2010 Fiscal Year Final Research Report
Interaction between Wolbachia exhibiting cytoplasmic incompatibility and its hosts
| Project/Area Number |
19380038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
NODA Hiroaki National Institute of Agrobiological Sciences, 昆虫・微生物間相互作用研究ユニット, ユニット長 (40343991)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 昆虫 / 共生微生物 / ウォルバキア / 細胞質不和合性 / マイクロアレイ |
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| Research Abstract |
Various aspects of intracellular symbionts, Wolbachia and Cardinium, were studied, with special reference to distribution among arthropods, cultivation in insect cell lines, gene expression profile of host cells, and classification of Cardinium. Cytoplasmic incompatibility was caused independently by Wolbachia and Cardinium in white-backed planthopper, Sogatella furcifera. The bacteria of the planthopper were successfully cultivated in insect cell lines. Immune response of host cells against bacteria was examined using microarrays. Caridinium induced immune response in the host cells whereas Wolbachia did not. Infection of Cardinium was surveyed in planthoppers, spider mites, and biting midges, which resulted in reclassification of the order Cardinium.
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