Analysis of novel cell surface proteins affecting cell shape of bacteria

2009 Fiscal Year Self-evaluation Report

• Project/Area Number: 19380047
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Shinshu University
• Principal Investigator: SEKIGUCHI Junichi Shinshu University, 総合工学系研究科, 教授 (80111053)
• Project Period (FY): 2007 – 2009
• Keywords: 微生物機能 / 表層蛋白質 / 細胞形態 / 細胞壁溶解酵素 / インヒビター / 枯草菌

Research Abstract

細胞の形を作る因子の研究は,細胞増殖・分裂,細胞壁の合成,分解にも直接関係する重要な領域である.そこで細胞の形に影響することが解った機能未知遺伝子とその産物の機能を,分子生物学的に解明し,細胞の形を作るメカニズムを明らかにする.

Research Products

• [Journal Article] 枯草菌における細胞の分離 (2009)
  • Author(s): 橋本昌征, 山本博規, 関口順一
  • Journal Title: 繊維と工業 65(8) Pages: 282-286
• [Journal Article] Identification and characterization of new cell wall hydrolase, CwlT : a two-domain hydrolase of N-acetylmuramidase and D, L-endopeptidase (2008)
  • Author(s): Fukushima, T., T. Kitajima, H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, J. Sekiguchi
  • Journal Title: J.Biol.Chem. 283(17) Pages: 11117-11125
  • Peer Reviewed
• [Journal Article] Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis (2008)
  • Author(s): Yamamoto, H., M. Hashimoto, Y. Higashitsuji, H. Harada, N. Hariyama, L. Takahashi, T. Iwashita, S. Ooiwa, J. Sekiguchi
  • Journal Title: Mol.Microbiol. 70(1) Pages: 168-182
  • Peer Reviewed
• [Journal Article] The major and minor wall teichoic acids prevent the sidewall localization of vegetative D, L-endopeptidase LytF in Bacillus subtilis (2008)
  • Author(s): Yamamoto, H., Y. Miyake, M. Hisaoka, S. Kurosawa, J. Sekiguchi
  • Journal Title: Mol.Microbiol. 70(2) Pages: 297-310
  • Peer Reviewed
• [Presentation] Bacillus subtilis CwlP in the SP-beta prophage comprises two novel cell wall hydrolase domains (2009)
  • Author(s): I Putu Sudiarta, T. Fukushima, J. Sekiguchi
  • Organizer: BioMicroWorld 2009
  • Place of Presentation: リスボン大学 (リスボン)
  • Year and Date: 2009-12-02
• [Presentation] ゲノムで紐解く細胞壁結合蛋白質群の機能 (2009)
  • Author(s): 関口順一, 福島達也, 橋本昌征, 山本博規
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 信州大学 (松本市)
  • Year and Date: 2009-09-16
• [Presentation] PdaC (YjeA) deacetylates the acetyl groups of N-acetylglucosamine in the chitin oligomers and N-acetylmuramic acid in peptidoglycan-Biochemical approach for identification of PdaC in Bacillus subtilis- (2009)
  • Author(s): K. Kobayashi, T. Kodama, T. Fukushima, K. Ara, K. Ozaki, J. Sekiguchi
  • Organizer: 5^<th> Internatl.Conf.on Gram-Positive Microorganisms
  • Place of Presentation: Catamaran ホテル (サンディエゴ)
  • Year and Date: 2009-06-15
• [Patent(Industrial Property Rights)] p60エンドペプチダーゼ阻害剤 (2008)
  • Inventor(s): 荒勝俊, 阪本知生, 山本博規, 針山望, 関口順一
  • Industrial Property Rights Holder: 花王、信州大学
  • Industrial Property Number: 特許特願2008-78828、特開2009-227648
  • Filing Date: 2008-03-25
• [Patent(Industrial Property Rights)] 溶菌酵素阻害剤、溶菌抑制剤及びポリーガンマーグルタミン酸の分解抑制剤及びポリーガンマーグルタミン酸の製造方法 (2007)
  • Inventor(s): 荒勝俊, 関口順一, 山本博規, 原田宏之
  • Industrial Property Rights Holder: 花王、信州大学
  • Industrial Property Number: 特許特願2007-268523、特開2008-118985【NI0700043】
  • Filing Date: 2007-10-16