2009 Fiscal Year Final Research Report
Bacterial community of crops by molecular biological methods
Project/Area Number |
19380187
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Boundary agriculture
|
Research Institution | National Institute for Agro-Environmental Sciences |
Principal Investigator |
TSUSHIMA Seiya National Institute for Agro-Environmental Sciences, 農業環境インベントリーセンター, センター長 (50354080)
|
Co-Investigator(Kenkyū-buntansha) |
TSUCHIYA Kenichi 九州大学, 大学院・農学研究院, 教授 (40150510)
|
Project Period (FY) |
2007 – 2009
|
Keywords | 生物環境 / 葉圏細菌 |
Research Abstract |
There are many unculturable bacteria in environment, for example, ca.99% of bacteria in soil have been reported to be unculturable. However, little is known about bacteria on plant surface. In this study, the rapid and simple counting method of total bacteria on plant surface was developed and the populations of culturable (A) and unculturable (B) bacteria on the leaf surface of crops (rice and wheat) were assessed by the developed counting method and agar medium. The proportions (A/B) of their bacteria of rice and wheat before heading time were about 1/10 and 1/1000, respectively, indicating the significant difference in the proportion(A/B) among the two crops. The results suggest the dynamic change in the proportion of culturable bacteria on leaf surface among crops.
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