Elucidation of function of polyamines and regulation of their contents in cells

2009 Fiscal Year Final Research Report

• Project/Area Number: 19390016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Chiba University
• Principal Investigator: IGARASHI Kazuei Chiba University, 名誉教授 (60089597)
• Co-Investigator(Kenkyū-buntansha): NISHIMURA Kazuhiro 千葉大学, 大学院・薬学研究院, 講師 (60302569) ; KASHIWAGI Keiko 千葉科学大学, 薬学部, 教授 (80169424)
• Project Period (FY): 2007 – 2009
• Keywords: 核酸 / 生体分子 / 生理活性 / 脳・神経 / バイオテクノロジー

Research Abstract

Polyamines (putrescine, spermidine, spermine) play important roles in cell proliferation and differentiation. In this study, role of polyamines on stimulation of specific kinds of protein synthesis and NMDA receptor activity at both stimulatory and resting states, and properties of polyamine transport systems in Escherichia coli and yeast have been studied. New findings in this study have been described below.

Research Products

• [Journal Article] Intense correlation between protein-conjugated acrolein and primary Sjogren's syndrome (2010)
  • Author(s): Higashi, K., Yoshida, M., Igarashi, A., Ito, K., Wada, Y., Murakami, S., Kobayashi, D., Nakano, M., Sohda, M., Nakajima, T., Narita, I., Toida, T., Kashiwagi, K., Igarashi, K.
  • Journal Title: Clin.Chim.Acta 41 Pages: 359-363
  • Peer Reviewed
• [Journal Article] Identification of acrolein-conjugated protein in plasma of patients with brain infarction (2010)
  • Author(s): Yoshida, M., Higashi, K., Jin, L., Machi, Y., Suzuki, T., Masuda, A., Dohmae, N., Suganami, A., Tamura, Y., Nishimura, K., Toida, T., Tomitori, H., Kashiwagi, K., Igarashi, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 391 Pages: 1234-1239
  • Peer Reviewed
• [Journal Article] Modulation of cellular function by polyamines (2010)
  • Author(s): Igarashi, K., Kashiwagi, K.
  • Journal Title: Int.J.Biochem.Cell Biol. 42 Pages: 39-51
  • Peer Reviewed
• [Journal Article] Polyamine-modulon in yeast―stimulation of COX4 synthesis by spermidine at the level of translation (2009)
  • Author(s): Uemura, T., Higashi, K., Takigawa, M., Toida, T., Kashiwagi, K., Igarashi, K.
  • Journal Title: Int.J.Biochem.Cell Biol. 41 Pages: 2538-2545
  • Peer Reviewed
• [Journal Article] Intense correlation between brain infarction and protein-conjugated acrolein (2009)
  • Author(s): Saiki, R., Nishimura, K., Ishii, I., Omura, T., Okuyama, S., Kashiwagi, K., Igarashi, K.
  • Journal Title: Stroke 40 Pages: 3356-3361
  • Peer Reviewed
• [Journal Article] Acrolein, IL-6 and CRP as markers of silent brain infarction (2009)
  • Author(s): Yoshida, M., Tomitori, H., Machi, Y., Katagiri, D., Ueda, S., Horiguchi, K., Kobayashi, E., Saeki, N., Nishimura, K., Ishii, I., Kashiwagi, K., Igarashi, K.
  • Journal Title: Atherosclerosis 203 Pages: 557-562
  • Peer Reviewed
• [Journal Article] Acrolein toxicity : comparison with reactive oxygen species (2009)
  • Author(s): Yoshida, M., Tomitori, H., Machi, Y., Hagihara, M., Higashi, K., Goda, H., Ohya, T., Niitsu, M., Kashiwagi, K., Igarashi, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 378 Pages: 313-318
  • Peer Reviewed
• [Journal Article] Identification of proteins whose synthesis is preferentially enhanced by polyamines at the level of translation in mammalian cells (2009)
  • Author(s): Nishimura, K., Okudaira, H., Ochiai, E., Higashi, K., Kaneko, M., Ishii, I., Nishimura, T., Dohmae, N., Kashiwagi, K., Igarashi, K.
  • Journal Title: Int.J.Biochem.Cell Biol. 41 Pages: 2251-2261
  • Peer Reviewed
• [Journal Article] Enhancement of the synthesis of RpoE and StpA by polyamines at the level of translation in Escherichia coli under heat shock conditions (2009)
