Mechanism of the removal of degenerated own cells by Draper, a phagocytosis receptor of Drosophila

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kanazawa University
• Principal Investigator: NAKANISHI Yoshinobu Kanazawa University, 薬学系, 教授 (40172358)
• Co-Investigator(Renkei-kenkyūsha): SHIRATSUCHI Akiko 金沢大学, 薬学系, 准教授 (90303297)
• Project Period (FY): 2007 – 2009
• Keywords: 貪食 / アポトーシス / 自然免疫 / ショウジョウバエ / 細菌感染 / 細胞内情報伝達 / リン酸化

Research Abstract

線虫の貪食受容体CED-1に相当するショウジョウバエのDraperは、血球細胞及びグリア細胞によるアポトーシス細胞の貪食除去に必要とされる。本研究では、Draperが認識するアポトーシス細胞側の目印分子が探索され、研究代表者らがPretaporterと名付けた小胞体タンパク質が見いだされた。Pretaporterはアポトーシス時に小胞体から細胞表層に移動してDraperに認識されるようになると考えられた。また、Pretaporterの結合によりDraperのチロシンリン酸化が促され、それを契機にして細胞内に貪食誘導性の情報が伝達されると示唆された。

Research Products

• [Journal Article] a Drosophila protein serving as a ligand for Draper in the phagocytosis of apoptotic cells (2009)
  • Author(s): Kuraishi, T, Nakagawa, Y., Nagaosa, K., Hashimoto, Y., Ishimoto, T., Moki, T., Fujita, Y., Nakayama, H., Dohmae, N., Shiratsuchi, A., Yamamoto, N., Ueda, K., Yamaguchi, M., Awasaki, T., Nakanishi, Y. Pretaporter
  • Journal Title: EMBO J. 28 Pages: 3868-3878
  • Peer Reviewed
• [Journal Article] A. Identification of lipoteichoic acid as a ligand for Draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): Hashimoto, Y., Tabuchi, Y., Sakurai, K., Kutsuna, M., Kurokawa, K., Awasaki, T., Sekimizu, K., Nakanishi, Y., Shiratsuchi
  • Journal Title: J. Immunol. 183 Pages: 7451-7460
  • Peer Reviewed
• [Journal Article] Y. Identification of calreticulin as a marker for phagocytosis of apoptotic cells in Drosophila (2007)
  • Author(s): Kuraishi, T., Manaka, J., Kono, M., Ishii, H., Yamamoto, N., Koizumi, K., Shiratsuchi, A., Lee, B.L., Higashida, H., Nakanishi
  • Journal Title: Exp. Cell Res. 313 Pages: 500-510
  • Peer Reviewed
• [Presentation] リポタイコ酸とDraperを介したショウジョウバエヘモサイトによる黄色ブドウ球菌の貪食 (2009)
  • Author(s): 橋本優実、田淵幸幾、黒川健児、粟崎健、関水和久、中西義信、白土明子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2009-10-23
• [Presentation] Role of Pretaporter as a ligand for Draper in phagocytosis of apoptotic cells by Drosophila hemocytes (2009)
  • Author(s): Y. Fujita, Y. Nakagawa, T. Kuraishi, Y. Hashimoto, R. Okada, A. Shiratsuchi, Y. Nakanishi
  • Organizer: 17th International Symposium on Molecular Cell Biology of Macrophage
  • Place of Presentation: KKRホテル金沢(石川県)
  • Year and Date: 2009-07-03
• [Presentation] Mechanism and role of Draper-mediated phagocytosis of Staphylococcus aureus by Drosophila hemocytes (2009)
  • Author(s): A. Shiratsuchi
  • Organizer: Gordon Research Conferences: Apoptotic Cell Recognition & Clearance
  • Place of Presentation: Colby-Sawyer College(米国)
  • Year and Date: 2009-07-02
• [Presentation] Gordon Research Conferences (2009)
  • Author(s): T. Kuraishi、Pretaporter, a Draper ligand
  • Organizer: Apoptotic Cell Recognition & Clearance
  • Place of Presentation: Colby-Sawyer College(米国)
  • Year and Date: 2009-06-30
• [Presentation] Pretaporter, a Drosophila endoplasmic reticulum protein serving as a ligand for Draper in phagocytosis of apoptotic cells (2009)
  • Author(s): T. Kuraishi, Y. Nakagawa, T. Ishimoto, T. Moki, Y. Fujita, H. Nakayama, N. Dohmae, A. Shiratsuchi, Y. Hashimoto, N. Yamamoto, K. Ueda, M. Yamaguchi, T. Awasaki, Y. Nakanishi
  • Organizer: Jacques Monod Conferences
