Interaction of the gill, intestine and kidney and integration by endocrine systems in fish osmoregulation

2009 Fiscal Year Final Research Report

• Project/Area Number: 19380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: The University of Tokyo
• Principal Investigator: KANEKO Toyoji The University of Tokyo, 大学院・農学生命科学研究科, 教授 (70221190)
• Project Period (FY): 2007 – 2009
• Keywords: ウナギ / ティラピア / 浸透圧調節 / 鰓 / 塩類細胞 / 腎臓 / 腸

Research Abstract

For a comprehensive understanding of osmoregulatory mechanisms in teleosts, we attempted to clarify cellular and molecular mechanisms of osmoregulation in the gill (chloride cells), intestine and kidney. Considering possible autonomous and hormone-dependent regulations, we indicated that body fluid osmolality in teleosts was controlled by integrated systems equipped with osmosensory mechanisms.

Research Products

• [Journal Article] Acute responses of gill mitochondria-rich cells in Mozambique tilapia Oreochromis mossambicus following transfer from normal freshwater to deionized freshwater. (2010)
  • Author(s): Choi, J.H., Lee, K.M., Inokuchi, M., Kaneko, T.
  • Journal Title: Fish. Sci. 76 Pages: 101-109
  • Peer Reviewed
• [Journal Article] Mg-calcite, a carbonate mineral, constitutes Ca precipitates produced as a byproduct of osmoregulation in the intestine of seawater-acclimated Japanese eel Anguilla japonica. (2010)
  • Author(s): Mekuchi, M., Hatta, T., Kaneko, T.
  • Journal Title: Fish. Sci. 76 Pages: 199-205
  • Peer Reviewed
• [Journal Article] Changes in gene expression levels of somatolactin in the pituitary and morphology of gill mitochondria-rich cells in Mozambique tilapia after transfer to acidic freshwater (pH 3. 5). (2010)
  • Author(s): Furukawa, F., Watanabe, S., Kaneko, T., Uchida, K.
  • Journal Title: Gen. Comp. Endocrinol. (in press)
  • Peer Reviewed
• [Journal Article] Prolactin-releasing peptide receptor expressed in the pituitary in Mozambique tilapia Oreochromis mossambicus: an aspect of prolactin regulatory mechanisms. (2010)
  • Author(s): Watanabe, S, Kaneko, T
  • Journal Title: Gen. Comp. Endocrinol. (in press)
  • Peer Reviewed
• [Journal Article] Spatial, cellular and intracellular localization of Na+/K+-ATPase in the sterically-disposed renal tubules of Japanese eel. (2010)
  • Author(s): Teranishi, K., Kaneko, T.
  • Journal Title: J. Histochem. Cytochem. (in press)
  • Peer Reviewed
• [Journal Article] Osmosensitivity of prolactin cells is enhanced by the water channel aquaporin-3 in a euryhaline Mozambique tilapia (Oreochromis mossambicus). (2009)
  • Author(s): Watanabe, S., Hirano, T., Grau, E.G., Kaneko, T.
  • Journal Title: Am. J. Physiol. Regul. Integr. Comp. Physiol. 296 Pages: R446-R453
  • Peer Reviewed
• [Journal Article] Effects of stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio). (2009)
  • Author(s): Tseng, D.Y., Chou, M.Y., Tseng, Y.C., Hsiao, C.D., Chang-Jen Huang, C.J., Kaneko, T., Hwang, P.P.
  • Journal Title: Am. J. Physiol. Regul. Integr. Comp. Physiol. 296 Pages: R549-R557
  • Peer Reviewed
• [Journal Article] Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia. (2009)
  • Author(s): Inokuchi, M., Hiroi, J., Watanabe, S., Hwang, P.P., Kaneko, T.
  • Journal Title: J. Exp. Biol. 212 Pages: 1003-1010
  • Peer Reviewed
• [Journal Article] Positive buoyancy in eel leptocephali: an adaptation for life in the ocean surface layer. (2009)
  • Author(s): Tsukamoto, K., Yamada, Y., Okamura, A., Kaneko, T., Tanaka, H., Miller, M.J., Horie, N., Mikawa, N., Utoh, T., Tanaka, S.
  • Journal Title: Mar. Biol. 156 Pages: 835-846
  • Peer Reviewed
