The new method to identify small molecules binding proteins by usingmutants and genome sequences

2009 Fiscal Year Final Research Report

• Project/Area Number: 19380066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tokyo University of Science
• Principal Investigator: 菅原 二三男 Tokyo University of Science, 理工学部, 教授 (30192123)
• Project Period (FY): 2007 – 2009
• Keywords: 受容体 / 結合タンパク質 / 遺伝子変異体 / ゲノム / ファージディスプレイ / QCM / 結合アミノ酸配列

Research Abstract

The present study aimed at the identification of the action new and in vivo target protein of the establishment of the new medicine receptivity evaluation method that used gene mutation Drosophila melanogaster and CPT that used it.
CPT-20-B connecting peptide (NSASRGGSQRGRGEH) is identified by using the T7 phage display method with crystal departure pendulum microbalance (QCM) device. This peptide sequencing showed each array and the homology of human HshnRNP A1 and Drosophila melanogaster DmHrb87F. CPT united from the bead pull-down examination that used HeLa cell meltable picture with HshnRNP A1. CPT made HshnRNP A1-GST united changing protein, did the QCM interaction analysis, and was HshnRNP A1 and was dissociation constant 82.7 nM.
As a result of the microbial sensitivity test, by gene loss Drosophila melanogaster, two Hrb87FKG02089 and Hrb87FBG02743 that was the DmHrb87F gene mutant showed CPT high receptivity compared with the wild strain. Topo I has working that cancels a super-coil of plasmid DNA. This topology change was observed under CPT and the HshnRNP A1 existence. CPT obstructed the topo I revitalization as reported. Moreover, hnRNP A1 also similarly obstructed the topo I revitalization. In addition, CPT has improved the topo I active inhibition to HshnRNP A1 under both existence.
HshnRNP A1 or DmHrb87F united from the above-mentioned result with CPT, and the possibility of adjusting the topo I revitalization was suggested.

Research Products

• [Journal Article] A sulfoglycolipid beta-sulfoquinovosyldiacylglycerol (betaSQDG) binds to Met1-Arg95 region of murine DNA polymerase lambda (Mmpol lambda) and inhibits its nuclear transit. (2010)
  • Author(s): Takakusagi K, Takakusagi Y, Ohta K, Aoki S, Sugawara F, Sakaguchi K.
  • Journal Title: Protein Eng Des Sel 23(2) Pages: 51-60
• [Journal Article] 3-O-methylfunicone, a selective inhibitor of mammalian Y-family DNA polymerases from an Australian sea salt fungal strain. (2009)
  • Author(s): Mizushina Y, Motoshima H, Yamaguchi Y, Takeuchi T, Hirano K, Sugawara F, Yoshida H.
  • Journal Title: Mar Drugs 7(4) Pages: 624-39
• [Journal Article] Antitumor antibiotic fostriecin covalently binds to cysteine-269 residue of protein phosphatase 2A catalytic subunit in mammalian cells. (2009)
  • Author(s): Takeuchi T, Takahashi N, Ishi K, Kusayanagi T, Kuramochi K, Sugawara F.
  • Journal Title: Bioorg Med Chem 17(23),17(23) Pages: 8113-22. 1, 8113-22
• [Journal Article] Synthesis and structure-activity relationships of dehydroaltenusin derivatives as selective DNA polymerase alpha inhibitors. (2009)
  • Author(s): Kuramochi K, Fukudome K, Kuriyama I, Takeuchi T, Sato Y, Kamisuki S, Tsubaki K, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Bioorg Med Chem 17(20) Pages: 7227-38
• [Journal Article] Structure and activity relationship of monogalactosyl diacylglycerols, which selectively inhibited in vitro mammalian replicative DNA polymerase activity and human cancer cell growth. (2009)
  • Author(s): Matsui Y, Hada T, Maeda N, Sato Y, Yamaguchi Y, Takeuchi T, Takemura M, Sugawara F, Sakaguchi K, Yoshida H, Mizushina Y.
  • Journal Title: Cancer Lett 283(1) Pages: 101-7
• [Journal Article] Penicilliols A and B, novel inhibitors specific to mammalian Y-family DNA polymerases. (2009)
  • Author(s): Kimura T, Takeuchi T, Kumamoto-Yonezawa Y, Ohashi E, Ohmori H, Masutani C, Hanaoka F, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Bioorg Med Chem 17(5) Pages: 1811-6
• [Journal Article] Chlorogenic acid facilitates root hair formation in lettuce seedlings. (2009)
  • Author(s): Narukawa M, Kanbara K, Tominaga Y, Aitani Y, Fukuda K, Kodama T, Murayama N, Nara Y, Arai T, Konno M, Kamisuki S, Sugawara F, Iwai M, Inoue Y.
  • Journal Title: Plant Cell Physiol 50(3) Pages: 504-14
• [Journal Article] Identification of trimannoside-recognizing peptide sequences from a T7 phage display screen using a QCM device. (2009)
  • Author(s): Nishiyama K, Takakusagi Y, Kusayanagi T, Matsumoto Y, Habu S, Kuramochi K, Sugawara F, Sakaguchi K, Takahashi H, Natsugari H, Kobayashi S.
