Elucidation of the molecular system of skeletal formation using mouse andmedaka mutants of organogenesis

2008 Fiscal Year Final Research Report

• Project/Area Number: 19370093
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KUDO Akira Tokyo Institute of Technology, 大学院・生命理工学研究科, 教授 (70178002)
• Project Period (FY): 2007 – 2008
• Keywords: トランスジェニックメダカ / メダカ突然変異体 / 骨格形成 / 破骨 / 破骨

Research Abstract

種々のメダカ骨格異常変異体の原因遺伝子が解明され、脊椎動物共通の骨格形成システムが明らかになった。またメダカ変異体は、ヒト疾患モデルとして重要な実験動物であり、今後もその原因解明に利用できる。小型魚類でメカニカルストレス依存性の筋間中隔形成に働き、細胞外マトリックス蛋白であるぺリオスチンは、そのノックアウトマウスの解析から、切歯萌出、心筋梗塞時の心筋再生に機能することがわかった。従って、小型魚類とマウスを統合的に解析することによって、ぺリオスチンがメカニカルストレスによる組織再生に機能する分子であることが明らかになった。

Research Products

• [Journal Article] Brpf1, a subunit of the MOZ histone acetyl transferase complex, maintains expression of anterior and posterior Hox genes for proper patterning of craniofacial and caudal skeletons (2009)
  • Author(s): Hibiya, K., Katsumoto, T., Kondo, T., Kitabayashi, I. and Kudo, A.
  • Journal Title: Dev. Biol.(Epub)
• [Journal Article] Reduced proliferative activity of primary POMGnT1-null myoblasts in vitro (2009)
  • Author(s): Miyagoe-Suzuki, Y., Masubuchi, N., Miyamoto, K., Wada, MR. Yuasa, S., Saito, F., Matsumura, K., Kanesaki, H., Kudo, A., Manya, H., Endo, T. and Takeda, S.
  • Journal Title: Mech. Dev. 126 Pages: 107-116
• [Journal Article] Peiostin is expressed in pericryptal fibroblasts and cancer-associated fibroblasts in the colon. Journal of Histochemistry & Cytochemistry (2009)
  • Author(s): Kikuchi,Y., kashima, T.G., Nishiyama,T., Shimazu,K., Morishita,Y., Shimazaki,M., Kii,I., Horie,H., Nagai,H., Kudo, A.. And Fukayama,M.
  • Journal Title: 56 (8) Pages: 753-764
• [Journal Article] Gene expression and functional analysis of zebrafish larval fin fold regeneration (2009)
  • Author(s): Yoshinari,N., Ishida,T., Kudo, A. and Kawakami.A.
  • Journal Title: Dev. Bio. 325 Pages: 71-81
• [Journal Article] Periostin, a novel marker of intramembranous ossification, is ecpressed in fibrous dysplasia and in c-Fos-overexpressing bon lesions (2009)
  • Author(s): Kashima,T.G., Nishiyama,T., Shimazu,K., Shimazaki,M., Kii,I., Grigoriadis,A.E., Fukayama,M., and Kudo, A
  • Journal Title: Human Pathology 40 Pages: 226-237
• [Journal Article] Mutation in the abcb7 gene causes abnormal iron and fatty acid metabolism in developing medaka fish (2008)
  • Author(s): iyake, A., Higashijima, S., Kobayashi, D., Narita, T., Jindo, T., Davin H. E. Setiamarga, D.H.E., Ohisa, S., Orihara, N., Hibiya, K., Konno, S., Sakaguchi, S., Horie, K., Imai, Y., Naruse, K., Kudo, A. and Takeda, H.
  • Journal Title: Dev. Growth Differ 50 Pages: 703-716
• [Journal Article] Periostin deposition in the stroma of invasive and intraductal neoplasms of the pancreas (2008)
  • Author(s): Fukushima, N., Kikuchi, Y., Nishiyama, T., Kudo, A., and Fukayama, M.
  • Journal Title: Mod. Pathol 21 Pages: 1044-1053
• [Journal Article] The medaka FoxP2, a homologue of human language gene FOXP2, has a diverged structure and function (2008)
  • Author(s): Itakura,T., Chandra, A., Yang, Z., Xue X.D., Wang, B., Kimura, W., Hikosaka, K., Inohaya, K., Kudo, A., Uezato, T., and Miura, N.
  • Journal Title: J. Biochem 143 Pages: 407-416
• [Journal Article] Function of Pax1 and Pax9 in the sclerotome of medaka fish (2008)
  • Author(s): Mise, T., Iijima, M., Inohaya, K., Kudo, A. and Wada, H.
  • Journal Title: Genesis 46 Pages: 185-192
• [Journal Article] Impaired capsule formation of tumors in periostin-null mice (2008)
  • Author(s): Shimazaki, M. and Kudo, A.
  • Journal Title: Biochem. Biophys. Res. Com. 342 Pages: 766-772
• [Journal Article] Periostin is essential for cardiac healing after acute myocardial infarction (2008)
  • Author(s): Shimazaki, M., Nakamura, K., Kii, I., Kashima, T., Amizuka, N., Li, M., Saito, M., Fukuda, K., Nishiyama, T., Kitajima, S., Saga, Y., Fukayama, M., Sata, M., and Kudo, A.
  • Journal Title: J. Exp. Med. 205 Pages: 295-303