• [Journal Article] Multiple urea transporter proteins in the kidney of holocephalan elephant fish (Callorhinchus milii). (2009)
  • Author(s): Kakumura, K., Watanabe, S., Bell, J.D., Donald, J.A., Toop, T., Kaneko, T., Hyodo, S.
  • Journal Title: Comp. Biochem. Physiol. B 154 Pages: 239-247
  • Peer Reviewed
• [Journal Article] Ontogenic change in tissue osmolality and developmental sequence of mitochondria-rich cells in Mozambique tilapia developing in freshwater. (2009)
  • Author(s): Yanagie, R., Lee, K.M., Watanabe, S., Kaneko, T.
  • Journal Title: Comp. Biochem. Physiol. A 154 Pages: 263-269
  • Peer Reviewed
• [Journal Article] Morphological changes in gill mitochondria-rich cells in cultured Japanese eel Anguilla japonica acclimated to a wide range of environmental salinity. (2009)
  • Author(s): Seo, M.Y., Lee, K.M., Kaneko, T.
  • Journal Title: Fish. Sci. 75 Pages: 1147-1156
  • Peer Reviewed
• [Journal Article] Na+/H+ exchanger isoform 3 expressed in the apical membrane of gill mitochondrion-rich cells in Mozambique tilapia (Oreochromis mossambicus). (2008)
  • Author(s): Watanabe, S., Niida, M., Maruyama, T., Kaneko, T.
  • Journal Title: Fish. Sci. 74 Pages: 813-821
  • Peer Reviewed
• [Journal Article] Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish. (2008)
  • Author(s): Hiroi, J., Yasumasu, S., McCormick, S.D., Hwang, P.P., Kaneko, T.
  • Journal Title: J. Exp. Biol. 211 Pages: 2584-2599
  • Peer Reviewed
• [Journal Article] Gene expression and morphological localization of NHE3, NCC and NKCC1a in branchial mitochondria-rich cells of Mozambique tilapia (Oreochromis mossambicus) acclimated to a wide range of salinities. (2008)
  • Author(s): Inokuchi, M., Hiroi, J., Watanabe, S., Lee, K.M., Kaneko, T.
  • Journal Title: Comp. Biochem. Physiol. A 151 Pages: 151-158
  • Peer Reviewed
• [Journal Article] Rectal water absorption in seawater-adapted Japanese eel Anguilla japonica. (2008)
  • Author(s): Kim, Y.K., Ideuchi, H., Watanabe, S., Park, S.L., Huh, M.D., Kaneko, T.
  • Journal Title: Comp. Biochem. Physiol. A 151 Pages: 533-541
  • Peer Reviewed
• [Journal Article] Functional morphology of mitochondrion-rich cells in euryhaline and stenohaline teleosts. (2008)
  • Author(s): Kaneko, T., Watanabe, S., Lee, K.M.
  • Journal Title: Aqua-BioSci. Monogr. (ABSM) 1 Pages: 1-62
  • Peer Reviewed
• [Journal Article] Ontogenic change of gill chloride cells in leptocephalus and glass eel stages of the Japanese eel, Anguilla japonica. (2007)
  • Author(s): Sasai, S., Katoh, F., Kaneko, T., Tsukamoto, K.
  • Journal Title: Mar. Biol. 150 Pages: 487-492
  • Peer Reviewed
• [Journal Article] Osmoregulation in elephant fish, Callorhinchus milii (Holocephali), with special reference to the rectal gland. (2007)
  • Author(s): Hyodo, S., Bell, J.D., Healy, J.M., Kaneko, T., Hasegawa, S., Takei, Y., Donald, J.A., Toop, T.
  • Journal Title: J. Exp. Biol. 210 Pages: 1303-1310
  • Peer Reviewed
• [Journal Article] Gene expression of Na+/H+ exchanger in zebrafish H+-ATPase-rich cells during acclimation to low-Na+ and acidic environments. (2007)
  • Author(s): Yan, J.J., Chou, M.Y., Kaneko, T., Hwang, P.P.
  • Journal Title: Am. J. Physiol. Cell Physiol. 293 Pages: C1814-C1823
  • Peer Reviewed
• [Presentation] 魚類の細尿管構造とNa+/K+-ATPaseの局在. (2010)
  • Author(s): 寺西慶太郎・金子豊二
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
  • Year and Date: 20100326-20100330
• [Presentation] ティラピアを淡水から脱イオン水に移行後した際の鰓塩類細胞の経時的変化. (2010)
  • Author(s): 崔丁玄・李慶美・井ノ口繭・金子豊二
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
  • Year and Date: 20100326-20100330
• [Presentation] 様々な塩分環境に馴致したウナギにおける鰓の塩類細胞の形態変化. (2010)
  • Author(s): 徐美暎・李慶美・金子豊二
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