  • Journal Title: Bioorg Med Chem 17(1) Pages: 195-202
• [Journal Article] The inhibitory action of kohamaic acid A derivatives on mammalian DNA polymerase beta. (2008)
  • Author(s): Mizushina Y, Manita D, Takeuchi T, Sugawara F, Kumamoto-Yonezawa Y, Matsui Y, Takemura M, Sasaki M, Yoshida H, Takikawa H.
  • Journal Title: Molecules 14(1) Pages: 102-21
• [Journal Article] Effect of dehydroaltenusin-C12 derivative, a selective DNA polymerase alpha inhibitor, on DNA replication in cultured cells. (2008)
  • Author(s): Kuriyama I, Mizuno T, Fukudome K, Kuramochi K, Tsubaki K, Usui T, Imamoto N, Sakaguchi K, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Molecules 13(12) Pages: 2948-61
• [Journal Article] Synthesis and biological activities of neoechinulin A derivatives: new aspects of structure-activity relationships for neoechinulin A. (2008)
  • Author(s): Kuramochi K, Ohnishi K, Fujieda S, Nakajima M, Saitoh Y, Watanabe N, Takeuchi T, Nakazaki A, Sugawara F, Arai T, Kobayashi S.
  • Journal Title: Chem Pharm Bull 56(12) Pages: 1738-43
• [Journal Article] Identification of Small Molecule Binding Molecules by Affinity Purification Using a Specific Ligand Immobilized on PEGA Resin. (2008)
  • Author(s): Kuramochi K, Miyano Y, Enomoto Y, Takeuchi R, Ishi K, Takakusagi Y, Saitoh T, Fukudome K, Manita D, Takeda Y, Kobayashi S, Sakaguchi K, Sugawara F.
  • Journal Title: Bioconjug Chem 19(12) Pages: 2417-26
• [Journal Article] Synthesis of Neoechinulin A and Derivatives. (2008)
  • Author(s): Kuranochi K, Aoki T, Nakazaki A, Kamisuki S, Takeno M, Ohnishi K, Kimoto K, Watanabe N, Kamakura T, Arai T, Sugawara F, Kobayashi K.
  • Journal Title: Synthesis 23 Pages: 3810-18
• [Journal Article] The specific inhibitory effect of demethoxydehydroaltenusin, a derivative of dehydroaltenusin, on mammalian DNA polymerase alpha. (2008)
  • Author(s): Kuriyama I, Fukudome K, Kamisuki S, Kuramochi K, Tsubaki K, Sakaguchi K, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Int J Mol Med 22(6) Pages: 793-9
• [Journal Article] Efficient one-cycle affinity selection of binding proteins or peptides specific for a small-molecule using a T7 phage display pool. (2008)
  • Author(s): Takakusagi Y, Kuramochi K, Takagi M, Kusayanagi T, Manita D, Ozawa H, Iwakiri K, Takakusagi K, Miyano Y, Nakazaki A, Kobayashi S, Sugawara F, Sakaguchi K.
  • Journal Title: Bioorg Med Chem 16(22) Pages: 9837-46
• [Journal Article] Neoechinulin A protects PC12 cells against MPP+-induced cytotoxicity. (2008)
  • Author(s): Kajimura Y, Aoki T, Kuramochi K, Kobayashi S, Sugawara F, Watanabe N, Arai T.
  • Journal Title: J Antibiot (Tokyo) 61(5) Pages: 330-3
• [Journal Article] Identification of a methotrexate-binding peptide from a T7 phage display screen using a QCM device. (2008)
  • Author(s): Takakusagi Y, Kuroiwa Y, Sugawara F, Sakaguchi K.
  • Journal Title: Bioorg Med Chem 52(3) Pages: 556-62
• [Journal Article] Identification of macrolide antibiotic-binding Human_p8 protein. (2008)
  • Author(s): Morimura T, Hashiba M, Kameda H, Takami M, Takahama H, Ohshige M, Sugawara F.
  • Journal Title: J Antibiot (Tokyo) 61(5) Pages: 291-6
• [Journal Article] A facile method to screen inhibitors of protein-protein interactions including MDM2-p53 displayed on T7 phage. (2008)
  • Author(s): Ishi K, Sugawara F.
  • Journal Title: Biochem Pharmacol 75(9) Pages: 1743-50
• [Journal Article] Downregulation of Tie2 gene by a novel antitumor sulfolipid, 3'-sulfoquinovosyl-1'-monoacylglycerol, targeting angiogenesis. (2008)
  • Author(s): Mori Y, Sahara H, Matsumoto K, Takahashi N, Yamazaki T, Ohta K, Aoki S, Miura M, Sugawara F, Sakaguchi K, Sato N.
  • Journal Title: Cancer Sci 99(5) Pages: 1063-70
• [Journal Article] Syntheses and applications of fluorescent and biotinylated epolactaene derivatives: Epolactaene and its derivative induce disulfide formation. (2008)
  • Author(s): Kuramochi K, Yukizawa S, Ikeda S, Sunoki T, Arai S, Matsui R, Morita A, Mizushina Y, Sakaguchi K, Sugawara F, Ikekita M, Kobayashi S.
