2010 Fiscal Year Final Research Report
Exploration of target proteins for ubiquitination using fluorescence anisotropy measurement
| Project/Area Number |
19380056
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Aoyama Gakuin University (2010)
Japan Agency for Marine-Earth Science and Technology (2007-2009) |
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Principal Investigator |
ABE Fumiyoshi Aoyama Gakuin University, 理工学部, 准教授 (30360746)
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| Project Period (FY) |
2007 – 2010
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| Keywords | 蛍光偏光解消法 / 時間分解測定 / 細胞膜物性 / 蛍光寿命 / 出芽酵母 / ユビキチン機構 / 回転ブラウン運動 / 分子間相互作用 |
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| Research Abstract |
This study approaches the finding of novel factors that interact with ubiquitination-related proteins by monitoring the rotational motion of proteins. First, I established the experimental procedure to quantify the rotational motion of membrane lipids in yeast. The defect in ergosterol biosynthesis resulted in a decrease in membrane rigidity and an increase in the rotational lipid motion. The measurement of fluorescein-labeled model proteins suggests that this technique is valid for proteins of less than 10 kDa. Sna3 was identified as a factor that potentially interacted with Rsp5 ubiquitin ligase. In the future study, I investigate the molecular interaction between Rsp5 and Sna3 using the system employed in this study.
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[Journal Article] Sorting defects of the tryptophan permease Tat2 in an erg2 yeast mutant2009
Author(s)
Daicho, K., Makino, N. Hiraki, T., Ueno, M., Uritani, M., Abe, F., Ushimaru, T.
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Journal Title
FEMS Microbiol. Lett 298
Pages: 218-227
Peer Reviewed
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[Journal Article] Control of Protein and Sterol Trafficking by Antagonistic Activities of a P4-ATPase and Oxysterol Binding Protein Homologue2009
Author(s)
Muthusamy, B-P, Raychaudhuri, S., Natarajan, P., Abe, F., Liu, K., Prinz, W. A. Graham, T. R.
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Journal Title
Mol. Biol. Cell 20
Pages: 2920-2931
Peer Reviewed
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[Journal Article] Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology2009
Author(s)
Guan, X., Souza, C., Pichler, H., Dewhurst, G., Schaad, O., Kajiwara, K., Wakabayashi, H., Ivanova, T., Castillon, G., Piccolis, M., Abe, F., Loewith, R., Funato, K., Wenk, M., Riezman, H.
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Journal Title
Mol. Biol. Cell 20
Pages: 2083-2095
Peer Reviewed
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[Journal Article] Identification and characterization of two alternative σ factors of RNA polymerase in the deep-sea piezophilic bacterium, Shewanella violacea strain DSS122009
Author(s)
Kawano, H., Takahashi, H., Abe, F., Kato, C., Horikoshi, K.
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Journal Title
Biochem. Biosci. Biotechnol. 73
Pages: 200-202
Peer Reviewed
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[Journal Article] Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase2008
Author(s)
Ohmae, E., Tatsuta, M., Abe, F., Kato, C., Tanaka, N., Kunugi, S., Gekko, K.
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Journal Title
Biochim. Biophys. Acta 1784
Pages: 1115-1121
Peer Reviewed
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