2009 Fiscal Year Final Research Report
Drought tolerance in apple seedlings through abscisic acid (ABA) regulation
| Project/Area Number |
19380025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Chiba University |
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Principal Investigator |
KONDO Satoru Chiba University, 大学院・園芸学研究科, 准教授 (70264918)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Nobuhiro 京都大学, 大学院・農学研究科, 教授 (00165151)
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| Co-Investigator(Renkei-kenkyūsha) |
SUGAYA Sumiko 筑波大学, 大学院・生命科学研究科, 教授 (90302372)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 果樹 |
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| Research Abstract |
In this study, we examined the effect of an inhibitor of ABA 8'-hydroxylase (AHI) on drought tolerance in apple seedlings (Malus domestica Borkh.). ABA metabolism, stomatal aperture, water potential, and radical scavenging activity in leaves were all investigated under drought conditions. The result of our observation had shown that the AHI application to seedlings greatly decreased the stomatal aperture in the leaves as in contrast to untreated control samples.
However, watering of AHI treated plants re-increased it to the original level. In addition, water potential in AHI-treated leaves remained higher than the one measured in untreated-leaves during the exposure to drought. Drought conditions increased ABA concentrations in both, AHI treated-plants and untreated-plant specimens. Notably, ABA concentrations in AHI-treated leaves were higher than those in untreated-leaves under drought conditions. Although the expression of 9-cis-epoxycarotenoid dioxigenase (MdNCED) genes increased with days of drought stress treatment, in AHI-treated leaves the expression of those genes was significantly lower than in untreated control samples.
These results suggest that the prompt stomatal closure through the regulation of ABA 8'-hydroxylase may be required for the plants to survive under relatively long drought conditions.
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[Journal Article] Abscinazole-F1, a conformationally restricted analogue of the plant growth retardant uniconazole and an inhibitor of ABA8'-hydroxylase CYP707A with no growth-retardant effect2009
Author(s)
Y. Todoroki, K. Kobayashi, M. Shirakura, H. Aoyama, K. Takatori, H. Nimiteatkai, M. Jin, S. Hiramatsu, K. Ueno, S. Kondo, M. Mizutani, N. Hirai
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Journal Title
Bioorganic & Medical Chemistry 17
Pages: 6620-6630
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