  • Journal Title: Bioorg Med Chem 16(9) Pages: 5039-49
• [Journal Article] Hymenoic acid, a novel specific inhibitor of human DNA polymerase lambda from a fungus of Hymenochaetaceae sp. (2008)
  • Author(s): Nishida M, Ida N, Horio M, Takeuchi T, Kamisuki S, Murata H, Kuramochi K, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Bioorg Med Chem 16(9) Pages: 5115-22
• [Journal Article] The inhibitory action of SQDG (sulfoquinovosyl diacylglycerol) from spinach on Cdt1-geminin interaction. (2008)
  • Author(s): Mizushina Y, Takeuchi T, Hada T, Maeda N, Sugawara F, Yoshida H, Fujita M.
  • Journal Title: Biochimie 90(6) Pages: 947-56
• [Journal Article] Novel azaphilones, kasanosins A and B, which are specific inhibitors of eukaryotic DNA polymerases beta and lambda from Talaromyces sp. (2008)
  • Author(s): Kimura T, Nishida M, Kuramochi K, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Bioorg Med Chem 16(8) Pages: 4594-9
• [Journal Article] Inhibitory action of polyunsaturated fatty acids on Cdt1-geminin interaction. (2008)
  • Author(s): Mizushina Y, Takeuchi T, Takakusagi Y, Sugawara F, Sakaguchi K, Yoshida H, Fujita M.
  • Journal Title: Int J Mol Med 21(3) Pages: 281-9
• [Journal Article] 1-Deoxyrubralactone, a novel specific inhibitor of families X and Y of eukaryotic DNA polymerases from a fungal strain derived from sea algae. (2008)
  • Author(s): Naganuma M, Nishida M, Kuramochi K, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Bioorg Med Chem 16(6) Pages: 2939-44
• [Journal Article] Coenzyme Q(10) as a potent compound that inhibits Cdt1-geminin interaction. (2008)
  • Author(s): Mizushina Y, Takeuchi T, Takakusagi Y, Yonezawa Y, Mizuno T, Yanagi K, Imamoto N, Sugawara F, Sakaguchi K, Yoshida H, Fujita M.
  • Journal Title: Biochim Biophys Acta 1780(2) Pages: 203-13
• [Journal Article] Chemical properties of fatty acid derivatives as inhibitors of DNA polymerases. (2007)
  • Author(s): Nakamura R, Takeuchi R, Kuramochi K, Mizushina Y, Ishimaru C, Takakusagi Y, Takemura M, Kobayashi S, Yoshida H, Sugawara F, Sakaguchi K.
  • Journal Title: Org Biomol Chem 5(24) Pages: 3912-21
• [Journal Article] Screening of paclitaxel-binding molecules from a library of random peptides displayed on T7 phage particles using paclitaxel-photoimmobilized resin. (2007)
  • Author(s): Aoki S, Morohashi K, Sunoki T, Kuramochi K, Kobayashi S, Sugawara F.
  • Journal Title: Bioconjug Chem 18(6) Pages: 1981-6
• [Journal Article] Structure-activity Relationships of Neoechinulin A Analogues with Cytoprotection against Peroxynitrite-induced PC12 Cell Death. (2007)
  • Author(s): Kimoto K, Aoki T, Shibata Y, Kamisuki S, Sugawara F, Kuramochi K, Nakazaki A, Kobayashi S, Kuroiwa K, Watanabe N, Arai T.
  • Journal Title: J Antibiot (Tokyo) 60(10) Pages: 614-21
• [Journal Article] Identification of C10 biotinylated camptothecin (CPT-10-B) binding peptides using T7 phage display screen on a QCM device. (2007)
  • Author(s): Takakusagi Y, Takakusagi K, Kuramochi K, Kobayashi S, Sugawara F, Sakaguchi K.
  • Journal Title: Bioorg Med Chem 15(24) Pages: 7590-8
• [Journal Article] An approach to evaluate two-electron reduction of 9, 10-phenanthraquinone and redox activity of the hydroquinone associated with oxidative stress. (2007)
  • Author(s): Taguchi K, Fujii S, Yamano S, Cho AK, Kamisuki S, Nakai Y, Sugawara F, Froines JR, Kumagai Y.
  • Journal Title: Free Radic Biol Med 43(5) Pages: 789-99
• [Journal Article] Effects of a new immunosuppressive agent, beta-SQAG9, in swine kidney transplantation. (2007)
  • Author(s): Tanaka T, Kitamura H, Sahara H, Imai A, Itoh Y, Honma I, Sato E, Kobayashi K, Maeda T, Takenouchi M, Ohta K, Sugawara F, Sakaguchi K, Ando A, Inoko H, Sato N, Tsukamoto T.
  • Journal Title: Transpl Immunol 18(1) Pages: 67-71
• [Journal Article] Inhibitory action of polyunsaturated fatty acids on IMP dehydrogenase. (2007)
  • Author(s): Mizushina Y, Dairaku I, Yanaka N, Takeuchi T, Ishimaru C, Sugawara F, Yoshida H, Kato N.
  • Journal Title: Biochimie 89(5) Pages: 581-90
• [Journal Article] Nodulisporol and Nodulisporone, novel specific inhibitors of human DNA polymerase lambda from a fungus, Nodulisporium sp. (2007)
  • Author(s): Kamisuki S, Ishimaru C, Onoda K, Kuriyama I, Ida N, Sugawara F, Yoshida H, Mizushina Y.
