2009 Fiscal Year Final Research Report
Functional analysis of molecular complex composed by CMV-resistance protein RCY1 and other host proteins
| Project/Area Number |
19380026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Tohoku University |
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Principal Investigator |
TAKAHASHI Hideki Tohoku University, 大学院・農学研究科, 准教授 (20197164)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Nobuyuki 岩手大学, 農学部, 教授 (40191556)
HASE Shu 東北大学, 大学院・農学研究科, 助教 (10261497)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ウイルス抵抗性 / キュウリモザイクウイルス / 病害抵抗性遺伝子 / NB-LRRタンパク質 |
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| Research Abstract |
Resistance gene to CMV(Y), RCY1, encodes CC-NBS-LRR-containing protein. In this study, we analyzed the molecular aspect of a complex composed by RCY1 and other host proteins. In conclusion, (1) RCY1-conferred resistance to CMV(Y) required three structural domains : CC, NBS and LRR in RCY1 protein, (2) HR-cell death was induced by Agrobacterium-mediated transient expression of RCY1, but co-expression of all combination of CC, NBS and LRR domain proteins in plant cell did not induce not only the cell death but also molecular interaction among them, indicating that three domains are not able to act trans-complimentarily, (3) A protein with molecular weight of 43kDa and a NTPase containing 'GHKL'ATPase motif were identified as a RCY1-interacting proteins. These finding will give a new insight for understanding the function of RCY1 protein conferring the resistance to CMV(Y).
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Author(s)
Aoki K., Yano Y., Suzuki A., Kawamura S.,Sakurai N., Suda K., Kurabayashi A., Suzuki T., Tsugane T., Watanabe M., Ooga K.,Torii M., Narita T., Shin-i T., Kohara Y.,Yamamoto N., Takahashi H., Watanabe Y.,Egusa M., Kodama M., Ichinose Y., Kilcuchi M., Fukushima S., Okabe A., Arie T.,Sato Y., Yazawa K., Satoh S., Omura T., Ezura H., Shibata D.
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Journal Title
BMC Genomics 11
Pages: 210
Peer Reviewed
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Author(s)
Igarashi A., Yamagata K., Sugai T., Takahashi Y., Sugawara E., Tamura A., Yaegashi H., Yamagishi N., Takahashi T., Isogai M., Iakahashi H., Ybshikawa N
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Arabidopsis Arabidopsis thaliana cucurbits, and legumes. Virology 386
Pages: 407-416
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[Journal Article] High-level expression of a virus resistance gene2008
Author(s)
Sekine K-T., Kawakami S., Kubota M., Ichinose Y., Shah J., Kang H-G., Klessig D.F., Takahashi H.
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Journal Title
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