Molecular basis for recognition of various cell surface ligands by paired immune receptors

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: MAENAKA Katsumi Kyushu University, 生体防御医学研究所, 准教授 (10322752)
• Project Period (FY): 2007 – 2009
• Keywords: ペア型レセプター / 細胞表面受容体 / 蛋白質 / 蛋白質間相互作用 / 表面プラズモン共鳴 / NMR解析 / X線結晶構造解析 / 免疫制御

Research Abstract

Cell surface antigens have various forms depending on states of cells, whose structural characteristics can be recognized by paired immune receptors to control immune responses. Here, in order to clarify the molecular basis for these events, we performed the ligand binding and structural analyses of PILRs and KLRG1. PILRs bind to its ligand, CD99, with sugar- and peptide-dependent manner, furthermore, KLRG1 discriminate the monomer and the dimer configurations of the E-cadherin. These results provided important insights on the immune regulation by paired immune cell receptors.

Research Products

• [Journal Article] Molecular basis for E-cadherin recognition by killer cell lectin-like receptor G1 (KLRG1). (2009)
  • Author(s): Nakamura S, Kuroki K, Ohki I, Sasaki K, Kajikawa M, Maruyama T, Ito M, Kameda Y, Ikura M, Yamamoto K, Matsumoto K, Maenaka K*
  • Journal Title: J Biol Chem. 284 Pages: 27327-35
• [Journal Article] Biophysical characterization of O-glycosylated CD99 recognition by paired Ig-like type 2 receptors (PILR). (2008)
  • Author(s): Tabata S, Kuroki K, Wang J, Kajikawa M, Shiratori I, Kohda D, Arase H, Maenaka K*
  • Journal Title: J Biol Chem. 283 Pages: 8893-901
• [Journal Article] Expression, crystallization and preliminary X-ray diffraction analysis of human paired Ig-like type 2 receptor alpha (PILRalpha). (2008)
  • Author(s): Tabata S, Kuroki K, Maita N, Wang J, Shiratori I, Arase H, Kohda D, Maenaka K*
  • Journal Title: Acta Cryst. F 64 Pages: 44-6
• [Journal Article] Crystal structure of measles virus hemagglutinin provides novel insight into effective vaccines. (2007)
  • Author(s): Hashiguchi T, Kajikawa M, Maita N, Takeda M, Kuroki K, Sasaki K, Kohda D, Yanagi Y*, Maenaka K*
  • Journal Title: Proc. Natl. Acad. Sci. USA 104 Pages: 19535-40
• [Presentation] Molecular basis for E-cadherin recognition by killer cell lectin-like receptor G1 (KLRG1) (2008)
  • Author(s): Nakamura, ら
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 20081201-20081203
• [Presentation] 生体防御に関わる細胞表面受容体の分子認識機構 (2008)
  • Author(s): 前仲勝実
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 20081201-20081203
• [Presentation] Molecular Basis for Recognition of Paired Ig-Like type2 Receptor(PILR) (2007)
  • Author(s): Tabata, et al.
  • Organizer: 第37回日本免疫学会総会
  • Place of Presentation: 東京・品川
  • Year and Date: 20071120-20071122
• [Book] 薬系免疫学 (2007)
  • Author(s): 前仲勝実
  • Total Pages: 238
  • Publisher: 南江堂