  • Journal Title: Bioorg Med Chem 15(9) Pages: 3109-14
• [Journal Article] Sulfoglycolipids as candidate antiangiogenic radiosensitizers (2007)
  • Author(s): Miura M, Sakimoto I, Ohta K, Sugawara F, Sakaguchi K.
  • Journal Title: Anticancer Drugs 18(1) Pages: 1-5
• [Journal Article] Structure-activity relationship of a glycolipid, sulfoquinovosyl diacylglycerol, with the DNA binding activity of p53. (2007)
  • Author(s): Iijima H, Kasai N, Chiku H, Takeuchi T, Kuramochi K, Hanashima S, Kobayashi S, Sugawara F, Sakaguchi K, Yoshida H, Mizushina Y.
  • Journal Title: Int J Mol Med 19(1) Pages: 41-8
• [Journal Article] Anti-tumor effects of dehydroaltenusin, a specific inhibitor of mammalian DNA polymerase alpha. (2007)
  • Author(s): Maeda N, Kokai Y, Ohtani S, Sahara H, Kuriyama I, Kamisuki S, Takahashi S, Sakaguchi K, Sugawara F, Yoshida H, Sato N, Mizushina Y.
  • Journal Title: Biochem Biophys Res Commun 352(2) Pages: 390-6
• [Journal Article] Inhibitory effect of sulfoquinovosyl diacylglycerol on prokaryotic DNA polymerase I activity and cell growth of Escherichia coli. (2006)
  • Author(s): Furukawa T, Nishida M, Hada T, Kuramochi K, Sugawara F, Kobayashi S, Iijima H, Shimada H, Yoshida H, Mizushina Y.
  • Journal Title: J Oleo Sci 56(1) Pages: 43-7
• [Journal Article] Podophyllotoxin directly binds a hinge domain in E2 of HPV and inhibits an E2/E7 interaction in vitro.
  • Author(s): Saitoh T, Kuramochi K, Imai T, Takata KI, Takehara M, Kobayashi S, Sakaguchi K, Sugawara F.
  • Journal Title: Bioorg Med Chem 16(10) Pages: 5815-25
• [Presentation] 線虫ガレクチンLEC-6による糖鎖認識機構解明のための分子プローブの合成及びその評価 (2010)
  • Author(s): 西山和沙, 山田篤, 草柳友恵, 松本勇記, 高草木洋一, 菅原二三男, 坂口謙吾, 小林進, 武内智春, 笠井献一, 夏苅英昭, 高橋秀依
  • Organizer: 日本薬学会第130年会
  • Year and Date: 2010-03-28
• [Presentation] エポラクタエン及び誘導体の化学と生物:全合成から作用機構解析 (2010)
  • Author(s): 倉持幸司, 椿一典, 水品善之, 菅原二三男, 池北雅彦, 小林進
  • Organizer: 第4回連携大学院セミナー京都「先端バイオイメージングと基盤技術」
  • Year and Date: 2010-01-18
• [Presentation] YファミリーDNAポリメラーゼ選択的阻害剤:Penicilliol A, Penicilliol B (2009)
  • Author(s): 水品善之, 木村拓馬, 竹内倫文, 米澤裕子, 大橋英治, 大森治夫, 益谷央豪, 花岡文雄, 吉田弘美, 菅原二三男, 坂口謙吾
  • Organizer: 第32回日本分子生物学会
  • Year and Date: 2009-12-09
• [Presentation] T7 PD法による低分子化合物とタンパク質の相互作用の検出: QCM装置とRELICサーバーの利用がもたらす実用性と可能性 (2009)
  • Author(s): 高草木洋一, 高草木香織, 菅原二三男, 坂口謙吾
  • Organizer: 第32回日本分子生物学会
  • Year and Date: 2009-12-09
• [Presentation] 含硫糖脂質SQAGのhVEGF165結合領域の同定及び結合様式の解明 (2009)
  • Author(s): 高草木香織, 高草木洋一, 松本勇記, 鷹見美穂子, 井田紀子, 青木仁子, 村田寛, 太田慶祐, 菅原二三男, 坂口謙吾
  • Organizer: 第32回日本分子生物学会
  • Year and Date: 2009-12-09
• [Presentation] T7ファージディスプレイ法を用いて同定したアンジオモチンとロキシスロマイシンの相互作用の検証 (2009)
  • Author(s): 鈴木愛こ, 高草木洋一, 渡辺まどか, 松本勇記, 戸井崎絢, 鈴木孝洋, 菅原二三男, 小林進, 坂口謙吾
  • Organizer: 第32回日本分子生物学会
  • Year and Date: 2009-12-09
• [Presentation] エポラクタエン及び誘導体のチオール反応性 (2009)
  • Author(s): 倉持幸司, 椿一典, 水品善之, 菅原二三男, 池北雅彦, 小林進
  • Organizer: 日本農芸化学会関西支部例会
  • Year and Date: 2009-12-05
• [Presentation] カバノアナタケから同定された新規化合物DDTCTの細胞増殖抑制効果 (2009)
  • Author(s): 望月万里, 森田明典, 羽染芳宗, 井田紀子, 村田寛, 船津修, 菅原二三男, 池北雅彦
  • Organizer: 第82回日本生化学会大会
  • Year and Date: 2009-10-22
