A study on lipid dynamics during pathogen infection

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370058
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MAEHAMA Tomohiko National Institute of Infectious Diseases, 細胞化学部, 室長 (40322755)
• Project Period (FY): 2007 – 2009
• Keywords: ウイルス / 感染症 / 脂質 / シグナル伝達

Research Abstract

Cellular lipids function as building blocks of biomembranes and signaling molecules thereby being fundamental components for all cells. In this study, we analyzed the function of cellular lipids for infection cycle of hepatitis C virus and infectious prion. We found that certain types of phosphoinositide were required for replication process of human hepatitis C virus. In addition, we found that the inhibition of sphingolipid biosynthesis substantially suppressed cell-to-cell transmission of infectious prion using newly developed infectious prion transmission assay system.

Research Products

• [Journal Article] An improved method for cell-to-cell transmission of infectious prion. (2010)
  • Author(s): Tanaka, M., Hara, H., Nishina, H., Hanada, K., Hagiwara, K., Maehama, T.
  • Journal Title: Biochem. Biophys. Res. Commun. (in press)
  • Peer Reviewed
• [Journal Article] Role of Rap1B and tumor suppressor PTEN in the negative regulation of lysophosphatidic acid-induced migration by isoproterenol in glioma cells. (2009)
  • Author(s): Malchinkhuu, E., Sato, K., Maehama, T., Ishiuchi, S., Yoshimoto, Y., Mogi, C., Kimura, T., Kurose, H., Tomura, H., Okajima, F.
  • Journal Title: Mol. Biol. Cell 20 Pages: 5156-5165
  • Peer Reviewed
• [Journal Article] アミノ酸感知シグナル-mTOR活性化の分子機構- (2009)
  • Author(s): 前濱朝彦
  • Journal Title: アミノ酸研究 3 Pages: 5-14
  • Peer Reviewed
• [Journal Article] アミノ酸感知シグナル (2009)
  • Author(s): 前濱朝彦
  • Journal Title: 細胞工学 28 Pages: 760-764
  • Peer Reviewed
• [Journal Article] RalA functions as an indispensable signal mediator for the nutrient-sensing system. (2008)
  • Author(s): Maehama, T., Tanaka, M., Nishina, H., Murakami, M., Kanaho, Y., Hanada, K.
  • Journal Title: J. Biol. Chem. 283 Pages: 35053-35059
  • Peer Reviewed
• [Journal Article] あらたな癌抑制遺伝子PICT-1/GLTSCR2 (2008)
  • Author(s): 前濱朝彦、岡原史明、金保安則
  • Journal Title: 医学のあゆみ 224 Pages: 172-173
  • Peer Reviewed
• [Journal Article] S1P2 receptors mediate inhibition of glioma cell migration through Rho signaling pathways independent of PTEN. (2008)
  • Author(s): Malchinkhuu, E., Sato, K., Maehama, T., Mogi, C., Tomura, H., Ishiuchi, S., Yoshimoto, Y., Kurose, H., Okajima, F.
  • Journal Title: Biochem. Biophys. Res. Commun. 366 Pages: 963-968
  • Peer Reviewed
• [Journal Article] PTEN: its deregulation and tumorigenesis. (2007)
  • Author(s): Maehama, T.
  • Journal Title: Biol. Pharm. Bull. 30 Pages: 1624-1627
  • Peer Reviewed
• [Presentation] 共培養系を用いた高効率なPrPSc伝播実験系の確立 (2009)
  • Author(s): 田中正彦、原英之、萩原健一、花田賢太郎、仁科博史、前濱朝彦
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2009-10-24
• [Presentation] Identification of a Ras-family GTPase as an indispensable signal mediator for nutrient sensing and mTORC1 activation (2008)
  • Author(s): Maehama, T.
  • Organizer: 8th International Conference on Protein Phosphatases
  • Place of Presentation: 群馬県前橋市
  • Year and Date: 2008-11-14