  • Author(s): Terui, Y., Higashi, K., Tabei, Y., Tomitori, H., Yamamoto, K., Ishihama, A., Igarashi, K., Kashiwagi, K.
  • Journal Title: J.Bacteriol. 191 Pages: 5348-5357
  • Peer Reviewed
• [Journal Article] Selective structural change by spermidine of the bulged-out region of double-stranded RNA and its effect on RNA function (2008)
  • Author(s): Higashi, K., Terui, Y., Suganami, A., Tamura, Y., Nishimura, K., Kashiwagi, K., Igarashi, K.
  • Journal Title: J.Biol.Chem. 283 Pages: 32989-32994
  • Peer Reviewed
• [Journal Article] The pore region of N-methyl-D-aspartate receptors differentially influences stimulation and block by spermine (2008)
  • Author(s): Jin, L., Miyazaki, M., Mizuno, S., Takigawa, M., Hirose, T., Nishimura, K., Toida, T., Williams, K., Kashiwagi, K., Igarashi, K.
  • Journal Title: J.Pharmacol.Exp.Ther. 327 Pages: 68-77
  • Peer Reviewed
• [Journal Article] Selective structural change of bulged-out region of double-stranded RNA containing bulged nucleotides by spermidine (2008)
  • Author(s): Higashi, K., Terui, Y., Inomata, E., Katagiri, D., Nomura, Y., Someya, T., Nishimura, K., Kashiwagi, K., Kawai, G., Igarashi, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 370 Pages: 572-577
  • Peer Reviewed
• [Journal Article] Binding of spermine and ifenprodil to a purified, soluble regulatory domain of the N-methyl-Daspartate receptor (2008)
  • Author(s): Han, X., Tomitori, H., Mizuno, S., Higashi, K., Full, C., Fukiwake, T., Terui, Y., Leewanich, P., Nishimura, K., Toida, T., Williams, K., Kashiwagi, K., Igarashi, K.
  • Journal Title: J.Neurochem. 107 Pages: 1566-1577
  • Peer Reviewed
• [Journal Article] Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli (2008)
  • Author(s): Higashi, K., Ishigure, H., Demizu, R., Uemura, T., Nishino, K., Yamaguchi, A., Kashiwagi, K., Igarashi, K.
  • Journal Title: J.Bacteriol. 190 Pages: 872-878
  • Peer Reviewed
• [Journal Article] Polyamine uptake by DUR3 and SAM3 in Saccharomyces cerevisiae (2007)
  • Author(s): Uemura, T., Kashiwagi, K., Igarashi, K.
  • Journal Title: J.Biol.Chem. 282 Pages: 7733-7741
  • Peer Reviewed
• [Journal Article] Enhancement of the synthesis of RpoN, Cra and H-NS by polyamines at the level of translation in Escherichia coli cultured with glucose and glutamate (2007)
  • Author(s): Terui, Y., Higashi, K., Taniguchi, S., Shigemasa, A., Nishimura, K., Yamamoto, K., Kashiwagi, K., Ishihama, A., Igarashi, K.
  • Journal Title: J.Bacteriol. 189 Pages: 2359-2368
  • Peer Reviewed
• [Journal Article] Comparative studies of anthraquinone-and anthracenetetraamines as blockers of N-methyl-D-aspartate receptors (2007)
  • Author(s): Jin, L., Sugiyama, H., Takigawa, M., Katagiri, D., Tomitori, H., Nishimura, K., Kaur, N., Phanstiel IV, O., Kitajima, M., Takayama, H., Okawara, T., Williams, K., Kashiwagi, K., Igarashi, K.
  • Journal Title: J.Pharmacol.Exp.Ther. 320 Pages: 47-55
  • Peer Reviewed
• [Journal Article] Minor contribution of an internal ribosome entry site in the 5'-UTR of ornithine decarboxylase mRNA on its translation (2007)
  • Author(s): Nishimura, K., Sakuma, A., Yamashita, T., Hirokawa, G., Imataka, H., Kashiwagi, K., Igarashi, K.
  • Journal Title: Biochem.Biophys.Res.Commun. 364 Pages: 124-130
  • Peer Reviewed
• [Journal Article] Anthraquinone polyamines : novel channel blockers of N-methyl-D-aspartate receptors (2007)
  • Author(s): Kashiwagi, K., Williams, K., Igarashi, K.
  • Journal Title: Amino Acids 33 Pages: 299-304
  • Peer Reviewed
• [Journal Article] Characteristics of cellular polyamine transport in prokaryotes and eukaryotes
