2008 Fiscal Year Final Research Report
Studies on gating and permission mechanisms for maturation of secretory cargo proteins
| Project/Area Number |
19370044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
WADA Ikuo Fukushima Medical University, 医学部, 教授 (40182969)
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| Co-Investigator(Kenkyū-buntansha) |
HATSUZAWA Kiyotaka 福島県立医科大学, 医学部, 准教授 研究者 (20256655)
HASHIMOTO Hitoshi 福島県立医科大学, 医学部, 助教 (50372826)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANISHI Hideki 福県立医科大学, 医学部, 助教 (30457783)
HIGASHI Tsunehito 福県立医科大学, 医学部, 助教 (90453018)
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| Project Period (FY) |
2007 – 2008
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| Keywords | 細胞小器官 / ダイナミクス / 一分子トラッキング / 糖鎖 |
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| Research Abstract |
To understand why mammalian cells are capable of producing secreted proteins of high quality in a highly coordinated manner, we have developed a system to examine single molecular dynamics of cargo proteins in the endoplasmic reticulum. Series of analyses have indicated that lumen of the ER are equipped with a machinery that restricts simple diffusion of cargo proteins through a grip of N-linked oligosaccharides using actin cytoskeleton. Since this regulation becomes most prominent at higher temperature, we propose that this constitutes a basic machinery for cargo maturation.
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[Journal Article] Carboxyl-Terminal Disulfide Bond of Acid Sphingomyelinase Is Critical for Its Secretion and Enzymatic Function.2007
Author(s)
Lee C-Yin, Tamura T, Rabah N, Lee D-Y, Ruel I, Hafiane A, Iatan I, Nyholt D, Laporte F, Lazure C, Wada I, Krimbou L, Genest J.
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Journal Title
Biochemistry 46(51)
Pages: 14969-14978
Peer Reviewed
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