Transcriptional regulatory mechanism of Golgi stress response

2008 Fiscal Year Final Research Report

• Project/Area Number: 19370086
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: YOSHIDA Hiderou Kyoto University, 大学院・理学研究科, 准教授 (60378528)
• Co-Investigator(Renkei-kenkyūsha): OKU Masaya 京都大学, 大学院・理学研究科, 博士1年 ; UEMURA Aya 京都大学, 大学院・理学研究科, 修士2年 ; YOKOYAMA Takaaki 京都大学, 理学部, 4年生 ; SUGIURA Shuji 京都大学, 理学部, 4年生 ; TAKEHARA Toshiyuki 京都大学, 理学部, 4年生
• Project Period (FY): 2007 – 2008
• Keywords: ゴルジ体ストレス / 小胞体ストレス / 転写因子 / 糖鎖 / 小胞輸送 / 細胞小器官

Research Abstract

細胞内には様々な細胞小器官が存在し、細胞の機能を分担している。細胞小器官の存在量は細胞の需要に応じてダイナミックに変化するが、その調節機構については明らかではない。本研究では、細胞小器官のひとつであるゴルジ体の存在量の調節機構を解析した結果、制御に関わる転写制御配列と転写制御因子の同定に成功した。本研究成果は、基礎研究のみならずゴルジ体の関与する疾患の病態解明のための基盤となるものである。

Research Products

• [Journal Article] pXBP1(U), a negative regulator of the unfolded protein response activator pXBP1(S), targets ATF6 but not ATF4 in proteasome-mediated degradation (2009)
  • Author(s): *Yoshida, H., Uemura, A. and *Mori, K.
  • Journal Title: Cell Struct. Funct.(*Corresponding author) 34 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Human HRD1 promoter carries a functional unfolded protein response element to which XBP1 but not ATF6 directly binds (2008)
  • Author(s): Yamamoto, K., Suzuki, N., Wada, T., Okada, T., Yoshida, H., Kaufman, R. J., and Mori, K.
  • Journal Title: J. Biochem. 144 Pages: 477-486
  • Peer Reviewed
• [Journal Article] ATF6 is a transcription factor specializing in the regulation of quality control proteins in the endoplasmic reticulum (2008)
  • Author(s): Adachi, Y., Yamamoto, K., Okada, T., Yoshida, H., Harada, A. and Mori, K.
  • Journal Title: Cell Struct. Funct. 33 Pages: 75-89
  • Peer Reviewed
• [Journal Article] Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1 (2007)
  • Author(s): Yamamoto, K., Sato, T., Matsui, T., Sato, M., Okada, T., Yoshida, H., Harada, A. and Mori, K.
  • Journal Title: Dev. Cell 13 Pages: 365-376
  • Peer Reviewed
• [Journal Article] A role of disulfide bridges formed in the lumenal domain of ATF6 in sensing endoplasmic reticulum stress (2007)
  • Author(s): Nadanaka, S., Okada, T., Yoshida, H. and Mori, K.
  • Journal Title: Mol.Cell.Biol 27 Pages: 1027-1043
  • Peer Reviewed
• [Journal Article] ER stress and diseases (2007)
  • Author(s): Yoshida, H.
  • Journal Title: FEBS J. 274 Pages: 630-658
  • Peer Reviewed
• [Journal Article] Unconventional splicing of XBP-1 mRNA in the unfolded protein response (2007)
  • Author(s): Yoshida, H.
  • Journal Title: Antioxid. Redox Signal 9 Pages: 2323-2333
  • Peer Reviewed
• [Presentation] Promoter analysis of target genes of Golgi stress response (2008)
  • Author(s): M. Oku, T.Yokoyama, A. Uemura and H. Yoshida
  • Organizer: Cold Spring Harbor Laboratory Meeting
  • Place of Presentation: Cold Spring Harbor, USA
  • Year and Date: 20080000
• [Presentation] Regulatory factors of a transcription factor XBP1 that controls mammalian unfolded protein response (2008)
  • Author(s): A. Uemura, S. Sugiura, T. Takehara, M. Oku, and H. Yoshida
  • Organizer: 日本分子生物学会年会(第31回)
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Presentation] Target genes of the Golgi stress response in budding yeast (2008)
  • Author(s): T. Takehara, M. Oku, T.Yokoyama, A. Uemura and H. Yoshida
  • Organizer: 日本細胞生物学会大会(第60回)
  • Place of Presentation: 横浜
  • Year and Date: 20080000
• [Presentation] pXBP1(U), a Negative Regulator of the Unfolded Protein Response Activator pXBP1(S), Targets ATF6 but not ATF4 in proteasome-mediated Degradation (2008)
  • Author(s): K. Mori, A. Uemura, H. Yoshida
  • Organizer: 臨床ストレス学会大会(第3回)
  • Place of Presentation: 秋田
  • Year and Date: 20080000
• [Presentation] Stress response to Golgi-disturbing agents (2007)
  • Author(s): M. Oku, T.Yokoyama, A. Uemura and H. Yoshida
  • Organizer: Gordon Research Conference
  • Place of Presentation: Oxford, UK
  • Year and Date: 20070000
• [Presentation] Golgi-disturbing agents enhance expression of factors involved in membrane traffic (2007)
  • Author(s): M. Oku, T. Yokoyama, A. Uemura and H. Yoshida
  • Organizer: International Symposium of Membrane Traffic
  • Place of Presentation: Awaji, Japan
  • Year and Date: 20070000
• [Presentation] ゴルジ体機能阻害によって転写が誘導される遺伝子の検索 (2007)
  • Author(s): H. Yoshida
  • Organizer: 日本分子生物学会年会(第30回)
  • Place of Presentation: 横浜
  • Year and Date: 20070000
• [Presentation] Screening for regulatory factors of mammalian cytoplasmic splicing (2007)
  • Author(s): H. Yoshida
  • Organizer: 日本分子生物学会春期シンポジウム(第7回)
  • Place of Presentation: 淡路島
  • Year and Date: 20070000
• [Book] 「フレームスイッチスプライシング」分子細胞生物学辞典(第2版) (2008)
  • Author(s): 吉田秀郎
  • Publisher: 東京化学同人
• [Remarks]
  • URL: http://www.eonet.ne.jp/~biophysics-kyoto/