How does firefly luciferase control the bioluminescence reaction?

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: NAKATSU Toru Kyoto University, 薬学研究科, 准教授 (50293949)
• Co-Investigator(Kenkyū-buntansha): HIRATAKE Jun 京都大学, 化学研究所, 教授 (80199075) ; KATO Taiichirou 兵庫県立大学, 工学研究科, 助教 (60423901) ; KIMURA Yasuhisa 京都大学, 農学研究科, 助教 (10415143)
• Project Period (FY): 2007 – 2009
• Keywords: 構造生物学

Research Abstract

We measure the bioluminescent activities and determine the crystal structures of G326S and Y257A firefly luciferase mutants which emit red light. In the result, it is supposed that the emission color change from yellow-green to red and the decrease of the emission are caused by the energy loss of the excited oxyluciferin. In addition we determine the crystal structure of luciferin regenerating enzyme, which controls the emission reaction in firefly.

Research Products

• [Journal Article] Deleting two C-terminal alpha-helices is effective to crystallize the bacterial ABC transporter Escherichia coli MsbA complexed with AMP-PNP (2010)
  • Author(s): Terakado K, Kodan A, Nakano H, Kimura Y, Ueda K, Nakatsu T, Kato H.
  • Journal Title: Acta Crystallogr D Vol.66 Pages: 319-323
  • Peer Reviewed
• [Journal Article] 高機能化ホタル・ルシフェラーゼ創製に向けて (2010)
  • Author(s): 中津亨
  • Journal Title: 日本結晶学会誌 52(1) Pages: 81-88
• [Journal Article] X-ray crystallographic study of an HIV-1 fusion inhibitor with the gp41 S138A substitution. (2009)
  • Author(s): Watabe T, Terakawa Y, Watanabe K, Ohno H, Nakano H, Nakatsu T, Kato H, Izumi K, Kodama E, Matsuoka M, Kitaura K, Oishi S, Fujii N.
  • Journal Title: J Mol Biol. Vol.392 Pages: 657-665
  • Peer Reviewed
• [Journal Article] Functional role of the linker region in purified human P-glycoprotein. (2009)
  • Author(s): Sato T, Kodan A, Kimura Y, Ueda K, Nakatsu T, Kato H.
  • Journal Title: FEBS J. Vol.276 Pages: 3504-3516
  • Peer Reviewed
• [Journal Article] Structural basis for gibberellin recognition by its receptor GID1 (2008)
  • Author(s): Shimada A, Ueguchi-Tanaka M, Nakatsu T, Nakajima M, Naoe Y, Ohmiya H, Kato H, Matsuoka M.
  • Journal Title: Nature Vol.456 Pages: 520-523
  • Peer Reviewed
• [Journal Article] Mechanism of efficient firefly bioluminescence via adiabatic transition state and seam of sloped conical intersection (2008)
  • Author(s): Chung LW, Hayashi S, Lundberg M, Nakatsu T, Kato H, Morokuma K.
  • Journal Title: J Am Chem Soc. Vol.130 Pages: 12880-12881
  • Peer Reviewed
• [Journal Article] A direct repeat of E-box-like elements is required for cell-autonomous circadian rhythm of clock genes (2008)
  • Author(s): Nakahata Y, Yoshida M, Takano A, Soma H, Yamamoto T, Yasuda A, Nakatsu T, Takumi T.
  • Journal Title: BMC Mol Biol. 1
  • Peer Reviewed
• [Presentation] ホタル・ルシフェラーセによるπ空間制御機構 (2009)
  • Author(s): 寺角香菜子、中津亨
  • Organizer: 日本生物物理学会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2009-10-30
• [Presentation] ホタルルシフェラーゼは基質の不斉をどのように見分けているのか? (2009)
  • Author(s): 加藤太一郎, 宮永佳奈, 番匠亜沙美, 中津亨, 武尾正弘, 根来誠司
  • Organizer: 日本生物工学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-24
• [Presentation] ホタルにおける発光制御メカニズム (2009)
  • Author(s): 中津亨
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2009-03-28
• [Presentation] ルシフェリン再生酵素のX線結晶構造解析 (2009)
  • Author(s): 奥田智彦、五味恵子、梶山直樹、加藤博章、中津亨
  • Organizer: 日本薬学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] ゲンジボタル・ルシフェラーゼの発光反応および発光色制御機構の構造基盤 (2008)
  • Author(s): 中津亨
  • Organizer: 酵素工学研究会
  • Place of Presentation: 京都テルサ
  • Year and Date: 2008-04-25