The elucidation of the physiological polymorphism related to arteriosclerosis by the approach method from genetic and acquired factor

2009 Fiscal Year Final Research Report

• Project/Area Number: 19370104
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 生理人類学
• Research Institution: Nagasaki University
• Principal Investigator: MAEDA Takahiro Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (40284674)
• Co-Investigator(Kenkyū-buntansha): NAKASHIMA Kenichiro 長崎大学, 大学院・医歯薬学総合研究科, 教授 (30039656) ; AOYAGI Kiyoshi 長崎大学, 大学院・医歯薬学総合研究科, 教授 (80295071) ; TAKAMURA Noboru 長崎大学, 大学院・医歯薬学総合研究科, 教授 (30295068) ; YAMASAKI Hironori 長崎大学, 保健・医療推進センター, 准教授 (40346953) ; KUSANO Yosuke 長崎ウエスレヤン大学, 現代社会学部, 教授 (70325637)
• Project Period (FY): 2007 – 2009
• Keywords: 生理的多型性

Research Abstract

We conducted the investigation study related to arteriosclerosis in small remote islands with a population of less than 1,000 and large island with a population of about 40,000 in Goto islands, where the environmental factor was considered to be different. Comparing 205 inhabitants in small islands with 2,520 inhabitants in large island after adjustment for age and sex, plasma homocysteine concentration and carotid intima-media thickness was significantly higher in large island, and plasma folic acid concentration was significantly higher in small island. Because there was not a difference by areas regarding the genetic factor related to the metabolism of folic acid, it was suggested that the physiological polymorphism with acquired factor existed.