• [Presentation] X-family DNAポリメラーゼによる生物多様性の調節 (2009)
  • Author(s): 小寺啓文, 武内亮, 半澤直人, 内山幸伸, 美和秀胤, 菅原二三男, 坂口謙吾
  • Organizer: 第11回日本進化学会大会
  • Year and Date: 2009-09-02
• [Presentation] Self-assembled monolayer (SAM) of small organic molecule for efficient random-peptide phage display selection using a cuvette type quartz-crystal microbalance (2009)
  • Author(s): Takakusagi Y, Suzuki A, Sugawara F, Kobayashi S, Sakaguchi K
  • Organizer: (QCM) device ICCE-17
  • Year and Date: 2009-07-26
• [Presentation] Camptothecins : Inhibition of the E-prostanoid receptor subtype 1 (2009)
  • Author(s): Takakusagi Y, Manita D, Okado M, Nakayama M, Takakusagi K, Sugawara F, Sakaguchi K
  • Organizer: (EP1) signaling pathways and its' mechanistic aspects The 50th Anniversary Meeting of the American Society of Pharmacognosy
  • Year and Date: 2009-06-27
• [Presentation] QCM-phage display: Identification of doxorubicin-binding protein (part III) (2009)
  • Author(s): Kusayanagi T, Takakusagi Y, Takeuchi T, Sakaguchi K, Sugawara F
  • Organizer: The 50th Anniversary Meeting of the American Society of Pharmacognosy
  • Year and Date: 2009-06-27
• [Presentation] 海洋天然物SQAGによるDNA polymerase lambdaの阻害様式の解明 (2009)
  • Author(s): 高草木香織, 太田慶祐, 高草木洋一, 青木仁子, 菅原二三男, 坂口謙吾
  • Organizer: 日本ケミカルバイオロジー学会第4回年会
  • Year and Date: 2009-05-18
• [Presentation] ニガウリ由来のmonoacylgalactosyl diacylglycerol (MGDG)のDNAポリメラーゼ阻害活性とヒト癌増殖抑制活性 (2009)
  • Author(s): 松井由樹, 羽田尚彦, 佐藤義大, 山口素弘, 竹内倫文, 坂口謙吾, 菅原二三男, 水品義之, 吉田弘美
  • Organizer: 日本農芸化学会2009年度(平成21年度)大会
  • Year and Date: 2009-03-27
• [Presentation] 浜辺に生息する苔に寄生する真菌(Penicillium deleae)ユリアの新規物質peniciliol AおよびBの生理活性 (2009)
  • Author(s): 水品義之, 木村拓馬, 竹内倫文, 菅原二三男
  • Organizer: 日本農芸化学会2009年度(平成21年度)大会
  • Year and Date: 2009-03-27
• [Presentation] T7ファージディスプレイ法を用いたタンパク質間相互作用阻害剤の探索法の改良 (2009)
  • Author(s): 齊藤雄己, 真仁田大輔, 菅原二三男
  • Organizer: 日本農芸化学会2009年度(平成21年度)大会
  • Year and Date: 2009-03-27
• [Presentation] クルクミン誘導体の抗炎症作用 (2009)
  • Author(s): 西田真之, 吉田優, 東健, 福留啓志, 清水恭平, 竹内倫文, 大澤俊彦, 菅原二三男, 水品義之, 吉田弘美
  • Organizer: 日本農芸化学会2009年度(平成21年度)大会
  • Year and Date: 2009-03-27
• [Presentation] トリマンノース由来の分子プローブの合成とQCM-PD-SAM法を用いた結合ペプチドの同定 (2009)
  • Author(s): 西山和沙, 高草木洋一, 草柳友恵, 松本勇記, 羽生紫織, 倉持幸司, 菅原二三男, 坂口謙吾, 高橋秀依, 夏苅英昭, 小林進
  • Organizer: 日本薬学会第129年会
  • Year and Date: 2009-03-26
• [Presentation] Antitumor Antibiotic Fostriecin Targets Cystein-269 of the Protein Phosphatase 2A Catalitic Subunit in Mammailan Cells with a Covalent Bond. (2009)
  • Author(s): Toshifumi Takeuchi, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-16
• [Presentation] Developement of Phage Display with Quartz Crystal Microbalance Method. (2009)
  • Author(s): Tomoe Kusayanagi, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-16
• [Presentation] A Facile Method to inhibit Protein-Protein Interactions with Phage Display Method. (2009)
  • Author(s): Takeki Saitoh, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-16
• [Presentation] Isolation of Natural Products (2) (2009)
  • Author(s): Yoshihiro Sato, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-16