• [Journal Article] Migration of mesenchymal cell fated to blastema is necessary for fish fin regeneration (2008)
  • Author(s): Nakatani, Y., Nishidate, M., Fujita, M., Kawakami, A. and Kudo, A.
  • Journal Title: Dev. Growth Differ 50 Pages: 71-83
• [Journal Article] Expression of marker genes during otolith development in medaka (2008)
  • Author(s): Nemoto, Y., Chatani, M., Inohaya, K., Hiraki, Y. and Kudo, A.
  • Journal Title: Gene Exp. Patterns 8 Pages: 92-95
• [Journal Article] Bone marrow stromal cell lines having high potential for osteoclast-supporting activity express PPAR・1 and show a high potential for differentiation into adipocytes (2008)
  • Author(s): Takagi, K. and Kudo, A.
  • Journal Title: J Bone Miner. Metab. 26 Pages: 13-23
• [Journal Article] Histochemical examinations on cortical bone regeneration induced by thermoplastic bioresorbable plates to bone defects on rat calvariae (2007)
  • Author(s): Kojima, T., Freitas, P.H.L., Ubaidus, S., Suzuki, A., Li, M., Yoshizawa, M., Oda, K., Maeda, T., Kudo, A., Saito, C. and Amizuka, N.
  • Journal Title: Biomedical Res. 28 Pages: 219-229
• [Journal Article] The teleost intervertebral region acts as a growth center of the centrum : In vivo visualization of osteoblasts and their progenitors in transgenic fish (2007)
  • Author(s): Inohaya, K., Takano, Y. and Kudo, A.
  • Journal Title: Developmental Dynamics 236 Pages: 3031-3046
• [Journal Article] Identification of novel markers expressed during fin regeneration by microarray analysis in Medaka fish (2007)
  • Author(s): Nishidate, M., Nakatani, Y., Kudo, A. and Kawakami, A.
  • Journal Title: Developmental Dynamics 236 Pages: 2685-2693
• [Journal Article] Histological examinations on the bone regeneration achieved by combining grafting with hydroxyapatite and thermoplastic bioresorbable plates (2007)
  • Author(s): Kojima, T., Amizuka, N., Suzuki, A., Freitas, P.H., Yoshizawa, M., Kudo, A., Saito, C. and Maeda, T.
  • Journal Title: J Bone Miner. Metab. 25 Pages: 361-373
• [Journal Article] Dual effects of the membrane-anchored MMP regulator RECK on chondrogenic differentiation of ATDC5 cells (2007)
  • Author(s): Kondo, S., Shukunami, C., Morioka, Y., Matsumoto, N., Takahashi, R., Oh, J., Atsumi, T., Umezawa, A., Kudo, A., Kitamura, H., Hiraki, Y. and Noda, M.
  • Journal Title: J. Cell Sci. 120 Pages: 849-857
• [Journal Article] Cellular and molecular processes of regeneration, with special emphasis on fish fins (review) Dev. (2007)
  • Author(s): Nakatani, Y., Kawakami, A. and Kudo, A.
  • Journal Title: Growth Differ 49 Pages: 145-154
• [Journal Article] GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen (2007)
  • Author(s): Moriyama, A., Kii, I., Sunabori, T., Kurihara, S., Takayama, I. Shimazaki, M., Tanabe, H., Oginuma, M., Fukayama, M., Matsuzaki, Y., Saga, Y., and Kudo, A.
  • Journal Title: Genesis 45 Pages: 90-100
• [Journal Article] Multinucleate osteoclasts in medaka as evidence of active bone remodeling (2007)
  • Author(s): Nemoto, Y., Higuchi, K., Baba, O., Kudo, A. and Takano, Y
  • Journal Title: Bone^+(+ : equal correspondence) 40 Pages: 399-408
• [Presentation] 心室の拍動不全メダカ変異体hozuki(hoz)におけるストレスに依存した心筋細胞増殖機構の解析BMB2008 (2008)
  • Author(s): 種田悠佑、今野さやか、牧野伸司、福田恵一、工藤明、川上厚志
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-12
• [Presentation] 神経型一酸化窒素合成酵素はAktシグナルを介して筋肥大の進行を制御しているBMB2008 (2008)
  • Author(s): 伊藤尚基、Ampong, B.N., 工藤明、鈴木友子武田伸一
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20081209-12
• [Presentation] In-vivo imaging for osteoclasts in medaka, showing the evidence of bone modeling (2008)
  • Author(s): Chatani, M., Inohaya, K., Kudo, A.
  • Organizer: The 8th international meeting on zebrafish development and genetics
  • Place of Presentation: Madison, US
  • Year and Date: 20080625-29
• [Presentation] Characterization of the medaka mutant which displays abnormalities in hematopoiesis (2008)
  • Author(s): Moriyama, A., Ohisa, Satoshi, Kudo, A.
