The distinct function of three subtypes of HP1 in mechanisms of genetic inheritance

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Hokkaido University
• Principal Investigator: OBUSE Chikashi Hokkaido University, 大学院・先端生命科学研究院, 教授 (00273855)
• Project Period (FY): 2007 – 2009
• Keywords: 染色体 / ヘテロクロマチン / HP1 / 定量プロテオミクス / 質量分析 / インフォマティクス / クロマチン / 細胞周期

Research Abstract

Heterochromatin protein 1 (HP1) plays a critical role in heterochromatin formation and mitotic progression through interaction with various proteins. In this study, we tried to identify binding proteins of HP1 subtypes, α, β, γ, by proteomic approach. We revealed that 1) all subtypes binds many kinds of protein through a similar way, 2) a protein newly identified as HP1 binding, POGZ, binds to HP1 and regulates HP1 binding of other protein, 3) there are several proteins those can bind to a specific subtype of HP1, those may provide specific characterization of each HP1 subtype.

Research Products

• [Journal Article] Inner Centromere Formation Requires hMis14, a Trident Kinetochore Protein That Specifically Recruits HP1 to Human Chromosomes. (2010)
  • Author(s): Kiyomitsu T., Iwasaki O., Obuse C., Yanagida M.
  • Journal Title: J. Cell Biol. 188 Pages: 791-807
  • Peer Reviewed
• [Journal Article] The E3 ligase TTC3 Facilitates Ubiquintination and Degradation of Phosphorylated Akt. (2009)
  • Author(s): Suizu F., Hiramuki Y., Okumura F., Matsuda M., Okumura J.A., Hirata N., Narita M., Kohno T., Yokota J., Bohgaki M., Obuse C., Hatakeyama S., Obata T., Noguchi M.
  • Journal Title: Dev. Cell 17 Pages: 800-810
  • Peer Reviewed
• [Journal Article] Active Establishment of Centromeric CENP-A Chromatin by RSF Complex. (2009)
  • Author(s): Perpelescu M., Nozaki N., Obuse C., Yang H., Yoda Y.
  • Journal Title: J. Cell Biol. 185 Pages: 397-407
  • Peer Reviewed
• [Journal Article] The Augmin Complex Plays a Critical Role in Spindle Microtubule Generation for Mitotic Progression and Cytokinesis in Human Cells. (2009)
  • Author(s): Uehara R., Nozawa R., Tomioka A., Petry S., Vale R.D., Obuse C., Goshima G.
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 6998-7003
  • Peer Reviewed
• [Journal Article] Human Blinkin/AF15q14 Is Required for Chromosome Alignment and the Mitotic Checkpoint through Direct Interaction with Bub1 and BubR1. (2007)
  • Author(s): Kiyomitsu T., Obuse C., Yanagida M.
  • Journal Title: Dev. Cell 13 Pages: 663-676
  • Peer Reviewed
• [Journal Article] Alpha-Klotho as a Regulator of Calcium Homeostasis. (2007)
  • Author(s): Imura A., Tsuji Y., Murata M., Maeda R., Kubota K., Iwano A., Obuse C., Togashi K., Tominaga M., Kita N., Tomiyama K., Iijima J., Nabeshima Y., Fujioka M., Asato R., Tanaka S., Kojima K., Ito J., Nozaki K., Hashimoto N., Ito T., Nishio T., Uchiyama T., FuImura A.
  • Journal Title: Science 316 Pages: 1615-1618
  • Peer Reviewed
• [Journal Article] Helicobacter Pylori CagA Targets PAR-1/MARK Kinase to Disrupt Epithelial Cell Polarity. (2007)
  • Author(s): Saadat I., Higashi H., Obuse C., Umeda M., Kamiya M.K., Saito Y., Lu H., Ohnishi N., Azuma T., Suzuki A., Ohno S., Hatakeyama M.
  • Journal Title: Nature 447 Pages: 330-333
  • Peer Reviewed
• [Journal Article] Priming of Centromere for CENP-A Recruitment by Human hMis18α, βand M18BP. (2007)
  • Author(s): Fujita Y., Hayashi T., Kiyomitsu T., Toyoda Y., Kokubu A., Obuse C., Yanagida M.
  • Journal Title: Dev. Cell 12 Pages: 17-30
  • Peer Reviewed
• [Journal Article] Human POGZ modulates HP1 dissociation from mitotic chromosome arms through Aurora B activation
  • Author(s): Nozawa R., Nagao K., Masuda H, Iwasaki O., Hirota T., Nozaki N., Kimura H., Obuse C.
  • Journal Title: Nature Cell Biol. (in press)
  • Peer Reviewed
• [Presentation] HP1相互作用因子POGZ/ZNF280EはM期におけるAuroraBキナーゼの染色体腕部からの解離と活性化に寄与する (2010)
