africa
  • Year and Date: 20080706-20080712
• [Presentation] 時計遺伝子RNA干渉によるフタホシコオロギ period 遺伝子の機能解析 (2008)
  • Author(s): 守山禎之・富岡憲治
  • Organizer: 第79回日本動物学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-09-06
• [Presentation] 時計遺伝子RNA干渉によるフタホシコオロギ成虫の概日時計の停止 (2007)
  • Author(s): 守山禎之・富岡憲治
  • Organizer: 第78回日本動物学会大会
  • Place of Presentation: 弘前市
  • Year and Date: 2007-09-23
• [Book] 昆虫の体内時計, 動物の多様な生き方1見える光, 見えない光, 動物と光のかかわり, (寺北明久, 蟻川謙太郎, 編) (2009)
  • Author(s): 富岡憲治
  • Total Pages: 174-192
  • Publisher: 共立出版
• [Book] 昆虫の時計遺伝子と光周性, 光周性の分子生物学, (海老原史樹文, 井澤毅, 編) (2009)
  • Author(s): 富岡憲治
  • Total Pages: 112-119
  • Publisher: シュプリンガー・ジャパン
• [Book] 虫の脳に組み込まれた時計の謎, (山口恒夫, 監修), 昆虫はスーパー脳 (2008)
  • Author(s): 富岡憲治
  • Total Pages: 159-184
  • Publisher: 技術評論社