Research Products

• [Journal Article] Percentage body fat assessed by bioelectrical impedance analysis as a new health index for rural areas in the Asia-Pacific region. (2009)
  • Author(s): Minematsu K, Kaneko Y, Nakazato M, Maeda T, Christopher NJ, Yoda T, Goto K, Takamura N, Mizota T
  • Journal Title: Acta Med Nagasaki 54 Pages: 39-43
  • Peer Reviewed
• [Journal Article] A validated HPLC-fluorescence method with a semi-micro column for routine determination of homocysteine, cysteine and cysteamine, and the relation between the thiol derivatives in normal human plasma. (2009)
  • Author(s): Ichinose S, Nakamura M, Maeda M, Ikeda R, Wada M, Nakazato M, Ohba Y, Takamura N, Maeda T, Aoyagi K, Nakashima K
  • Journal Title: Biomed Chromatogr 23 Pages: 935-939
  • Peer Reviewed
• [Journal Article] Relationship between periodontal status and HbA1c in non-diabetics. (2009)
  • Author(s): Hayashida H, Kawasaki K, Yoshimura A, Kitamura M, Furugen R, Nakazato M, Takamura N, Hara Y, Maeda T, Saito T
  • Journal Title: J Publ Health Dent 69 Pages: 204-206
  • Peer Reviewed
• [Journal Article] Thyroid function is associated with carotid intima-media thickness in euthyroid subjects. (2009)
  • Author(s): Takamura N, Akilzhanova A, Hayashida N, Kadota K, Yamasaki H, Usa T, Nakazato M, Maeda T, Ozono Y, Aoyagi K, Kadota K
  • Journal Title: Atherosclerosis 204 Pages: e77-e81
  • Peer Reviewed
• [Journal Article] Availability of cardio-ankle vascular index (CAVI) as a screening tool for atherosclerosis. (2008)
  • Author(s): Takamura N, Aoyagi K, Yamasaki H, Usa T, Nakazato M, Maeda T, Wada M, Nakashima K, Ishibashi K,, Abe K, Takeshima F, Ozono Y
  • Journal Title: Circ J 72 Pages: 304-308
  • Peer Reviewed
• [Journal Article] Multimers and adiponectin gene 276G>T polymorphism in the Japanese population residing in rural areas. (2007)
  • Author(s): Takamura N, Aoyagi K, Yamasaki H, Abiru N, Nakazato M, Kamihira S, Maeda T
  • Journal Title: Clin Chem Lab Med 45 Pages: 1457-1463
  • Peer Reviewed
• [Journal Article] Determinants of plasma homocysteine levels and carotid intima-media thickness in Japanese. (2007)
  • Author(s): Takamura N, Abe Y, Nakazato M, Maeda T, Wada M, Nakashima K, Kusano Y, Aoyagi K, Mori S, Sugahara K, Uemura A, Yamada Y, Uzihara K, Hayashida H, Ideguchi T, Ishibashi K, Nakazato M
  • Journal Title: Asia Pac J Clin Nutr 16 Pages: 698-703
  • Peer Reviewed
• [Journal Article] The possibility of a valuable resource of circulating DNA for single nucleotide polymorphisms genotyping: the application of a rapid and a simple polymerase chain reaction with melting curve analysis for methylentetrahydrofolate reductase polymorphisms. (2007)
  • Author(s): Maeda T, Takamura N, Kamihira S
  • Journal Title: Laboratory Hematology 13 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Evaluation of cardiovascular risk factors and related clinical markers in healthy young Japanese adults. (2007)
  • Author(s): Yagura C, Takamura N, Kadota K, Nagazumi T, Morishita Y, Nakazato M, Maeda T, Kusano Y, Abe Y, Aoyagi K
  • Journal Title: Clin Chem Lab Med 45 Pages: 220-225
  • Peer Reviewed
• [Presentation] ルテニウム錯体化学発光を用いる新規葉酸定量法の開発に関する基礎的検討 (2010)
  • Author(s): 一山公佑, 池田理恵, 和田光弘, 前田隆浩, 中島憲一郎
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-28
• [Presentation] Urinary cystatin C as a biomarker for detecting early renal dysfunction in the general populations. (2009)
  • Author(s): Obata Y, Furusu A, Yamashita H, Nishino T, Nakazato M, Miyazaki M, Takamura N, Maeda T, Ozono Y, Kohno S
  • Organizer: World Congress of Nephrology 2009
  • Place of Presentation: Milan, Italy
  • Year and Date: 20090522-20090526
• [Presentation] Cystatin C and the risk of atherosclerosis among asymptomatic adults. (2009)
