2009 Fiscal Year Final Research Report
Creation of plants that efficiently absorb and remove formaldehyde in air by genetic engineering, and its application to ornamental foliage plants
| Project/Area Number |
19380186
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Kinki University |
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Principal Investigator |
IZUI Katsura Kinki University, 生物理工学部, 教授 (20025414)
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| Co-Investigator(Kenkyū-buntansha) |
AKITA Motomu 近畿大学, 生物理工学部, 准教授 (80258061)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ホルムアルデヒド / シックハウスガス / 形質転換観葉植物 / ファイトレメディエーション / メタノール資化性菌 / リブロースモノリン酸経路 / コドン最適化合成DNA / マイクロアレィ解析 |
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| Research Abstract |
The two enzymes which are involved in the formaldehyde (HCHO) fixation in a methanol utilizing bacterium were expressed both in Arabidopsis (At) and tobacco. The obtained transformants showed an augmented capacity of absorbing HCHO and an enhanced tolerance to HCHO. Genome-wide analysis of the genes whose expression were affected in response to HCHO, gave clues to the mechanisms of toxicity of and tolerance to HCHO. The plasmids for the efficient expression of these enzyme genes in plants were constructed, and trials were made to obtain transgenic ornamental plants which will be useful for phytoremediation of HCHO-polluted indoor air.
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Research Products
(9 results)
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[Journal Article] Assimilation of formaldehyde in transgenic plants due to the introduction of bacterial ribulose monophosphate pathway genes.2010
Author(s)
Chen, L.M., Yurimoto, H., Li, K.Z., Orita, I., Akita, M., Kato, N., Sakai, Y., Izui, K.
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Journal Title
Biosci. Biotechnol. Biochem 74(3)
Pages: 627-635
Peer Reviewed
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[Journal Article] Purification capability of tobacco transformed with enzymes from a methylotrophic bacterium for formaldehyde.2007
Author(s)
Sawada A., Oyabu T., Chen, L.M., Li, K.Z., Hirai, N., Yurimoto, H., Orita, I., Sakai, Y., Kato, N., Izui, K.
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Journal Title
Int. J. Phytoremediation 9
Pages: 487-496
Peer Reviewed
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[Presentation] Transcription analysis of formaldehyde response in wild-type and formaldehyde-tolerant transformant of Arabidopsis2010
Author(s)
Kubo, S., Chen, L.M., Sakakibara, H., Orita, I., Yurimoto, H., Kato, N., Sakai, Y., Akita, M., Izui, K.
Organizer
ICAR2010 (International Congress of Arabidopsis Research)
Place of Presentation
Pacifico, Yokohama
Year and Date
20100606-20100610
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