  • Author(s): Igarashi, K., Kashiwagi, K.
  • Journal Title: Plant Physiol.Biochem. (in press)
  • Peer Reviewed
• [Presentation] Binding of spermine and ifenprodil to a regulatory domain (R-domain) of the N-methyl-D-aspartate (NMDA) receptor and the role of R-domain on the activity of NMDA receptor (2009)
  • Author(s): Kashiwagi, K., Han, X., Mizuno, S., Tomitori, H., Higashi, K., Terui, Y., Nishimura, K., Toida, T., Williams, K., Igarashi, K.
  • Organizer: 第31回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 大阪大学コンベンションセンター場
  • Year and Date: 2009-12-01
• [Presentation] 脳梗塞時のアクロレイン産生におけるトランスグルタミナーゼ2の役割 (2009)
  • Author(s): 斎木遼太郎, 東恭平, 石井伊都子, 西村和洋, 戸井田敏彦, 辰川英樹, 小嶋聡一, 柏木敬子, 五十嵐一衛
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-23
• [Presentation] ポリアミン酸化により産生されるアクロレインの毒性 : 活性酸素種との比較 (2009)
  • Author(s): 富取秀行, 吉田円, 町佳樹, 萩原基文, 東恭平, 合田ひとみ, 大谷武司, 新津勝, 柏木敬子, 五十嵐一衛
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2009-10-23
• [Presentation] Polyamine effect on protein synthesis and its mechanism (2009)
  • Author(s): Kashiwagi, K., Terui, Y., Higashi, K., Okudaira, H., Ochiai, E., Tomitori, H., Nishimura, K., Yamamoto, K., Ishihama, A., Igarashi, K.
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
  • Year and Date: 2009-06-22
• [Presentation] Acrolein as biomarkers in stroke (2009)
  • Author(s): Igarashi, K.
  • Organizer: Biogenic Amines : Biochemical, Physiological, and Clinical Aspects
  • Place of Presentation: Bertinoro, Italy
  • Year and Date: 2009-05-12
• [Presentation] スペルミン及びイフェンプロジルのNMDA受容体調節領域への結合能 (2009)
  • Author(s): 韓霞, 富取秀行, 水野聡美, 東恭平, 吹譯友秀, 照井祐介, 西村和洋, 戸井田敏彦, 柏木敬子, 五十嵐一衛
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] 細胞周期進行におけるポリアミンの役割解析 (2009)
  • Author(s): 柏木敬子, 山下智子, 西村和洋, 奥平宏之, 當銘真悠子, 東恭平, 藤原邦雄, 五十嵐一衛
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-03-26
• [Presentation] 大腸菌 OppA mRNA及びラット肝Ile-tRNAにおける二重鎖RNAのバルジ構造のスペルミンによる選択的な構造変化 (2008)
  • Author(s): 東恭平, 菅波晃子, 田村裕, 照井裕介, 西村和洋, 柏木敬子, 五十嵐一衛
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-12
• [Presentation] 大腸菌スペルミジン優先取り込み系の膜貫通蛋白質 PotB及び PotCの性質 (2008)
  • Author(s): 出水梨沙, 東恭平, 酒巻善春, 植村武史, 戸来江美子, 善田理沙, 西村和洋, 柏木敬子, 五十嵐一衛
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2008-12-09
• [Presentation] スペルミンによるNMDA受容体活性促進効果とチャネルブロック作用に影響するチャネル領域の同定 (2008)
  • Author(s): 柏木敬子, 金麗花, 宮崎真紀, 水野聡美, 瀧川美紀, 廣瀬直雄, 西村和洋, 戸井田敏彦, K. Williams, 五十嵐一衛
  • Organizer: 第30回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-08-08
• [Presentation] NMDA受容体の膜領域の構造の解析 (2008)
  • Author(s): 金麗花, 田村真紀, 瀧川美紀, 廣瀬直雄, 西村和洋, 戸井田敏彦, K. Williams, 柏木敬子, 五十嵐一衛
  • Organizer: 日本薬学会第128年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-03-28
• [Presentation] 動物細胞のポリアミンにより翻訳レベルで合成促進を受ける蛋白質の同定 (2007)
  • Author(s): 落合絵里子, 奥平宏之, 西村和洋, 柏木敬子, 堂前直, 西村友枝, 瀧尾擴士, 五十嵐一衛
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会合同大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-13
• [Presentation] Acrolein toxicity : its mechanism and comparison of toxicity with reactive oxygen species (2007)
  • Author(s): Yoshida, M., Hagihara, M., Tomitori, H., Nishimura, K., Kashiwagi, K., Igarashi, K.
  • Organizer: International Congress on Biogenic Amines : Biological and Clinical Perspectives
  • Place of Presentation: Sicily, Italy
  • Year and Date: 2007-10-17
• [Presentation] A unifying model for the role of polyamines in bacterial cell growth, the polyamine modulon (2007)
  • Author(s): Igarashi, K.
  • Organizer: Gordon Research Conference on Polyamines
  • Place of Presentation: Waterville Valley, NH, USA
  • Year and Date: 2007-07-17
• [Remarks] ホームページ等
  • URL: http://www.p.chiba-u.ac.jp/lab/rinka/