2009 Fiscal Year Final Research Report
Development of novel analytical methods for phosphoproteomics by using a phosphate-binding tag molecule.
| Project/Area Number |
19390011
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
KOIKE Tohru Hiroshima University, 大学院・医歯薬学総合研究科, 教授 (90186586)
|
|---|
| Project Period (FY) |
2007 – 2009
|
| Keywords | リン酸化プロテオミクス / ケミカルバイオロジー / リン酸化蛋白質 / キナーゼ / フォスファターゼ |
|---|
| Research Abstract |
By utilizing the Phos-tag molecule and its derivatives, we have developed convenient and reliable methods for the detection and separation of phosphorylated peptides and proteins. In this program, we applied the Phos-tag technology to (1) FRET system for kinase profiling using Phos-tag, (2) phosphate affinity electrophoresis, (3) phosphate affinity column system. We believe that our Phos-tag technology will result in great progress in phosphoproteomics.
|
-
-
-
-
-
-
-
[Journal Article] FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway2008
Author(s)
M. Ishiai, H. Kitao, A. Smogorzewska, J, Tomida, A. Kinomura, E. Uchida, A. Saberi, E. Kinoshita, E. Kinoshita-Kikuta, T. Koike, S. Tashiro, S.J. Elledge, M. Takata
-
Journal Title
Nature Structural & Molecular Biology 15
Pages: 1138-1146
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