  • Organizer: The 8^<th>international meeting on zebrafish development and genetics
  • Place of Presentation: Madison, USA
  • Year and Date: 20080625-29
• [Presentation] Characterization of the medaka mutant which displays abnormalities in vertebral body (2008)
  • Author(s): Ohisa, S., Inohaya, K., Kawakami, A., Kudo, A.
  • Organizer: The 8^<th>international meeting on zebrafish development and genetics
  • Place of Presentation: Madison, USA
  • Year and Date: 20080625-29
• [Presentation] Positional cloning rebeals the brpf1 locus in the medaka mutant which displays craniofacial homeosis (2008)
  • Author(s): Hibiya, K., Inohaya, K., Kawakami, A., Kudo, A.
  • Organizer: The 8^<th>international meeting on zebrafish development and genetics
  • Place of Presentation: Madison, USA
  • Year and Date: 20080625-29
• [Presentation] Identification and functional analysis of regeneration-associated molecules using the fin-fold regeneration model in juvenile zebrafish (2008)
  • Author(s): Kawakami, A., Ishida, T., Yoshinari, N., Kudo, A.
  • Organizer: The 8^<th>international meeting on zebrafish development and genetics
  • Place of Presentation: Madison, USA
  • Year and Date: 20080625-29
• [Presentation] Vertebral bone formation inMdeaka メダカ脊椎骨形成 (2008)
  • Author(s): 工藤明
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 20080528-30
• [Presentation] Characterization of the medaka mutant which displays abnormalities in vertebral body (2008)
  • Author(s): Satoshi Ohisa, Keiji Inohaya, Atsushi Kawakami, Yoshiro Takano, Akira Kudo
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 徳島
  • Year and Date: 20080528-30
• [Presentation] Gene expression and functional analysis of zebrafish larval fin-fold regenerationゼブラフィッシュ正中膜ひれ再生における遺伝子プロファイリングと関連分子機能の解析 (2008)
  • Author(s): Takashi Ishida, Nozomi Yoshinari, Akira Kudo, Atsushi Kawakami
  • Organizer: 第41回日本発生生物学会
  • Place of Presentation: 岡崎
  • Year and Date: 20080528-30
• [Presentation] Analyses by mutants and bone specific transgenic Medaka (2008)
  • Author(s): Kudo, A.Bone formation in Medaka
  • Organizer: The 54^<th>NIBB Conference
  • Place of Presentation: Durham, USA
  • Year and Date: 20080130-0203
• [Presentation] Bone formation in Medaka : Analyses by mutants and bone specific transgenic Medaka (2008)
  • Author(s): Kudo, A
  • Organizer: The fourth aquatic animal models of human disease conference
  • Place of Presentation: Durham, USA
  • Year and Date: 20080130-0203
• [Presentation] Periostin is essential for cardiac healing after acute myocardial infarction (2008)
  • Author(s): Shimazaki, M., Nakamura, K., Kii, I., Sata, M. and Kudo, A.
  • Organizer: Keystone symposia on cellular biology
  • Place of Presentation: Vancouver, USA
  • Year and Date: 20080115-20
• [Presentation] 小型魚類を用いた骨代謝研究 宇宙基礎医学研究に用いるべき最適なモデル生物に関するワークショップ (2008)
  • Author(s): 工藤明、高野吉郎
  • Organizer: 第15回宇宙医学研究推進分科会
  • Place of Presentation: 東京
  • Year and Date: 2008-01-11
• [Presentation] メダカの世界 : ゲノム・再生・宇宙 (2007)
  • Author(s): 工藤明
  • Organizer: ゲノムひろば2007
  • Place of Presentation: 大阪
  • Year and Date: 20071013-14
• [Presentation] 骨・歯の力学的負荷に機能する再生蛋白、ペリオスチン (2007)
  • Author(s): 工藤明
  • Organizer: 第49回歯科基礎医学会学術大会ならびに総会
  • Place of Presentation: 北海道大学
  • Year and Date: 20070829-31
• [Presentation] 疾患モデルとしてのメダカ : 突然変異体の解析とアレイによるヒレ再生の解析 (2007)
  • Author(s): 工藤明
  • Organizer: 第13回動物遺伝育種シンポジウム「動物ゲノム解析と新たな家畜育種戦略」
  • Place of Presentation: つくば
  • Year and Date: 2007-11-25
• [Book] 心筋梗塞を修復するタンパク質、ペリオスチンの機能解明、細胞工学27(06) (2008)
  • Author(s): 島崎雅司、工藤明
  • Total Pages: 598-599
  • Publisher: 秀潤社
• [Book] ペリオスチンと歯根膜・骨外膜、Clinical Calcium(Vo.17, No.2) (2007)
  • Author(s): 喜井勲、工藤明
  • Total Pages: 62-68
  • Publisher: 医薬ジャーナル社
• [Patent(Industrial Property Rights)] 心筋壊死を伴う疾患の治療薬及び検査薬 (2008)
  • Inventor(s): 工藤明
  • Industrial Property Rights Holder: 東京工業大学・三菱メディエンス
  • Industrial Property Number: 特許,特願2008-187851
  • Filing Date: 2008-07-18