  • Place of Presentation: Aussoisホール(フランス)
  • Year and Date: 2009-05-24
• [Presentation] ショウジョウバエCed-6のアポトーシス細胞貪食における役割 (2008)
  • Author(s): 倉石貴透、真中純子、粟崎健、中川祐紀子、荒井國三、岡田亮、白土明子、中西義信
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2008-12-10
• [Presentation] ショウジョウバエ貪食受容体が認識するアポトーシス細胞側リガンドの同定 (2008)
  • Author(s): 中西義信、倉石貴透、石本聖、茂木武、中川祐紀子、中山洋、堂前直、白土明子、橋本優実、山本奈央子、上田晃一、山口政光、粟崎健
  • Organizer: 第17回日本アポトーシス研究会学術集会
  • Place of Presentation: メルパルク京都(京都府)
  • Year and Date: 2008-08-01
• [Presentation] Pretaporter, a Drosophila endoplasmic reticulum protein serving as a ligand for draper in phagocytosis of apoptotic cells (2008)
  • Author(s): T. Kuraishi, T. Ishimoto, T. Moki, Y. Nakagawa, H. Nakayama, A. Shiratsuchi, Y. Hashimoto, N. Yamamoto, K. Ueda, M. Yamaguchi, T. Awasaki, Y. Nakanishi
  • Organizer: The Seventh International Cell Death Society Symposium
  • Place of Presentation: Shanghai National Accounting Institute(中国)
  • Year and Date: 2008-06-06
• [Presentation] Phagocytic removal of apoptotic cells in Drosophila: identification of a receptor and ligand (2008)
  • Author(s): Y. Nakanishi
  • Organizer: Spring International Convention of the Pharmaceutical Society of Korea
  • Place of Presentation: International Convention Center Jeju(韓国)
  • Year and Date: 2008-05-01
• [Presentation] Characterization of CG1837 protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor (2008)
  • Author(s): T. Kuraishi, T. Ishimoto, N. Yamamoto, K. Ueda, H. Nakayama, M. Yamaguchi, T. Awasaki, Y. Hashimoto, T. Moki, A. Shiratsuchi, Y. Nakanishi
  • Organizer: 49th Annual Drosophila Research Conference
  • Place of Presentation: Town and Country Resort & Convention Center(米国)
  • Year and Date: 2008-04-04
• [Presentation] ショウジョウバエ貪食受容体Draperが認識するアポトーシス細胞表層分子の同定 (2007)
  • Author(s): 倉石貴透, 山本奈央子, 上田晃一, 中山洋, 橋本優実, 白土明子, 中西義信
  • Organizer: 第80回日本生化学会大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2007-12-12
• [Presentation] ショウジョウバエ感染症モデルを用いた細菌病原性と宿主免疫応答の解析 (2007)
  • Author(s): 中西義信
  • Organizer: 日本薬学会関東支部第32回学術講演会
  • Place of Presentation: 日本薬学会長井記念会館長井記念ホール(東京都)
  • Year and Date: 2007-10-20
• [Presentation] Hashimoto, H. Nakayama, A. Shiratsuchi, Y. Nakanishi、Isolation of an endoplasimic reticulum protein as a candidate ligand for Draper, a Drosophila phagocytosis receptor (2007)
  • Author(s): T. Kuraishi, N. Yamamoto, K. Ueda, Y. Hashimoto, H. Nakayama, A. Shiratsuchi, Y. Nakanishi
  • Organizer: 第8回日本ショウジョウバエ研究集会
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2007-07-02
• [Presentation] Isolation of putative ligand for Draper, Drosophila homologue of C. elegans CED-1, responsible for apoptotic cell clearance and axonal pruning (2007)
  • Author(s): Y. Nakanishi
  • Organizer: Gordon Conference Apoptotic Cell Recognition and Clearance
  • Place of Presentation: Bates College(米国)
  • Year and Date: 2007-06-19
• [Book] 実験医学増刊号、細胞死による多様な生体制御、第3章の2. アポトーシス依存的な細胞貪食による生体恒常性の維持 (2010)
  • Author(s): 白土明子、中西義信
  • Total Pages: 1055-1061
  • Publisher: 羊土社
• [Book] arget Pattern Recognition in Innate Immunity, U. Kishore (編), 第9章(Pattern recognition in phagocytic clearance of altered self) (2009)
  • Author(s): Nakanishi, Y., Henson, P. M.
  • Total Pages: 129-138
  • Publisher: Landes Bioscience
• [Remarks]
  • URL: http://www.p.kanazawa-u.ac.jp/~seibutu/