  • Year and Date: 20100326-20100330
• [Presentation] 酸性環境に曝露したティラピアにおける塩類細胞の応答. (2010)
  • Author(s): 古川史也・渡辺壮一・井ノ口繭・金子豊二
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
  • Year and Date: 20100326-20100330
• [Presentation] 魚類が海水適応時に排出する炭酸塩の形成機構. (2010)
  • Author(s): 馬久地みゆき・金子豊二
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
  • Year and Date: 20100326-20100330
• [Presentation] ウナギ仔魚の低塩分環境での飼育:成長と生残. (2009)
  • Author(s): 岡村明浩・山田祥朗・三河直美・堀江則行・金子豊二・田中悟・塚本勝巳
  • Organizer: 平成21年度日本水産学会秋季大会
  • Place of Presentation: 盛岡
  • Year and Date: 20090930-20091003
• [Presentation] ティラピア脳のGnRH3産生ニューロンに対するステロイドホルモンの影響. (2009)
  • Author(s): 倉持麻未・金子豊二・大谷-金子律子
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 20090917-20090920
• [Presentation] 海産魚が形成する炭酸塩は高浸透圧適応において腸内で二価イオンの除去に寄与する. (2009)
  • Author(s): 馬久地みゆき・八田珠郎・金子豊二
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090327-20090331
• [Presentation] 新しい軟骨魚類研究のモデル:ゾウギンザメ. (2009)
  • Author(s): 角村佳吾・渡邊壮一・金子豊二・兵藤晋
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090327-20090331
• [Presentation] 浸透圧応答性プロラクチン分泌調節における浸透圧変化感知機構の解明. (2009)
  • Author(s): 渡邊壮一・平野哲也・E. Gordon Grau・金子豊二
  • Organizer: 平成21年度日本水産学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20090327-20090331
• [Presentation] 魚類の浸透圧調節研究と里山温泉トラフグ. (2009)
  • Author(s): 金子豊二
  • Organizer: 国際水産養殖技術研究者セミナー
  • Place of Presentation: 東京ビックサイト, 東京
  • Year and Date: 2009-07-23
• [Presentation] Seawater-acclimated Japanese eel produce carbonate mineral in the intestine for osmoregulation. (2008)
  • Author(s): Mekuchi, M., Hatta, T., Kaneko, T.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 20081020-20081025
• [Presentation] Eel leptocephali regulate ion and water balances. (2008)
  • Author(s): Lee, K.M., Kaneko, T., Yamada, Y., Okamura A., Tanaka, S., Tsukamoto, K.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 20081020-20081025
• [Presentation] Morphofunctional classification of mitochondria-rich cells in the gills of Mozambique tilapia. (2008)
  • Author(s): Niida-Inokuchi, M., Watanabe, S., Kaneko, T.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Pacifico Yokohama, Yokohama, Japan
  • Year and Date: 20081020-20081025
• [Presentation] Recent advances in chloride cell research. (2008)
  • Author(s): Kaneko, T.
  • Organizer: Pauley Summer Program
  • Place of Presentation: Hawaii Institute of Marine Biology, Coconut Island, Hawaii, USA
  • Year and Date: 2008-07-24
• [Presentation] 機能形態学的アプローチ. (2007)
  • Author(s): 金子豊二
  • Organizer: 魚類の適応と進化の統合生物学:遺伝子から行動まで.
  • Place of Presentation: 東京大学海洋研究所, 東京
  • Year and Date: 20071115-20071116
• [Presentation] Development and differentiation of chloride cells during early life stages of fish. (2007)
  • Author(s): Kaneko, T.
  • Organizer: The 7th International Symposium on Developmental Biotechnology.
  • Place of Presentation: Jeju National University, Jeju, Korea
  • Year and Date: 20071026-20071027
• [Presentation] ティラピア鰓塩類細胞のイオン取り込み機構の解明. (2007)
  • Author(s): 新居田繭・渡辺壮一・廣井準也・金子豊二
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 函館
  • Year and Date: 20070925-20070928
• [Presentation] 硬骨魚類における低浸透圧適応機構としてのプロラクチン分泌および発現調節機構. (2007)
  • Author(s): 渡邊壮一・Gordon E. Grau・平野哲也・金子豊二
  • Organizer: 平成19年度日本水産学会秋季大会
  • Place of Presentation: 函館
  • Year and Date: 20070925-20070928
• [Book] 水圏の生物と生態系,水圏生物科学入門(会田勝美編) (2009)
  • Author(s): 金子豊二・塚本勝巳・津田敦・鈴木譲・佐藤克文
  • Total Pages: 30-83
  • Publisher: 恒星社厚生閣
• [Book] Osmo-and ionoregulation. In“Fish Larval Physiology"(Finn, R. N., Kapoor, B. G. Eds) (2008)
  • Author(s): Kaneko, T., Hiroi, J.
  • Total Pages: 163-183
  • Publisher: Science Publisher Inc.