Molecular analysis of the regulation mechanism of systemic necrosis development in Arabidopsis thaliana upon bromovirus infection

2009 Fiscal Year Final Research Report

• Project/Area Number: 19380027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyoto University
• Principal Investigator: MISE Kazuyuki Kyoto University, 大学院・農学研究科, 准教授 (90209776)
• Project Period (FY): 2007 – 2009
• Keywords: 感染生理 / 遺伝学 / 遺伝子 / ウイルス / 植物 / 病理

Research Abstract

Viruses infect plants and cause various types of symptoms. In this study, we analyzed the regulation mechanism of systemic necrosis development in Arabidopsis thaliana upon bromovirus infection. Genetic mapping and complementation tests in mutant plants revealed that two adjacent "resistance genes" are essential determinants for symptom development and function cooperatively. Mutational analyses suggest that systemic necrosis development in this virus-plant system is a form of weak and delayed resistance response accompanying hypersensitive reaction-like cell death.

Research Products

• [Journal Article] Identification and characterization of the 480 kDa template-specific RNA-dependent RNA polymerase complex of Red clover necrotic mosaic virus. (2010)
  • Author(s): Mine, A., Takeda, A., Taniguchi, T., Taniguchi, H., Kaido, M., Mise, K.,, Okuno, T.
  • Journal Title: J. Virol. (in press)
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum targeting of the Red clover necrotic mosaic virus movement protein is associated with the replication of viral RNA1 but not that of RNA2. (2009)
  • Author(s): Kaido, M., Tsuno, Y., Mise, K.,, Okuno, T.
  • Journal Title: Virology 395(2) Pages: 232-242
  • Peer Reviewed
• [Journal Article] Melandrium yellow fleck bromovirus infects Arabidopsis thaliana and has genomic RNA sequence characteristics that are unique among bromoviruses. (2009)
  • Author(s): Narabayashi, T., Iwahashi, F., Kaido, M., Okuno, T.,, Mise, K.
  • Journal Title: Arch. Virol. 154(9) Pages: 1381-1389
  • Peer Reviewed
• [Journal Article] Host-dependent roles of the viral 5' untranslated region (UTR) in RNA stabilization and cap-independent translational enhancement mediated by the 3' UTR of Red clover necrotic mosaic virus RNA1. (2009)
  • Author(s): Sarawaneeyaruk, S., Iwakawa, H.-O., Mizumoto, H., Murakami, H., Kaido, M., Mise, K.,, Okuno, T.
  • Journal Title: Virology 391(1) Pages: 107-118
  • Peer Reviewed
• [Journal Article] Genetic analysis of a host determination mechanism of bromoviruses in Arabidopsis thaliana. (2009)
  • Author(s): Fujisaki, K., Iwahashi, F., Kaido, M., Okuno, T.,, Mise, K.
  • Journal Title: Virus Res. 140(1-2) Pages: 103-111
  • Peer Reviewed
• [Journal Article] First report of Bean common mosaic virus in yam bean (Pachyrhizus erosus (L. ) Urban) in Indonesia. (2008)
  • Author(s): Damayanti, T.A., Susilo, D., Nurlaelah, S., Sartiami, D., Okuno, T.,, Mise, K.
  • Journal Title: J. Gen. Plant Pathol. 74(6) Pages: 438-442
  • Peer Reviewed
• [Journal Article] A viral non-coding RNA generated by cis-element-mediated protection against 5'->3' RNA decay represses both cap-independent and cap-dependent translation. (2008)
  • Author(s): Iwakawa, H.-O., Mizumoto, H., Nagano, H., Imoto, Y., Takigawa, K., Sarawaneeyaruk, S., Kaido, M., Mise, K.,, Okuno, T.
  • Journal Title: J. Virol. 82(24) Pages: 10162-10174
  • Peer Reviewed
• [Journal Article] cis-Preferential requirement of a-1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1. (2008)
  • Author(s): Okamoto, K., Nagano, H., Iwakawa, H.-O., Mizumoto, H., Takeda, A., Kaido, M., Mise, K.,, Okuno, T.
  • Journal Title: Virology 375(1) Pages: 205-212