• [Presentation] Synthesis of Natural Products (1): The Radio Sensitizer SQ series. (2009)
  • Author(s): Hiroshi Murata, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-16
• [Presentation] Sinthesis of Natural Products (2): Bioactive Compounds. (2009)
  • Author(s): Hiroaki Ishii, Kyohei Shimizu, Satomi Shimura, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-16
• [Presentation] New Screening Methods for Protein-Protein Interaction Inhibitors Based on FRET and Phage Display. (2009)
  • Author(s): Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-15
• [Presentation] Isolation of Natural Products (1) (2009)
  • Author(s): Takuma Kimura, Fumio Sugawara
  • Organizer: 3rd Tokyo Uiniversity of Science Interanational Collaboration Workshop
  • Year and Date: 2009-03-15
• [Presentation] QCM T7ファージディスプレイ法:新規ドキソルビシン結合タンパク質の探索 (2008)
  • Author(s): 草柳友恵, 倉持幸司, 高草木洋一, 菅原二三男
  • Organizer: (第二報)先端科学セミナー京都ケミカルバイオロジーシンポジウム
  • Year and Date: 2008-12-15
• [Presentation] 抗腫瘍活性物質カンプトテシンの新規作用標的タンパク質の探索 (2008)
  • Author(s): 真仁田大輔, 高草木洋一, 倉持幸司, 菅原二三男
  • Organizer: 先端科学セミナー京都ケミカルバイオロジーシンポジウム
  • Year and Date: 2008-12-15
• [Presentation] 固相合成用樹脂を利用した結合タンパク質探索 (2008)
  • Author(s): 榎本好宏, 倉持幸司, 宮野友香, 石一智, 高草木洋一, 斉藤雄己, 小林進, 坂口謙吾, 菅原二三男
  • Organizer: 先端科学セミナー京都ケミカルバイオロジーシンポジウム
  • Year and Date: 2008-12-15
• [Presentation] T7ファージディスプレイ法を用いたリダイフェンBの新規標的タンパク質の探索 (2008)
  • Author(s): 九十田千子, 高草木洋一, 今井孝彦, 椎名勇, 坂口謙吾, 池北雅彦, 菅原二三男
  • Organizer: 先端科学セミナー京都ケミカルバイオロジーシンポジウム
  • Year and Date: 2008-12-15
• [Presentation] カバノアナタケから同定されたDDTCTの細胞増殖抑制効果 (2008)
  • Author(s): 望月万里, 森田明典, 羽染芳宗, 井田紀子, 村田寛, 船津修, 菅原二三男, 池北雅彦
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Year and Date: 2008-12-10
• [Presentation] クルクミンとその誘導体による炎症抑制効果 (2008)
  • Author(s): 西田真之, 吉田優, 東健, 福留啓志, 竹内倫文, 倉持幸司, 菅原二三男, 坂口謙吾, 吉田弘美, 水品善之
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Year and Date: 2008-12-09
• [Presentation] T7 phage display法を用いたロキシスロマイシン結合タンパク質の探索 (2008)
  • Author(s): 鈴木愛こ, 高草木洋一, 渡邊まどか, 松本勇記, 菅原二三男, 坂口謙吾
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Year and Date: 2008-12-09
• [Presentation] T7 phage display法を用いたPFOS結合タンパク質の探索 (2008)
  • Author(s): 岡戸真実, 宮野友香, 崎元一平, 志村聡美, 草柳友恵, 武内亮, 高草木香織, 松本勇記, 中崎敦夫, 竹内倫文, 倉持幸司, 高草木洋一, 太田慶祐, 三浦雅彦, 小林進, 菅原二三男, 坂口謙吾
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Year and Date: 2008-12-09
• [Presentation] QCM T7ファージディスプレイ法:新規ドキソルビシン結合タンパク質の探索 (2008)
  • Author(s): 草柳友恵, 真仁田大輔, 岩切佳那子, 倉持幸司, 高草木洋一, 中崎敦夫, 小林進, 坂口謙吾, 菅原二三男
  • Organizer: (第一報)第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Year and Date: 2008-12-09
• [Presentation] CBP501によるG2チェックポイント阻害作用の詳細についての研究 (2008)
  • Author(s): 松本勇記, 高草木洋一, 新藤陽介, 高草木香織, 小谷野祐希, 佐藤仁志, 河邊拓己, 菅原二三男, 坂口謙吾
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Year and Date: 2008-12-09
• [Presentation] 糖鎖認識機能解明のための分子プローブの合成 (2008)
  • Author(s): 西山和沙, 草柳友恵, 松本勇記, 高草木洋一, 倉持幸司, 菅原二三男, 坂口謙吾, 高橋秀依, 夏苅英昭, 小林進
  • Organizer: 反応と合成の進歩シンポジウム
  • Year and Date: 2008-11-04
• [Presentation] T7ファージディスプレイ法を用いたBrz-2001とチトクロムP450 DWF4の結合解析 (2008)