  • Author(s): 野澤竜介、長尾恒治、桝田浩孝、小布施力史
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場市
  • Year and Date: 2010-01-20
• [Presentation] 新規HP1結合因子HPB85の機能解析 (2009)
  • Author(s): 位上健太郎、野澤竜介、桝田浩孝、小布施力史
  • Organizer: BMB2009
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] ヘテロクロマチンタンパク室HP1を介したKBAB-ZNFタンパク質ネットワークの解析 (2009)
  • Author(s): 野呂絵美子、野澤竜介、小布施力史
  • Organizer: BMB2009
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] 脱SUMO化活性のある新規HP1結合因子、SENP7の機能解析 (2009)
  • Author(s): 岡田晃明、野澤竜介、小布施力史
  • Organizer: BMB2009
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] プロテオミクスによるヘテロクロマチンの構築とダイナミクスの解明 (2009)
  • Author(s): 野澤竜介、長尾恒治、桝田浩孝、小布施力史
  • Organizer: BMB2009
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] A HP1-interracting ZINC-FINGER protein SHAL5/POGZ/ZNF280E is involved in mitotic chromosome segregation (2009)
  • Author(s): Nozawa, R., Nagao, K., Obuse, C.
  • Organizer: EMBO conference series on nuclear structure anddynamics
  • Place of Presentation: L'lsle sur la Sorgue, France
  • Year and Date: 20090928-20091006
• [Presentation] A HP1-interacting ZINC-FINGER protein POGOZ/ZNF280E is involved in mitotic chromosome segregation Keyston Symposia (2009)
  • Author(s): Nozawa, R., Nagao, K., Obuse, C.
  • Organizer: Chromatin Dynamics and Higher Order Organization
  • Place of Presentation: Idaho
  • Year and Date: 20090225-20090302
• [Presentation] An HP1 binding protein POGZ has pivotal role in mitotic chromosome organization in human cells International mini-symposium on chromosome biology (2009)
  • Author(s): Obuse, C.
  • Organizer: Centromeres to telomeres and the chromatin between
  • Place of Presentation: 札幌市
  • Year and Date: 2009-11-05
• [Presentation] 新規HP1相互作用因子であるジンクフィンガータンパク質POGZ/ZNF280Eは染色体分配に関与する (2008)
  • Author(s): 野澤竜介、長尾恒治、岩崎治、桝田浩孝、小布施力史
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Presentation] A HP1-Interacting zinc-finger protein POGOZ/ZNF280E is involved in mitotic chromosome (2008)
  • Author(s): Nozawa, R., Nagao, K., Masuda, H., Obuse, C.
  • Organizer: CSHL meeting on Dynamic organization of nuclear function
  • Place of Presentation: New York
  • Year and Date: 20080917-20080921
• [Presentation] (2007)プロテオミクスによるヒトヘテロクロマチンタンパク質HP1と相互作用する因子の網羅的な解析. (2007)
  • Author(s): 野澤竜介、長尾恒治、岩崎治、小布施力史
  • Organizer: BMB2007
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] (2007)ヒトヘテロクロマチンタンパク質HP1と相互作用因子のプロテオミクス解析. (2007)
  • Author(s): 野澤竜介、長尾恒治、岩崎治、小布施力史
  • Organizer: 第7回核ダイナミクス研究会
  • Place of Presentation: 札幌
  • Year and Date: 20070925-20070927
• [Presentation] (2007) Identification and characterization of novel human ORC binding proteins (2007)
  • Author(s): Seki, T., Ohta, S., Obuse, C.
  • Organizer: CSHL meeting on Eukaryotic DNA replication & genome maintenance
  • Place of Presentation: New York
  • Year and Date: 20070905-20070909
• [Presentation] (2007) Comprehensive proteomic analyses of human heterochromatin protein 1 (HP1) interacting (2007)
  • Author(s): Nozawa, R., Nagao, K., Iwasaki, O., Obuse, C.
  • Organizer: CSHL meeting on Eukaryotic DNA replication & genome maintenance
  • Place of Presentation: New York
  • Year and Date: 20070905-20070909
• [Book] 分子生物学(「プロテオミクス」、「ゲノミクス」、「インフォーマティクス」の項, 柳柳田充弘・西田栄介・野田亮編:改訂版) (2009)
  • Author(s): 小布施力史
  • Total Pages: 129-133
  • Publisher: 東京化学同人
• [Book] 細胞工学(別冊)(染色体を構築する複合体のプロテオミクス解析. 明日を拓く新次元プロテオミクス) (2009)
  • Author(s): 長尾恒治, 野澤竜介, 小布施力史
  • Total Pages: 81-90
• [Book] 実験医学(増刊)(ヘテロクロマチン結合因子とその機能. 細胞核-遺伝情報制御と疾患) (2009)
  • Author(s): 野澤竜介, 小布施力史
  • Total Pages: 27,123-130
• [Book] タンパク質・核酸・酵素(増刊)(ブリンキン-紡錘体形成チェックポイントと微小管結合に必須なキネトコアタンパク質. 染色体サイクル(正井久雄・升方久夫・釣本敏樹・仁木宏典・篠原彰編) (2009)
  • Author(s): 清光智美, 小布施力史, 柳田充弘
  • Total Pages: 54,421-426
• [Remarks] ホームページ等
  • URL: http://www.lfsci.hokudai.ac.jp/labs/infgen/