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2009 Fiscal Year Final Research Report

Elucidation of the molecular mechanism underlying the enhancement of somatic-cell reprogramming by a histone deacetylase inhibitor, and its applications

Research Project

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Project/Area Number 19380161
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKinki University

Principal Investigator

KISHIGAMI Satoshi  Kinki University, 生物理工学部, 准教授 (10291064)

Research Collaborator TERUHIKO Wakayama  理化学研究所・神戸研究所, チームリーダー (40360672)
KAZUHIRO Saeki  近畿大学, 生物理工学部, 教授 (10298937)
Project Period (FY) 2007 – 2009
Keywords発生工学 / 核の初期化
Research Abstract

Somatic-cell nuclear transfer (SCNT) has been extremely inefficient with rates less than 5 percentage points since the first clone, "Dolly" the sheep. The representative researcher for this grant found that treatment of cloned embryos with trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), dramatically increase the production rate of cloned mice more than six-times. Supported by this grant, the importance of HDAC in embryonic development and a mechanism underlying improvement of SCNT by HDACi treatment were revealed and, further cloning of "unclonable" mouse strains were achieved for the first time.

  • Research Products

    (33 results)

All 2010 2009 2008 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (14 results) Book (9 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] How to improve the success rate of mouse cloning technology2010

    • Author(s)
      Thuan NV, Kishigami S, Wakayama T
    • Journal Title

      J Reprod Dev Vol.56,No.2

      Pages: 20-30

    • Peer Reviewed
  • [Journal Article] The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice2009

    • Author(s)
      Van Thuan N, Bui HT, Kim JH, Hikichi T, Wakayama S, Kishigami S, Mizutani E, Wakayama T
    • Journal Title

      Reproduction Vol.138,No.2

      Pages: 309-317

    • Peer Reviewed
  • [Journal Article] The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear reprogramming2008

    • Author(s)
      Bui HT, Wakayama S, Kishigami S, Kim JH, Van Thuan N, Wakayama T
    • Journal Title

      Development Vol.135,No.23

      Pages: 3935-3945

    • Peer Reviewed
  • [Journal Article] Somatic cell nuclear transfer: infinite reproduction of a unique diploid genome2008

    • Author(s)
      Kishigami S, Wakayama S, Hosoi Y, Iritani A, Wakayama T
    • Journal Title

      Exp Cell Res Vol.314,No.9

      Pages: 1945-1950

    • Peer Reviewed
  • [Journal Article] Efficient strontium-induced activation of mouse oocytes in standard culture media by chelating calcium2007

    • Author(s)
      Kishigami S, Wakayama T
    • Journal Title

      J Reprod Dev Vol.53,No.6

      Pages: 1207-1215

    • Peer Reviewed
  • [Journal Article] Regulation of chromatin and chromosome morphology by histone H3 modifications in pig oocytes2007

    • Author(s)
      Bui HT, Van Thuan N, Kishigami S, Wakayama S, Hikichi T, Ohta H, Mizutani E, Yamaoka E, Wakayama T, Miyano T
    • Journal Title

      Reproduction Vol.133,No.2

      Pages: 371-382

    • Peer Reviewed
  • [Journal Article] Successful mouse cloning of an outbred strain by trichostatin A treatment after somatic nuclear transfer.2007

    • Author(s)
      Kishigami S, et al.
    • Journal Title

      J Reprod Dev Vol.53,No.1

      Pages: 165-170

    • Peer Reviewed
  • [Journal Article] Differentiation potential of parthenogenetic embryonic stem cells is improved by nuclear transfer2007

    • Author(s)
      Hikichi T, Wakayama S, Mizutani E, Takashima Y, Kishigami S, Van Thuan N, Ohta H, Thuy Bui H, Nishikawa S, Wakayama T
    • Journal Title

      Stem Cells Vol.25,No.1

      Pages: 46-53

    • Peer Reviewed
  • [Journal Article] Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes2007

    • Author(s)
      Wakayama S, Suetsugu R, Thuan NV, Ohta H, Kishigami S, Wakayama T
    • Journal Title

      Curr Biol Vol.17,No.4

      Pages: R120-121

    • Peer Reviewed
  • [Presentation] A unique property of specified mouse blastomere in Cdx2 expression2009

    • Author(s)
      Kishigami S., et al.
    • Organizer
      6th Asian Reproductive Biotechnology Conference
    • Place of Presentation
      Siem Reap, Cambodia
    • Year and Date
      2009-11-24
  • [Presentation] The impact of EGTA as chelating calcium on oocyte activation in mice2008

    • Author(s)
      Tsujimoto Y., et al.
    • Organizer
      5th Asian Reproductive Biotechnology Conference
    • Place of Presentation
      Yunnan, China
    • Year and Date
      20081126-20081130
  • [Presentation] ウシ体細胞核移植胚におけるトリコスタチンA処理の至適時間の検討2008

    • Author(s)
      高橋千明, ら
    • Organizer
      平成20年度(第58回大会)関西畜産学会
    • Place of Presentation
      神戸市
    • Year and Date
      20080902-20080903
  • [Presentation] Effects of trichostatin A on gene expression and in-vitro development of bovine embryos cloned from transfected fibroblasts carrying a luciferase gene.2008

    • Author(s)
      Iwamoto D, et al.
    • Organizer
      16th International Congress on Animal Reproduction
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      20080713-20080717
  • [Presentation] Effects of trichostatin A on DNA methylation in cloned bovine embryos.2008