  • Author(s): Yamashita H, Furusu A, Nishino T, Obata Y, Nakazato M, Miyazaki M, Harada T, Ozono Y, Kohno S
  • Organizer: World Congress of Nephrology 2009
  • Place of Presentation: Milan, Italy
  • Year and Date: 20090522-20090526
• [Presentation] 健常人血漿チオール化合物の定量とその解析 (2009)
  • Author(s): 前田麻由, 中島真裕美, 池田理恵, 和田光弘, 中里未央, 前田隆浩, 高村昇, 青柳潔, 中島憲一郎
  • Organizer: 第49回日本臨床化学会
  • Place of Presentation: 長崎
  • Year and Date: 2009-09-18
• [Presentation] 離島住民における赤血球中葉酸とホモシステインとの関連 (2009)
  • Author(s): 中里未央, 草野洋介, 高村昇, 中島健一郎, 青柳潔, 大園義之, 前田隆浩
  • Organizer: 第60回日本生理人類学会
  • Place of Presentation: 北海道
  • Year and Date: 2009-06-06
• [Presentation] 五島地区の健診者における血清シスタチンCと動脈硬化の関連 (2009)
  • Author(s): 山下裕, 古巣朗, 西野友哉, 小畑陽子, 中里未央, 高村昇, 前田隆浩, 大園恵幸, 河野茂
  • Organizer: 第52回日本腎臓病学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-04
• [Presentation] 五島地区健診受診者における尿中シスタチンCと腎虚血マーカー(FEMg)との関連の検討 (2009)
  • Author(s): 山下裕, 古巣朗, 西野友哉, 小畑陽子, 中里未央, 宮崎正信, 高村昇, 前田隆浩, 大園恵幸, 河野茂
  • Organizer: 第52回日本腎臓病学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-06-03
• [Presentation] :本邦における赤血球中葉酸濃度と血清ホモシステイン、動脈硬化との関連 (2009)
  • Author(s): 中里未央, 坂本大地, 高村昇, 門田耕一郎, 中島憲一郎, 青柳潔, 大園恵幸, 山崎浩則, 前田隆浩, 田村庸信, Kelley Johnston, 中尾郁子, 吉谷清光
  • Organizer: 第17回日本総合診療医学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-01
• [Presentation] 一般住民における潜在性甲状腺機能異常症の頻度とTSH正常者におけるfT3・TSHと動脈硬化との関連 (2009)
  • Author(s): 中里未央, 坂本大地, 高村昇, 門田耕一郎, 大園恵幸, 青柳潔, 前田隆浩, 中尾郁子, 吉谷清光
  • Organizer: 第17回日本総合診療医学会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-01
• [Presentation] he association between plasma homocysteine concentration and atherosclerosis in the Japanese population residing in remote islands. (2008)
  • Author(s): Nakazato M, Shimokawa I, Takamura N, Aoyagi K, Nakashima K, Ozono Y, Maeda T
  • Organizer: The Japan-Korea Joint Seminar (AACL 2008; Asian Aging Core for Longevity 2008)
  • Place of Presentation: Huis Ten Bosch in Nagasaki, Japan
  • Year and Date: 20080904-20080906
• [Presentation] Physiological polymorphism of arteriosclerosis on a remote Japanese island. (2008)
  • Author(s): Kusano Y, Nakazato M, Ishibashi K, Takamura N, Nakashima K, Yamasaki H, Oozono Y, Kuwahara K, Nakamura H, Aoyagi K, Maeda T
  • Organizer: 9th International Congress of Physiological Anthropology
  • Place of Presentation: Delft, The Netherlands
  • Year and Date: 20080822-20080826
• [Presentation] 蛍光誘導体化試薬SBD-Fを用いた血漿中チオール化合物のHPLC-FL定量とそのバリデーション評価 (2008)
  • Author(s): 前田麻由, 中村真裕美, 和田光弘, 中里未央, 前田隆浩, 高村昇, 青柳潔, 中島憲一郎
  • Organizer: 第48回臨床化学会
  • Place of Presentation: 静岡
  • Year and Date: 2008-08-29
• [Presentation] 離島住民におけるアディポネクチン血中濃度と遺伝子多型 (2008)
  • Author(s): 草野洋介, 石橋純美, 高村昇, 青柳潔, 山崎浩則, 中里未央, 前田隆浩
  • Organizer: 第58回日本生理人類学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-06-07
• [Presentation] 小離島と大離島における、Adiponectin 276G>T遺伝子多型とAdiponectin分画 (2008)
  • Author(s): 中里未央, 石橋純美, 山崎浩則, 高村昇, 瀬尾幸, 青柳潔, 大園恵幸, 前田隆浩
  • Organizer: 第16回日本総合診療医学会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-08
• [Presentation] 長崎県小離島における栄養調査～健康調査との関連から～ (2007)
  • Author(s): 渡辺美和, 中里未央, 原和子, 前田隆浩, 川井光子
  • Organizer: 第54回日本栄養改善学会
  • Place of Presentation: 長崎
  • Year and Date: 2007-09-20
• [Presentation] MTHFR遺伝子多型による血中ホモシステイン濃度の変化が動脈硬化の生理的多型に及ぼす影響～島嶼と本土の比較～ (2007)
  • Author(s): 草野洋介, 中里未央, 高村昇, 中島憲一郎, 青柳潔, 大園恵幸, 前田隆浩
  • Organizer: 第56回日本生理人類学会
  • Place of Presentation: 秋田
  • Year and Date: 2007-07-17
• [Presentation] An association between plasma homocysteine concentration and methylenetetrahydrofolate reductase gene C677T polymorphism on a remote island. (2007)
  • Author(s): Nakazato M, Kadota K, Takamura N, Sekitani M, Yamasaki H, Mukae H, Nakashima K, Aoyagi K, Maeda T
  • Organizer: 6th Conference on Homocysteine Metabolism, World Congress on Hyperhomocysteinemia
  • Place of Presentation: Saarbruecken, Germany
  • Year and Date: 2007-06-08