Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation

2009 Fiscal Year Final Research Report

• Project/Area Number: 19380158
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Kyoto University
• Principal Investigator: MINAMI Naojiro Kyoto University, 大学院・農学研究科, 准教授 (30212236)
• Co-Investigator(Kenkyū-buntansha): KITO Seiji 放射線医学総合研究所, 先端動物実験室, 主任研究員 (20311376)
• Project Period (FY): 2007 – 2009
• Keywords: 発生工学

Research Abstract

In the present study, we investigated the function of oocyte-specific gene, Oog1. The gene translocates to the nucleus of fertilized embryos at the time of zygotic gene activation. In order to analyze the function of the gene, we produced the transgenic mice in which the mRNA of Oog1 is knockdown. In addition, we investigated the protein expression of Oog1 during female meiosis and it is revealed that the protein exists in the oocyte nucleus at the prophase of meiosis. In another experiments, we identified the regulatory regions of Oog1 which controls the expression of the gene.

Research Products

• [Journal Article] Cell-Free Extracts from Mammalian Oocytes Partially Induce Nuclear Reprogramming in Somatic Cells. (2009)
  • Author(s): Miyamoto K, Tsukiyama T, Yang Y, Li N, Minami N, Yamada M, Imai H.
  • Journal Title: Biol Reprod 80(5) Pages: 935-43
• [Journal Article] In vitro fertilization in inbred BALB/c mice: osmotic pressure and calcium are important factors for penetration ofperm through the zona pellucida. (2008)
  • Author(s): Kito, S, Ohta, Y
  • Journal Title: Zygote 16 Pages: 249-257
• [Journal Article] Zygotic gene activation and maternal factors in mammals. (2007)
  • Author(s): Minami, N. Suzuki, T., Tsukamoto, S
  • Journal Title: J Reprod Dev 53(4) Pages: 707-15
• [Presentation] 初期胚の発生と遺伝子 (2009)
  • Author(s): 南直治郎
  • Organizer: 第27回受精着床学会教育講演
  • Place of Presentation: 京都
  • Year and Date: 20090000
• [Presentation] Z P3 promoter is not enough to analyze the early expressing genes during oogenesis, such as Oog1 Analysis of Oog1, an oocyte-specific gene, using transgenic RNAi approach (2009)
  • Author(s): Hisashi Imaichi, Eriko Okazaki, Satoshi Tsukamoto, Yuki Ohta, Seiji Kito, Koji Kimura, Hiroshi Imai, Naojiro Minami
  • Organizer: 42nd Society for the Study of Reproduction
  • Place of Presentation: Pittsburgh, USA
  • Year and Date: 20090000
• [Book] Identification and Expression Analysis of Small RNAs During Development.(Methods Mol Bio) (2008)
  • Author(s): Watanabe, T., Imai, H., Minami N.
  • Total Pages: 173-185