    • Author(s)
      Iwamoto D, et al.
    • Organizer
      34th Annual Conference of International Embryo Transfer Society
    • Place of Presentation
      Denver, USA
    • Year and Date
      20080105-20080109
  • [Presentation] オーダーメイド胚培養:移植される核の種類に適した体外培養を考える2008

    • Author(s)
      岸上哲士
    • Organizer
      第11回日本IVF学会
    • Place of Presentation
      大阪
    • Year and Date
      2008-10-14
  • [Presentation] Somatic cell nuclear transfer with HDACi2008

    • Author(s)
      Kishigami S
    • Organizer
      4th JSAR-KSAR Joint Symposium
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2008-06-20
  • [Presentation] 発生工学におけるドラッグディスカバリー:クローン技術改善への試み2007

    • Author(s)
      岸上哲士
    • Organizer
      発生工・疾患モデル研究会
    • Place of Presentation
      東京
    • Year and Date
      20071000
  • [Presentation] Histone deacetylases: key regulators to determine the efficiency of reprogramming after somatic-cell nuclear transfer2007

    • Author(s)
      Kishigami S
    • Organizer
      5th Anniversary Congress of International Drug Discovery Science & Technology (IDDST)
    • Place of Presentation
      Shanghai, China
    • Year and Date
      20070500
  • [Presentation] Abnormal cell adhesion in cloned mouse embryos2007

    • Author(s)
      Suetsugu, S., et al.
    • Organizer
      第40回日本発生生物学会大会(細胞生物学会合同)
    • Place of Presentation
      福岡国際会議場、福岡
    • Year and Date
      20070500
  • [Presentation] Effects of trichostatin A on development of bovine somatic cell nuclear transfer embryos.2007

    • Author(s)
      Iwamoto D, et al.
    • Organizer
      33th Annual Conference of International Embryo Transfer Society
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20070106-20070110
  • [Presentation] Success of mouse cloning from an outbred strain by trichostatin a treatment after somatic nuclear transfer2007

    • Author(s)
      Kishigami S., et al.
    • Organizer
      3rd Annual Conference of the International Embryo Transfer Society (IETS)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      20070106-20070110
  • [Presentation] Efficient strontium-induced activation of mouse oocytes in standard culture media by chelating calcium2007

    • Author(s)
      Kishigami S., Wakayama T
    • Organizer
      4th Asian Reproductive Biotechnology Conference
    • Place of Presentation
      Singapore
    • Year and Date
      2007-11-24
  • [Presentation] Oocyte activation by strontium in the presence of calcium supports full- term development of somatic cell cloned embryos2007

    • Author(s)
      Kishigami S., Wakayama T
    • Organizer
      40th Annual meeting of the Society of Reproduction (SSR)
    • Place of Presentation
      San Antonio, TX, USA
    • Year and Date
      2007-07-21
  • [Book] Nuclear Transfer with HDACi as a New Cloning Method((Lucas T. Dahnof編))2010

    • Author(s)
      Satoshi Kishigami, Daisaku Iwamoto, Kazuhiro Saeki, Kazuya Matsumoto, Akira Iritani, Yoshihiko Hosoi著
    • Total Pages
      329-334
    • Publisher
      Nova Science Pub Inc
  • [Book] 体細胞核移植におけるリプログラミング促進技術の開発2009

    • Author(s)
      岸上哲士, 矢持隆之, 松原圭吾, 細井美彦著
    • Total Pages
      43-46
    • Publisher
      メディカルレビュー社
  • [Book] 核移植とiPS細胞技術の比較:リプログラミングの効率化を目指して2009

    • Author(s)
      岸上哲士, 辻本賀子, 矢持隆之, 細井美彦著
    • Total Pages
      223-227
    • Publisher
      秀潤社
  • [Book] Gene knockout Protocols (Methods in Molecular Biologyシリーズ, Vol. 530) (Wolfgang Wurst編)2009

    • Author(s)
      Sayaka Wakayama, Satoshi Kishigami, Teruhiko Wakayama著
    • Total Pages
      516
    • Publisher
      Humana Press
  • [Book] 体細胞核移植におけるリプログラミング促進技術の開発2008

    • Author(s)
      岸上哲士, 細井美彦著
    • Total Pages
      270-273
    • Publisher
      メディカルレビュー社
  • [Book] Microinjection: Methods and Applications (Methods in Molecular Biologyシリーズ(David Carrol編)2008

    • Author(s)
      Satoshi Kishigami, Teruhiko Wakayama著
    • Total Pages
      224
    • Publisher
      Humana Press
  • [Book] Genetic and Epigenetic Control of Mammalian Germ Cell Development and Function (Masami Nozaki編)2008

    • Author(s)
      Satoshi Kishigami, Hiroshi Ohta, Teruhiko Wakayama
    • Total Pages
      133-152
  • [Book] Progress in DNA Methylation Research (Hans P. Neumann編)2008

    • Author(s)
      Satoshi Kishigami著
    • Total Pages
      256
    • Publisher
      Nova Science Publishers Inc
  • [Book] 体細胞核移植クローンとクローンES細胞の樹立2007

    • Author(s)
      若山静香, 岸上哲士, 若山照彦著
    • Total Pages
      2197-2202
    • Publisher
      共立出版
  • [Patent(Industrial Property Rights)] 核移植卵子の作製方法2006

    • Inventor(s)
      岸上哲士
    • Industrial Property Rights Holder
      独立行政法人理化学研究所、学校法人近畿大学,独立行政法人科学技術振興機構
    • Industrial Property Number
      PCT出願・PCT/JP2006/319311
    • Filing Date
      2006-09-28

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Published: 2011-06-18   Modified: 2016-04-21  

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