2009 Fiscal Year Final Research Report
Study on the mechanism of the ribonucleoprotein complex
| Project/Area Number |
19380060
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
KIMURA Makoto Kyushu University, 大学院・農学研究院, 教授 (10204992)
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| Co-Investigator(Kenkyū-buntansha) |
KAKUTA Yoshimitsu 九州大学, 大学院・農学研究院, 准教授 (00314360)
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| Project Period (FY) |
2007 – 2009
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| Research Abstract |
Ribonuclease P (RNase P) is composed of a catalytic RNA (PhopRNA) and five proteins (PhoPop5, PhoRpp21, PhoRpp29, PhoRpp30, PhoRpp38). In this study, we determined the crystal structure of the protein complex composed of PhoRpp21 and PhoRpp29. Furthermore, we examined the protein binding sites on PhopRNA by biochemical methods and analyzed the activated structure of RNase P RNA (PhopRNA) in Pyrococcus horikoshii OT3 was characterized by circular dichroism (CD) and ultraviolet (UV) absorbance spectra.
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