2009 Fiscal Year Final Research Report
Development of autologous bone marrow transplantation therapy using hematopoietic stem cells in dogs
| Project/Area Number |
19380175
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TSUJIMOTO Hajime The University of Tokyo, 大学院・農学生命科学研究科, 教授 (60163804)
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| Co-Investigator(Kenkyū-buntansha) |
OHNO Koichi 東京大学, 大学院・農学生命科学研究科, 准教授 (90294660)
FUJINO Yasuhito 東京大学, 大学院・農学生命科学研究科, 助教 (70401180)
GOTO Yuko 東京大学, 大学院・農学生命科学研究科, 特任助教 (80436518)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 犬 / 化学療法 / 骨髄移植 / CD34 / 造血幹細胞 / リンパ腫 |
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| Research Abstract |
To advance antineoplastic therapies for lymphohematopoietic tumors that are common in dogs, we developed a series of systems for autologous bone marrow transplantation. Optimal conditions for bone marrow cell collection, hematopoietic cell separation, and their cryopreservation were obtained. Next, hematopoietic stem cells resistant to antineoplastic agents were generated by a gene transfer method. Finally, we developed a higly sensitive quantitative monitoring system of minimal residual disease to assess the treatment efficacy. The present study provided a basis for the development of antineoplastic therapies with excellent treatment outcomes.
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[Journal Article] Quantitative analysis of mRNA for 10 different drug resistance factors in dogs with lymphoma.2010
Author(s)
Tomiyasu, H., Goto-Koshino, Y., Takahashi, M., Fujino, Y., Ohno, K., Tsujimoto, H.
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Journal Title
Peer Reviewed
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[Journal Article] Monitoring of minimal residual disease (MRD) after multidrug chemotherapy and its correlation to outcome in dogs with lymphoma: A proof-of-concept pilot study.2010
Author(s)
Yamazaki, J., Takahashi, M., Setoguchi, A., Fujino, Y., Ohno, K., Tsujimoto, H.
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Journal Title
J. Vet. Intern. Med. (印刷中)
Peer Reviewed
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[Journal Article] Semsitivity for the detection of a clonally rearranged antigen receptor gene in endoscopically obtained biopsy specimens from canine alimentary lymphoma.2009
Author(s)
Fukushima, K., Ohno, K., Goto-Koshino, Y., Uchida, K., Nomura, K., Takahashi, M., Nakashima, K., Fujino, Y., Tsujimoto, H.
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Journal Title
J. Vet. Med. Sci. 71
Pages: 1673-1676
Peer Reviewed
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[Journal Article] Identification of cancer stem cells in a Tax-transgenic (Tax-Tg) mouse model of adult T-cell leukemia/lymphoma (ATL).2009
Author(s)
Yamazaki, J., Mizukami, T., Takizawa, K., Kuramitsu, M., Momose, H., Masumi, A., Ami, Y., Hasegawa, H., Hall, W.W., Tsujimoto, H., Hamaguchi, I., Yamaguchi, K.
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Journal Title
Blood 114
Pages: 2709-2720
Peer Reviewed
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[Journal Article] Quantitative analysis of mRNA transcripts of Hox, SHH, PTCH, Wnt, and Fzd genes in canine hematopoietic progenitor cells and various in vitro colonies differentiated from the cells.2009
Author(s)
Ide, K., Goto-Koshino, Y., Momoi, Y., Fujino, Y., Ohno, K., Tsujimoto, H.
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Journal Title
J. Vet. Med. Sci. 71
Pages: 69-77
Peer Reviewed
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[Journal Article] Quantitative assessment of minimal residual disease (MRD) in canine lymphoma by using real-time polymerase chain reaction.2008
Author(s)
Yamazaki, J., Baba, K., Goto-Koshino, Y., Setoguchi-Mukai, A., Fujino, Y., Ohno, K., Tsujimoto, H.
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Journal Title
Vet. Immunol. Immunopathol. 126
Pages: 321-331
Peer Reviewed
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[Journal Article] Induction of chemoresistance in a canine cultured cell line by retroviral transduction of the canine multidrug resistance 1 gene (mdr1).2007
Author(s)
Matsuura, S., Koto, H., Ide, K., Fujino, Y., Setoguchi-Mukai, A., Ohno, K., Tsujimoto, H.
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Journal Title
Am. J. Vet. Res. 68
Pages: 95-100
Peer Reviewed
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