  • Author(s): 真仁田大輔, 草柳友恵, 高草木洋一, 倉持幸司, 菅原二三男
  • Organizer: 日本ケミカルバイオロジー研究会第3回年会
  • Year and Date: 2008-05-19
• [Presentation] 医薬小分子結合タンパク質の同定を目的としたワンサイクルT7ファージディスプレイ法の研究 (2008)
  • Author(s): 草柳友恵, 高木真奈美, 真仁田大輔, 岩切佳那子, 倉持幸司, 高草木洋一, 中崎敦夫, 小林進, 坂口謙吾, 菅原二三男
  • Organizer: 日本ケミカルバイオロジー研究会第3回年会
  • Year and Date: 2008-05-19
• [Presentation] 糖認識機能解明のための分子プローブの合成 (2008)
  • Author(s): 西山和沙, 草柳友恵, 高草木洋一, 倉持幸司, 菅原二三男, 坂口謙吾, 武内智春, 笠井献一, 高橋秀依, 夏苅英昭, 小林進
  • Organizer: 第55回有機合成化学協会関東支部シンポジウム
  • Year and Date: 2008-05-09
• [Presentation] トリマンノース由来の分子プローブの合成とQCM-PD法による結合ペプチドの探索 (2008)
  • Author(s): 西山和沙, 草柳友恵, 高草木洋一, 倉持幸司, 菅原二三男, 坂口謙吾, 高橋秀依, 夏苅英昭, 小林進
  • Organizer: 日本薬学会128年会
  • Year and Date: 2008-03-27
• [Presentation] ワンステップT7ファージディスプレイ法による医薬小分子の結合タンパク質の同定 (2008)
  • Author(s): 草柳友恵, 高木真奈美1, 真仁田大輔, 岩切佳那子, 倉持幸司, 高草木洋一, 中崎敦夫, 小林進, 坂口謙吾, 菅原二三男
  • Organizer: 日本農芸化学会2008年度大会
  • Year and Date: 2008-03-26
• [Presentation] クルクミン誘導体の抗炎症作用の解析 (2008)
  • Author(s): 西田真之, 吉田優, 東健, 大澤俊彦, 福留啓志, 竹内倫文, 倉持幸司, 菅原二三男, 水品善之, 吉田弘美
  • Organizer: 日本農芸化学会2008年度大会
  • Year and Date: 2008-03-26
• [Presentation] T7ファージディスプレイ法によるポドフィロトキシンの新規結合タンパク質の探索と同定 (2008)
  • Author(s): 斉藤雄己, 倉持幸司, 今井孝彦, 高田慶一, 菅原二三男
  • Organizer: 日本農芸化学会2008年度大会
  • Year and Date: 2008-03-26
• [Presentation] T7 phage display法による抗腫瘍性抗生物質PD 113, 271結合タンパク質の探索 (2008)
  • Author(s): 今井孝彦, 竹内倫文, 石一智, 斉藤雄己, 倉持幸司, 菅原二三男
  • Organizer: 日本農芸化学会2008年度大会
  • Year and Date: 2008-03-26
• [Presentation] T7ファージディスプレイ法によるドキソルビシン標的タンパク質の探索および酵母を用いた生物活性評価法の研究 (2008)
  • Author(s): 真仁田大輔, 小柳邦生, 高橋徳行, 今井孝彦, 石一智, 倉持幸司, 菅原二三男
  • Organizer: 日本農芸化学会2008年度大会
  • Year and Date: 2008-03-26
• [Presentation] 多価不飽和脂肪酸はIMPデヒドロゲナーゼを阻害する (2008)
  • Author(s): 水品善之, 矢中規之, Han, Yunkyung, 竹内倫文, 石丸千里, 菅原二三男, 吉田弘美, 加藤範久
  • Organizer: 日本農芸化学会2008年度大会
  • Year and Date: 2008-03-26
• [Presentation] カバノアナタケから同定された新規アポトーシス誘導物質DDTCTの作用機構 (2007)
  • Author(s): 望月万里, 森田明典, 羽染芳宗, 井田紀子, 村田寛, 船津修, 菅原二三男, 池北雅彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Year and Date: 2007-12-14
• [Presentation] ニンニク由来の含硫黄物質群のDNAポリメラーゼ分子種選択的阻害活性とヒト癌細胞増殖抑制活性BMB2007 (2007)
  • Author(s): 西田真之, 羽田尚彦, 吉田英生, 木元貴士, 倉持幸司, 菅原二三男, 米澤裕子, 栗山磯子, 坂口謙吾, 吉田弘美, 水品善之
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Year and Date: 2007-12-11
• [Presentation] 低分子化合物の高性能な標的探索を可能とするファージディスプレイ法の確立BMB2007 (2007)
  • Author(s): 高草木洋一, 高木真奈美, 草柳友恵, 倉持幸司, 宮野友香, 高草木香織, 菅原二三男, 小林進, 坂口謙吾
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Year and Date: 2007-12-11
• [Presentation] EFFECTS OF DNA POLYMERASE ALPHA POISON DEHYDROALTENUSIN ON THE FISSION YEAST SCHIZOSACCHAROMYCES POMBE BMB2007 (2007)
  • Author(s): 栗山磯子, 紙透伸治, 菅原二三男, 吉田弘美, 水品善之, 野口英史
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Year and Date: 2007-12-11
• [Presentation] カバノアナタケから同定された新規アポトーシス誘導物質DDTCTの作用機構BMB2007 (2007)
  • Author(s): 望月万里, 森田明典, 羽染芳宗, 井田紀子, 村田寛, 船津修, 菅原二三男, 池北雅彦
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Year and Date: 2007-12-11
• [Presentation] 固相有機合成用樹脂を用いたアフィニティービーズの標的タンパク質探索への応用BMB2007 (2007)
  • Author(s): 宮野友香, 倉持幸司, 武内亮, 榎本好宏, 草柳友恵, 高草木洋一, 中崎敦夫, 小林進, 菅原二三男, 坂口謙吾
