Fgf8 signaling for cerebellar differentiation

2008 Fiscal Year Final Research Report

• Project/Area Number: 19370090
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tohoku University
• Principal Investigator: NAKAMURA Harukazu Tohoku University, 大学院・生命科学研究科, 教授 (90079690)
• Research Collaborator: SUZUKI-HIRANO Asuka 東北大学, 大学院・生命科学研究科, PD ; HARADA Hidekiyo 東北大学, 大学院・生命科学研究科, 博士課程後期学生
• Project Period (FY): 2007 – 2008
• Keywords: 形態形成 / 細胞内・細胞間情報伝達

Research Abstract

峡部オーガナイザーFgf8の強いシグナルにより、Ras-ERK経路が活性化されると小脳が分化することを示したが、その活性はすぐに抑制される必要があり、ずっと活性化されると中脳胞の小脳への分化転換は起こらない。Fgf8bとDN-Sprouty2の強制発現の後、DN-Sprouty2の転写をOffにすると中脳胞は小脳へとその運命を変えた。本研究により、小脳が分化するためには、Fgf8bによりRas-ERK経路が活性化され、その後負の制御因子Sprouty2, Sef, MKP3等により、その活性が抑制されることが必要であることが明らかになった。

Research Products

• [Journal Article] R-spondin2 expression in the apical ectodermal ridge is essential for outgrowth and patterning in mouse limb development (2008)
  • Author(s): Aoki M, Kiyonari H, Nakamura H, Okamoto H
  • Journal Title: Development Growth & Differentiation 50 Pages: 85-95
  • Peer Reviewed
• [Journal Article] Electroporation in avian embryos (2008)
  • Author(s): Funahashi J, Nakamura H
  • Journal Title: Methods Mol Biol 461 Pages: 377-382
  • Peer Reviewed
• [Journal Article] Tracing retinal fiber trajectory with a method of transposon-mediated genomic integration in chick embryo (2008)
  • Author(s): Harada H, Takahashi Y, Kawakami K, Ogura T, Nakamura H
  • Journal Title: Dev Growth Differ 50 Pages: 697-702
  • Peer Reviewed
• [Journal Article] Isthmus organizer for mesencephalon and metencephalon (2008)
  • Author(s): Nakamura H, Sato T, Suzuki-Hirano A
  • Journal Title: Dev Growth Differ 50 Suppl 1 Pages: S113-118
  • Peer Reviewed
• [Journal Article] 峡部オーガナイザーと小脳・視蓋形成 (2008)
  • Author(s): 仲村春和・杉山清佳・鈴木-平野明日香
  • Journal Title: 蛋白質核酸酵素 53 Pages: 373-378
• [Journal Article] Cloning and developmental expression of a chick G-protein-coupled receptor SCGPR1 (2007)
  • Author(s): Odani N, Pfaff SL, Nakamura H, Funahashi J.
  • Journal Title: Gene Exp Pattern 7 Pages: 375-380
  • Peer Reviewed
• [Journal Article] 細胞間相互作用を担うシグナル系
  • Author(s): 仲村春和
  • Journal Title: 化学 62 Pages: 23-27
• [Presentation] Pea3/Ets transcription factors for cerebellar differeniation (2008)
  • Author(s): Harada, H., Sato, T., Nakamura, H.
  • Organizer: 41st Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: Tokushima, Japan
  • Year and Date: 20080528-30
• [Presentation] Fgf signaling in tectal polarity formation (2008)
  • Author(s): Harada, H., Tanaka, J., Matsuda, Y., Suzuki-Hirano, A., Nakamura, H.
  • Organizer: Gordon Research Conference 2008 Fibroblast Growth Factors in Development & Disease
  • Place of Presentation: Il Ciocco, Italy
  • Year and Date: 20080302-07
• [Presentation] Regulation of ERK phosphorylation for the MHB formation and cerebellar development (2008)
  • Author(s): Suzuki-Hirano, A., Sato, T., Nakamura, H.
  • Organizer: Gordon Research Conference 2008 Fibroblast Growth Factors in Development & Disease
  • Place of Presentation: Il Ciocco, Italy
  • Year and Date: 20080302-07
• [Presentation] Regulation of the Fgf8-Ras-ERK signaling pathway for cerebellar development (2008)
  • Author(s): Nakamura, H.
  • Organizer: GCOE International Zao Conference
  • Place of Presentation: Miyagi, Japan
  • Year and Date: 20080123-24
• [Presentation] Sequential Regulations of ERK signaling activity and cerebellar development (2007)
  • Author(s): Suzuki-Hirano, A, Sato, T.and Nakamura, H
  • Organizer: International chick meeting
  • Place of Presentation: Barcelona
  • Year and Date: 20070411-14
• [Presentation] Analysis of coactosin, an actin binding protein, in neural crest cell migration (2007)
  • Author(s): Hou, X, Katahira,T, Kimura, J, Nakamura, H
  • Organizer: International chick meeting
  • Place of Presentation: Barcelona
  • Year and Date: 20070411-14
• [Presentation] Establishment of anteroposterior axis of the chick optic tectum by Fgf-Ras/ERK signaling (2007)
  • Author(s): Harada, H, Matsuda, Y, Tanaka, J, Suzuki-Hirano, A, Nakamura, H
  • Organizer: International chick meeting
  • Place of Presentation: Barcelona
  • Year and Date: 20070411-14
• [Book] Electroporation and Sonoporation in Developmental Biology(Tokyo) (2008)
  • Author(s): Nakamura, H.(Editor)
  • Total Pages: 331
  • Publisher: Springer Japan