• [Journal Article] cis-Acting core RNA elements required for negative-strand RNA synthesis and cap-independent translation are separated in the 3'-untranslated region of Red clover necrotic mosaic virus RNA1. (2007)
  • Author(s): Iwakawa, H.-O., Kaido, M., Mise, K.,, Okuno, T.
  • Journal Title: Virology 369(1) Pages: 168-181
  • Peer Reviewed
• [Journal Article] Phosphorylation, interaction of the movement, coat proteins of Brome mosaic virus in infected barley protoplasts. (2007)
  • Author(s): Akamatsu, N., Takeda, A., Kishimoto, M., Kaido, M., Okuno, T., Mise, K.
  • Journal Title: Arch. Virol. 152(11) Pages: 2087-2093
  • Peer Reviewed
• [Journal Article] Downregulation of the NbNACa1 gene encoding a movement-protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana. Mol. (2007)
  • Author(s): Kaido, M., Inoue, Y., Takeda, Y., Sugiyama, K., Takeda, A., Mori, M., Tamai, A., Meshi, T., Okuno, T., Mise, K.
  • Journal Title: Plant-Microbe Interact 20(6) Pages: 671-681
• [Presentation] Melandrium yellow fleck bromovirus infects Arabidopsis thaliana and has unique characteristics in genomic RNA sequences among bromoviruses. (2009)
  • Author(s): Narabayashi, T., et al.
  • Organizer: 28th Annual Meeting of American Society for Virology.
  • Place of Presentation: Vancouver, BC, Canada
  • Year and Date: 2009-07-13
• [Presentation] Melandrium yellow fleck virus (MYFV) RNA3の5'非翻訳領域に存在する短いopen reading frame (ORF)がウイルス感染に与える影響. (2009)
  • Author(s): 楢林, ら
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市, 山形大学小白川キャンパス
  • Year and Date: 2009-03-27
• [Presentation] Melandrium yellow fleck virus (MYFV) RNA3の5'非翻訳領域の反復配列はNicotiana benthamianaでの全身感染に必須ではないがウイルスの感染競合に影響する. (2008)
  • Author(s): 楢林, ら
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江市, 国びきメッセ
  • Year and Date: 2008-04-27
• [Presentation] Spring beauty latent virus (SBLV)感染シロイヌナズナに誘導される全身壊疽病徴発現への病害抵抗性経路の関与. (2008)
  • Author(s): 岩橋, ら
  • Organizer: 平成20年度日本植物病理学会大会
  • Place of Presentation: 松江市, 国びきメッセ
  • Year and Date: 2008-04-27
• [Presentation] Two TIR-NB-LRR genes, SSB1A and SSB1B, interdependently function to develop severe symptoms upon infection with Spring beauty latent virus in Arabidopsis thaliana. Keystone Symposia on Molecular and Cellular Biology. (2008)
  • Author(s): Iwahashi, F., et al
  • Organizer: Plant Innate Immunity (Organized by Jonathan D.G. Jones and Jane Glazebrook)
  • Place of Presentation: Keystone Resort, Keystone, Colorado, USA
  • Year and Date: 2008-02-12
• [Presentation] ブロムモザイクウイルスの移行タンパク質の機能解析-リン酸化と管状構造-. (2007)
  • Author(s): 赤松, ら
  • Organizer: 第30回日本分子生物学会年会
  • Place of Presentation: 横浜市, パシフィコ横浜
  • Year and Date: 20071211-20071215
• [Presentation] Spring beauty latent virus (SBLV)感染シロイヌナズナに誘導される全身えそ病徴には2種のTIR-NB-LRR遺伝子が関与する. (2007)
  • Author(s): 猿渡, ら
  • Organizer: 平成19年度日本植物病理学会関西部会
  • Place of Presentation: 岐阜市, 岐阜大学
  • Year and Date: 2007-10-06
• [Presentation] Brome mosaic virus (BMV)の3a移行タンパク質のリン酸化と管状構造. (2007)
  • Author(s): 赤松, ら
  • Organizer: 平成19年度日本植物病理学会関西部会
  • Place of Presentation: 岐阜市, 岐阜大学
  • Year and Date: 2007-10-06
• [Book] ブロモウイルス感染シロイヌナズナにおける全身えそ病徴発現機構. 植物ウイルス病研究会レポート(大木理・鈴木信弘編) (2008)
  • Author(s): 三瀬和之、岩橋福松、猿渡洋介、林瑞恵、楢林大樹、乾裕江、藤崎恒喜、海道真典、奥野哲郎
  • Total Pages: 47-56
  • Publisher: 日本植物病理学会
• [Book] 病原性をもつ微生物(青木健次編) (2007)
  • Author(s): 三瀬和之
  • Total Pages: 44-50
  • Publisher: 微生物学
• [Book] ウイルス.微生物学(青木健次編) (2007)
  • Author(s): 三瀬和之
  • Total Pages: 39-41
  • Publisher: 化学同人
• [Book] ブロムモザイクウイルスの複製・移行と宿主因子. 微生物の病原性と植物の防御応答(上田一郎編) (2007)
  • Author(s): 三瀬和之
  • Total Pages: 173-180
  • Publisher: 北海道大学出版会
• [Remarks]
  • URL: http://www.plant-pathology.kais.kyoto-u.ac.jp/