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会合同大会
  • Year and Date: 2007-12-11
• [Presentation] High-throughput and High-accuracy Drug-Target Identifications (2007)
  • Author(s): Fumio Sugawara, Yoichi Takakusaghi, Kouji Kuramochi
  • Organizer: The 4th Takeda Science Foundation Symposium on PharmaScience: On the Fronthiers of Chemical Biology
  • Year and Date: 2007-12-03
• [Presentation] Down regulation of Tie-2 gene in vivo by novel anti-tumor agent SQAG targeting antiangionesis (2007)
  • Author(s): Yoko Mori, Hiroeki Sahara, Kayo Matsumoto, Nobuaki Takahashi, Takayuki Yamazaki, Keisuke Ohta, Satoko Aoki, Masahiko Miura, Fumio Sugawara, Kengo Sakaguchi, Noriyuki Sato
  • Organizer: 第66回日本癌学会学術総会
  • Year and Date: 2007-10-03
• [Presentation] Identification of cyclosporin A binding protein CSBP that plays a critical role in hepatitis C virus genome replication (2007)
  • Author(s): Hiroeki Sahara, Kengo Morohashi, Koichi Watashi, Yoko Mori, Kazuki Iwabata, Wataru Tsutae, Hiroki Miyashita, Kokichi Kikuchi, Kunitada Shimotohno, Kengo Sakaguchi, Noriyuki Sato, Fumio Sugawara
  • Organizer: 第66回日本癌学会学術総会
  • Year and Date: 2007-10-03
• [Presentation] 抗腫瘍抗生物質fostriecinはプロテインフォスファターゼ2AのCys269-Lys283領域と共有結合する (2007)
  • Author(s): 竹内倫文, 高橋徳行, 石一智, 倉持幸司, 菅原二三男
  • Organizer: 日本ケミカルバイオロジー研究会
  • Year and Date: 2007-05-09
• [Presentation] T7ファージディスプレイ法お用いたポドフィロトキシン結合タンパク質の探索 (2007)
  • Author(s): 斉藤雄己, 倉持幸司, 高田慶一, 菅原二三男
  • Organizer: 日本ケミカルバイオロジー研究会
  • Year and Date: 2007-05-09
• [Presentation] ファージディスプレイ法を用いたneoechinulin Aが活性作用を示す際のターゲットタンパク及び、neoechinulin A生合成に関る遺伝子の探索 (2007)
  • Author(s): 堀内桃子, 竹野正洋, 倉持幸司, 菅原二三男, 鎌倉高志
  • Organizer: 日本ケミカルバイオロジー研究会
  • Year and Date: 2007-05-09
• [Presentation] T7ファージディスプレイ法によるカンプトテシンの新規分子標的hEP1受容体細胞内第3ループの同定 (2007)
  • Author(s): 高草木洋一, 太田慶祐, 倉持幸司, 高草木香織, 小林進, 菅原二三男, 坂口謙吾
  • Organizer: 日本ケミカルバイオロジー研究会
  • Year and Date: 2007-05-09
• [Presentation] T7ファージディスプレイ法によるメトトレキサートの新規結合タンパク質の探索 (2007)
  • Author(s): 黒岩祐貴, 高草木洋一, 倉持幸司, 今井孝彦, 伊藤一明, 吉田充輝, 菅原二三男
  • Organizer: 日本ケミカルバイオロジー研究会
  • Year and Date: 2007-05-09
• [Presentation] T7ファージディスプレイ法によるトラニラスト結合タンパク質の同定 (2007)
  • Author(s): 平山智子, 高草木洋一, 石一智, 倉持幸司, 竹内倫文, 菅原二三男
  • Organizer: 日本ケミカルバイオロジー研究会
  • Year and Date: 2007-05-09
• [Presentation] 有機合成用樹脂をアフィニティー担体として利用したファージディスプレイ法 (2007)
  • Author(s): 榎本好宏, 倉持幸司, 一智, 新井威史, 菅原二三男
  • Organizer: 日本農芸化学会2007年度大会
  • Year and Date: 2007-03-25
• [Presentation] T7ファージディスプレイ法によるポドフィロトキシンの新規結合タンパク質の探索 (2007)
  • Author(s): 斉藤雄己, 高田慶一, 倉持幸司, 菅原二三男
  • Organizer: 日本農芸化学会2007年度大会
  • Year and Date: 2007-03-25
• [Presentation] Aspergione A及びBの提出構造式の訂正と (2007)
  • Author(s): 倉持幸司, 齊藤史代, 中崎敦夫, 高橋徳行, 水品善之, 小林進, 菅原二三男
  • Organizer: Pseudodeflectusinの生物活性日本農芸化学会2007年度大会
  • Year and Date: 2007-03-25
• [Presentation] T7 phage display法を用いたインターフェロン-α結合タンパク質の探索 (2007)
  • Author(s): 今井孝彦, 斉藤雄己, 石一智, 倉持幸司, 渡士幸一, 佐原弘益, 下遠野邦忠, 菅原二三男
  • Organizer: 日本農芸化学会2007年度大会
  • Year and Date: 2007-03-25
• [Book] クラーク分子生物学 (2008)
  • Author(s): 田沼精一,菅原二三男, 他
  • Total Pages: 860
  • Publisher: 丸善