Analysis of gene regulation and eye development by a novel nuclear protein, Nemp1

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370091
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: TAIRA Masanori The University of Tokyo, 大学院・理学系研究科, 准教授 (60150083)
• Project Period (FY): 2007 – 2009
• Keywords: 初期発生 / 眼形成 / 核内膜蛋白質 / 遺伝子発現調節 / otx2 / rax / BAF / Ran

Research Abstract

We investigated the molecular mechanism of the role of a nuclear membrane protein, Nemp1, in gene regulation during early Xenopus eye development. We found that Nemp1 is localized to the inner nuclear membrane with region B facing the nucleoplasm, and that Nemp1 binds to Barrier-to-Autointegration Factor (BAF) and the GTP form of the small GTPase Ran. Nemp1 inhibits activation of a rax enhancer reporter gene by Otx2 in a region B-dependent manner. These data lead to the possibility that Nemp1 is involved in the expression of the eye-specific gene rax by functioning at the inner nuclear membrane through interactions with BAF and Ran.

Research Products

• [Journal Article] Secreted Frizzled-related proteins enhance the diffusion of Wnt ligands and expand their signalling range (2009)
  • Author(s): Mii, Y., Taira M.
  • Journal Title: Development 136 Pages: 4083-4088
• [Journal Article] The RNA binding protein Mex3b has a fine-tuning system for mRNA regulation in early Xenopus development (2009)
  • Author(s): Takada, H., Kawana, T., Ito, Y., Kikuno, R. F., Mamada, H., Araki, T., Koga, H., Asashima, M., Taira M.
  • Journal Title: Development 136 Pages: 2413-2422
• [Journal Article] Evolutionary origins of blastoporal expression and organizer activity of the vertebrate gastrula organizer gene lhxl and its ancient metazoan paralog lhx3 (2009)
  • Author(s): Yasuoka, Y., Kobayashi, M., Kurokawa, D., Akasaka, K., Saiga, H., Taira M.
  • Journal Title: Development 136 Pages: 2005-2014
• [Journal Article] Involvement of an inner nuclear membrane protein, Nempl, in Xenopus neural development through an interaction with the chromatin protein (2009)
  • Author(s): Mamada, H., Takahashi, N., Taira M.
  • Journal Title: BAF.Dev.Biol 327 Pages: 497-507
• [Journal Article] Inaugural Article: A comparative analysis of frog early development (2007)
  • Author(s): del Pine, E. M., Venegas-Ferrin, M., Romero-Carvajal, A., Montenegro-Larrea, P., Saenz-Ponce, N., Moya, I. M., Alarcon, I., Sudou, N., Yamamoto, S., Taira M.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 11882-11888
• [Journal Article] Recombinant To12 transposase with activity in Xenopus embryos (2007)
  • Author(s): Shibano, T., Taira M., Suetake, I., Kawakami, K., Asashima, M., Tajima, S., Taira M.
  • Journal Title: FEBS Lett 581 Pages: 4333-4336
• [Presentation] Molecular mechanisms of the regulation of rax expression by the inner nuclear membrane protein Nempl in vertebrate eye development (2010)
  • Author(s): T.Shibano, H.Mamada, N.Sudou, F.Hakuno, S.-I.Takahashi, M.Taira
  • Organizer: The 2010 CSHL Symposium on Nuclear Organization & Function
  • Year and Date: 20100602-20100607
• [Presentation] Molecular mechanisms of the regulation of rax expression by the inner nuclear membrane protein Nempl in vertebrate eye development (2009)
  • Author(s): T.Shibano, H.Mamada, F.Hakuno, S.-I.Takahashi , H.Koga, M.Taira
  • Organizer: 第32回分子生物学会年会、(柴野、ワークショップロ頭発表とポスター発表)
  • Place of Presentation: 横浜
  • Year and Date: 20091209-20091212
• [Presentation] Interaction of the inner nuclear membrane protein Nempl with the chromatin protein BAF and the small GTPase Ran in Xenopus embryos (2009)
  • Author(s): T.i Shibano, H.Mamada, F.Hakuno, S.-I.Takahashi , H.Koga, M.Taira
  • Organizer: 日本発生生物学会第42回大会、(柴野、 口頭発表)
  • Place of Presentation: 新潟
  • Year and Date: 20090528-20090531
• [Presentation] 眼の発生に関わる核内膜蛋白質Nemp 1と相互作用する因子の同定 (2008)
  • Author(s): 柴野卓志、儘田博志、伯野史彦、高橋伸一郎、古閑比佐志、平良眞規
  • Organizer: 第31回分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-20081212
• [Presentation] アフリカツメガエル初期眼発生における新規核膜タンパク質Nemp 1の解析 (2007)
  • Author(s): 儘田博志、柴野卓志、伯野史彦、高橋伸一郎、古閑比佐志、平良眞規
  • Organizer: 第30回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20071211-20071215
• [Presentation] Analysis of a novel nuclear envelope protein, Nempl, in early Xenopus eye development (2007)
  • Author(s): H.Mamada, T.Shibano, F.Hakuno, S.-I.Takahashi, H.Koga, M.Taira
  • Organizer: International Symposium on Advanced Functional Genomics
  • Place of Presentation: Kazusa
  • Year and Date: 20071011-20071012
• [Remarks] ホームページ
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/1mb/index.php